This was done as previously reported by us (13 (link)). In summary, the myeloma cells were treated with drugs for 24 hours and cytospun slides were made. The cells were fixed in 4% paraformaldehyde followed by blocking using 5% goat serum. It was further incubated with primary antibodies such as IKZF1 (Santa Cruz Biotechnology) overnight at 4°C. The slides were rinsed with PBS thrice and incubated with secondary antibodies (anti-mouse) conjugated with Alexa Fluor 594, (Invitrogen) for 1 hour. The slides were again washed, air dried, and counterstained with DAPI containing mountant (Vectashield). The images were acquired in fluorescence microscope (Axioimager M1, Carl Zeiss) at 100 × with oil immersion and images were analyzed using ISIS Metasystem (Metasystems GmbH).
Isis metasystem
Manufactured by MetaSystems
Sourced in Germany
The ISIS Metasystem is a comprehensive laboratory equipment solution designed for scientific research and analysis. The core function of the ISIS Metasystem is to provide a integrated platform for data acquisition, processing, and management across various analytical instruments and techniques.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using isis metasystem
1
Immunofluorescence Analysis of IKZF1 in Myeloma Cells
2
Leukemic Cell-Stromal Interaction Analysis
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The leukemic cells were co-cultured with stromal cells, the cells were carefully pipetted out and cytospin slides were made after 6 h of co-culture. The cells were fixed in 4% paraformaldehyde followed by blocking using 5% goat serum. It was further incubated with primary antibodies such as p65 (Santa Cruz, Dallas, USA) overnight at 4 °C. The slides were rinsed with PBS thrice and incubated with secondary antibodies (anti-mouse) conjugated with alexaflour 594, (Invitrogen, California, USA) for 1 h. The slides were again washed, air dried and counterstained with DAPI containing mountant (Vectashield, California, USA). The images were acquired in fluorescence microscope (Axioimager M1, Carl Zeiss, Germany) at ×100 with oil immersion and images were analyzed using ISIS metasystem, (Metasystems GmbH, Altlussheim, Germany).
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