Mdivi 1

Adult, male Sprague Dawley rats (300–400 g) were purchased from Charles River Laboratories (Wilmington, MA, USA). Mdivi-1 was purchased from Tocris Bioscience (Bristol, UK). Antibodies for Drp1 (westerns) and Doublecortin (DCX) were purchased from Cell Signaling Technology (Danvers, MA, USA) whereas Drp1 (immuno-gold), TOMM20 and GAPDH antibodies were purchased from Abcam (Cambridge, MA, USA).

Mdivi-1 was obtained from Tocris bioscience (American). Lipopolysaccharide (LPS) were purchased from Sigma (St. Louis, MO, United States). H9C2 cell line was purchased from Biowit Technologies (Shenzhen, China). DMEM were from Gibco/Invitrogen. ATP content and JC-1 test kit were from Beyotime. Mito-Tracker™ Deep Red FM was obtained from Invitrogen. The kits of measurement of TNFα, IL-6, D-lactate, ROS, MDA, and SOD were from Nanjing Jiancheng Co., Ltd. ROS Detection Kit of cells were purchased from Abcam (Cambridge, MA, United States). Albumin-fluorescein isothiocyanate conjugate (FITC-BSA) and collagenase type Ⅱ were purchased from Sigma (St. Louis, MO, United States). NF-κ B p65 antibody (Abcam, 1:1000), NF-κ B p65 phosphorylation antibody (Abcam, 1:1000), GAPDH antibody (Thermo, 1:1000), β-actin antibody (Thermo, 1:7000). Tunel was purchased from Roche.