Python tissue dissociation system

Manufactured by Singleron Biotechnologies

Sourced in China

The PythoN™ Tissue Dissociation System is a laboratory equipment designed for the efficient dissociation of tissue samples. It utilizes a proprietary dissociation mechanism to effectively break down tissue into a single-cell suspension.

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Lab products found in correlation

Scellive tissue preservation solution, by Singleron Biotechnologies (5 mentions) Scellive tissue dissociation solution, by Singleron Biotechnologies (5 mentions) Gexscope red blood cell lysis buffer rclb, by Singleron Biotechnologies (3 mentions) Matrix single cell processing system, by Singleron Biotechnologies (1 mentions) Novaseq 6000, by Illumina (1 mentions) Gexscope red blood cell lysis buffer, by Singleron Biotechnologies (1 mentions)

Close spelling variants of this product

PythoN™ Automated Tissue Dissociation System Singleron PythoN™ Automated Tissue Dissociation System

5 protocols using python tissue dissociation system

Single-cell RNA Sequencing of Spleen Tissue

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The fresh spleen tissues were stored in the sCelLive™ Tissue Preservation Solution (Singleron, China) on ice after the surgery within 30 min, and then digested with 3 mL sCelLive™ Tissue Dissociation Solution (Singleron) by PythoN™ Tissue Dissociation System (Singleron) at 37 °C for 15 min. The cell suspension was collected and filtered through a 40-micron sterile strainer. Afterwards, the red blood cell lysis buffer (RCLB, Singleron) was added and incubated at room temperature for 5-8 min. The mixture was then centrifuged to remove supernatant and suspended softly with PBS.
Single-cell suspensions (2 × 10⁵ cells/mL) with PBS were loaded onto a microwell chip using the Matrix® Single Cell Processing System (Singleron). Barcoding beads are subsequently collected from the microwell chip, followed by reverse transcription of the mRNA captured by the beads to obtain cDNA for PCR amplification. The amplified cDNA is then fragmented and ligated with sequencing adapters. The scRNA-seq libraries were constructed according to the protocol of the Single Cell RNA Library Kits (Singleron). Individual libraries were diluted to 4 nM, pooled, and sequenced on an Illumina Novaseq 6000 (Illumina, USA) instrument with a 150 bp paired-end format. Full identified sequencing data are available in the gene expression omnibus (GEO) under accession number GSE234756.

Xiong F., Zhang C., Shang B., Zheng M., Wang Q., Ding Y., Luo J, & Li X. (2023). An mRNA-based broad-spectrum vaccine candidate confers cross-protection against heterosubtypic influenza A viruses. Emerging Microbes & Infections, 12(2), 2256422.

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Single-Cell Isolation from Fibrotic and Healthy Livers

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The livers obtained from fibrotic liver of S. japonicum-induced mouse and healthy liver of normal mouse. The fresh tissues were stored in the sCelLive™ Tissue Preservation Solution (Singleron) on ice as quickly as possible. After washed with Hanks Balanced Salt Solution (HBSS) for three times, the specimens were minced into small pieces. To dissociated into single-cell suspensions, the specimens then digested with 3 mL sCelLive™ Tissue Dissociation Solution (Singleron) by Singleron PythoN™ Tissue Dissociation System at 37°C for 15 min. After filtered through a 40-micron sterile strainer, the cell was added the GEXSCOPE^® red blood cell lysis buffer (RCLB, Singleron), which was incubated at room temperature for 5-8 min to remove red blood cells. Centrifugation was performed at 300 × g 4°C for 5 mins to remove supernatant and single cells were resuspended softly in PBS. Finally, the cell viability was evaluated microscopically through staining with Trypan Blue.

Zhang Y., Li J., Li H., Jiang J., Guo C., Zhou C., Zhou Z, & Ming Y. (2022). Single-cell RNA sequencing to dissect the immunological network of liver fibrosis in Schistosoma japonicum-infected mice. Frontiers in Immunology, 13, 980872.

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Isolation of Renal Cell Populations

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Within 30 min of procurement from both LN and normal donors, the renal tissue samples were preserved in the sCelLiveTM Tissue Preservation Solution (Singleron, China) on ice. The samples were then rinsed three times with Hanks Balanced Salt Solution, cut into small pieces, and digested using 3 mL of sCelLiveTM Tissue Dissociation Solution with the Singleron PythoN Tissue Dissociation System at 37°C for 15 min. The cell mixture was gathered and passed through a 40‐micron sterile filter to separate the cells. After that, the GEXSCOPE red blood cell lysis buffer (RCLB, Singleron) was added to the mixture in a 1:2 (volume) ratio with the cells and incubated at room temperature for 5–8 min to eliminate red blood cells. The mixture was then centrifuged at 300xg and 4°C for 5 min to remove the supernatant and resuspended softly in phosphate buffered saline (PBS) (HyClone, USA).

Tang Y., Zhang Y., Li X., Xu R., Ji Y., Liu J., Liu J., Zhuang Q, & Zhang H. (2023). Immune landscape and the key role of APOE+ monocytes of lupus nephritis under the single‐cell and spatial transcriptional vista. Clinical and Translational Medicine, 13(4), e1237.

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Single-cell Isolation from Fresh Tissues

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The fresh tissues were preserved in the sCelLiVE Tissue Preservation Solution (Singleron) on ice within 30 min post-surgery. Following this, the specimens underwent a triple wash with Hanks Balanced Salt Solution, were minced into small pieces, and subsequently digested with 3 mL of sCelLiVE Tissue Dissociation Solution (Singleron) using the Singleron PythoN Tissue Dissociation System at 37°C for 15 min. The resulting cell suspension was collected and passed through a 40 micron sterile strainer. Next, GEXSCOPE red blood cell lysis buffer (RCLB, Singleron) was introduced to the cell suspension. This mixture, in a ratio of one part cell to two parts RCLB by volume, was incubated at room temperature for 5–8 min to eliminate red blood cells. After incubation, the mixture underwent centrifugation at 300×g at 4°C for 5 min to remove the supernatant. The remaining pellet was gently resuspended in PBS.

Long X., Zhang Z., Li Y., Deng K., Gao W., Huang M., Wang X., Lin X., She X., Zhao Y., Zhang M., Huang C., Wang S., Du Y., Du P., Chen S., Liu Q, & Wu M. (2024). ScRNA-seq reveals novel immune-suppressive T cells and investigates CMV-TCR-T cells cytotoxicity against GBM. Journal for Immunotherapy of Cancer, 12(4), e008967.

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Tissue Dissociation and Single-Cell Isolation

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Fresh tissues were stored on ice in the sCelLive™ Tissue Preservation Solution (Singleron) within 30 min after surgery. Hanks Balanced Salt Solution (HBSS) was used three times to wash the specimens, mince them into small pieces, and digest them in 3 mL sCelLive™ Tissue Dissociation Solution (Singleron) by Singleron PythoN™ Tissue Dissociation System at 37°C for 15 min. A 40-micron sterile strainer was used to collect and filter the cell suspension. After adding the GEXSCOPE^® red blood cell lysis buffer (RCLB, Singleron), the mixture [Cell: RCLB = 1:2 (volume ratio)] was incubated for 5–8 min at room temperature to remove red blood cells. After centrifuging at 300 × g 4°C for 5 min, the mixture was suspended in PBS softly after centrifugation. Finally, Trypan Blue staining was applied to the samples, and cell viability was assessed microscopically.

Huang X., Lu Z., Jiang X., Zhang Z., Yan K, & Yu G. (2023). Single-cell RNA sequencing reveals distinct tumor microenvironment of ground glass nodules and solid nodules in lung adenocarcinoma. Frontiers in Cell and Developmental Biology, 11, 1198338.

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