The FITC Annexin-V/Dead Cell Apoptosis Kit with FITC annexin V and propidium iodide (PI) (Invitrogen, Molecular Probes , USA) for flow cytometry provides a rapid and convenient assay for apoptosis. In this protocol, 1×105 cells were seeded and washed twice with PBS, and then mixed with 100 μL binding buffer to form a cell suspension. FITC annexin-V 5μL was mixed with 1 μL of 100 μg/mL PI working solution. The cells were incubated in the dark for 15 minutes at room temperature. 400 μL binding buffer was then added, mixed gently and analyzed immediately using an FACS.
Fitc annexin 5 dead cell apoptosis kit with fitc annexin 5 and propidium iodide
Manufactured by Thermo Fisher Scientific
Sourced in United States
The FITC Annexin-V/Dead Cell Apoptosis Kit with FITC annexin V and propidium iodide (PI) is a laboratory equipment used to detect and quantify apoptosis and necrosis in cell populations. The kit contains FITC-conjugated Annexin V, which binds to phosphatidylserine exposed on the surface of apoptotic cells, and propidium iodide, which stains the DNA of dead cells.
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2 protocols using fitc annexin 5 dead cell apoptosis kit with fitc annexin 5 and propidium iodide
1
Apoptosis Quantification by Flow
2
Apoptosis Detection in Renal Cancer Cells
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The FITC Annexin-V/Dead Cell Apoptosis Kit with FITC annexin-V and propidium iodide (PI) (Invitrogen, Molecular Probes, Carlsbad, CA, USA) for flow cytometry provides a rapid and convenient assay for apoptosis. Briefly, 1 × 105 stable renal cancer cells were collected and washed twice with PBS and then mixed with 100 μL binding buffer to form a cell suspension. FITC annexin-V 5 μL was mixed with 1 μL of 100 μg/mL PI working solution. The cells were incubated in the dark for 15 min at room temperature. Then, 400 μL binding buffer was added, mixed gently, and analyzed immediately using an FACS.
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