Cryopreserved cells were thawed, washed and permeabilized with a Transcription Factor perm/wash buffer (eBioscience). Cells were then stained at room temperature for 45 minutes with the following antibodies: CD3 BV650 (OKT3; Biolegend), CD4 BV785 (OKT4; Biolegend), CD8 BV510 (RPA-T8; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), HLA-DR BV605 (L243; Biolegend), Killer cell Lectin-like Receptor G1 (KLRG1) PerCP-eFluor 710 (13F12F2, eBioscience), Eomes eFluor 660 (WD1928, eBioscience), IFNγ BV711 (4S.B3; Biolegend), TNFα eFluor 450 (Mab11; Biolegend), IL-2 PE/Dazzle (MQ1-17H12, Biolegend) and CD153 (R&D116614, R&D). Samples were acquired on a BD LSR-II and analyzed using FlowJo (v9.9.6, TreeStar). A positive cytokine response was defined as at least twice the background of unstimulated cells. To define the phenotype of Mtb300-specific cells, a cut-off of 30 events was used. The gating strategy is presented in Supplementary Fig. 5 .
Hla dr bv605 l243
Manufactured by BioLegend
Sourced in United States
HLA-DR BV605 (L243) is a fluorophore-conjugated anti-human monoclonal antibody that binds to the HLA-DR antigen. It can be used for the identification and enumeration of HLA-DR-expressing cells in flow cytometric analysis.
Automatically generated - may contain errors
Lab products found in correlation
Transcription factor perm wash buffer, by Thermo Fisher Scientific (4 mentions)
Cd27 pe cy5 1a4cd27, by Beckman Coulter (4 mentions)
Ifnγ bv711 4s b3, by BioLegend (4 mentions)
Cd4 bv785 okt4, by BioLegend (4 mentions)
Cd3 bv650 okt3, by BioLegend (4 mentions)
Cd8 bv510 rpa t8, by BioLegend (3 mentions)
Il 2 pe dazzle mq1 17h12, by BioLegend (3 mentions)
Lsr 2, by BD (3 mentions)
Klrg1 percp efluor 710 13f12f2, by Thermo Fisher Scientific (2 mentions)
Eomes efluor 660 wd1928, by Thermo Fisher Scientific (2 mentions)
Tnfα efluor 450 mab11, by BioLegend (2 mentions)
Cd153, by R&D Systems (2 mentions)
Ki67 percpcy5.5 b56, by BD (2 mentions)
Tnf α pe cy7 mab11, by BioLegend (2 mentions)
Mip 1β alexa fluor 488, by R&D Systems (1 mentions)
Cd38 apc hit2, by BD (1 mentions)
Cd19 bv750 hib19, by BioLegend (1 mentions)
Cd8 bv510 rpa 8, by BioLegend (1 mentions)
4 protocols using hla dr bv605 l243
1
Phenotypic Analysis of Mycobacterial-Specific T Cells
2
Multiparametric Phenotyping of Mtb300-Specific T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cryopreserved cells were thawed, washed and permeabilized with a Transcription Factor perm/wash buffer (eBioscience). Cells were then stained at room temperature for 45 minutes with the following antibodies: CD3 BV650 (OKT3; Biolegend, San Diego, CA, USA), CD4 BV785 (OKT4; Biolegend), CD8 BV510 (RPA-T8; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter, Brea, CA, USA), HLA-DR BV605 (L243; Biolegend), Ki67 PerCPcy5.5. (B56, BD), Granzyme B (GrB) BV421 (GB11, BD), Killer cell Lectin-like Receptor G1 (KLRG1) APC (13F12F2, eBioscience), IFN-γ BV711 (4S.B3; Biolegend), TNF-α PEcy7 (Mab11; Biolegend), IL-2 PE/Dazzle (MQ1-17H12, Biolegend), Mip-1β Alexa Fluor 488 (#24006, R&D systems, Minneapolis, MN, USA) and CD153 (R&D116614, R&D). Samples were acquired on a BD LSR-II and analyzed using FlowJo (v9.9.6, FlowJo LCC, Ashland, OR, USA). The granulocytes/monocytes population was defined based on their FSC/SSC characteristics. A positive cytokine response was defined as at least twice the background of unstimulated cells. To define the phenotype of Mtb300-specific cells, a cut-off of 30 events was used.
3
Multiparametric Flow Cytometry Profiling
4
Phenotypic Analysis of Mtb300-specific T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cryopreserved cells were thawed, washed and permeabilized with a Transcription Factor perm/wash buffer (eBioscience). Cells were then stained at room temperature for 45 min with the following antibodies: CD3 BV650 (OKT3; Biolegend), CD4 BV785 (OKT4; Biolegend), CD8 BV510 (RPA-T8; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), HLA-DR BV605 (L243; Biolegend), Killer cell Lectin-like Receptor G1 (KLRG1) PerCP-eFluor 710 (13F12F2, eBioscience), Eomes eFluor 660 (WD1928, eBioscience), IFNγ BV711 (4S.B3; Biolegend), TNFα eFluor 450 (Mab11; Biolegend), IL-2 PE/Dazzle (MQ1-17H12, Biolegend), and CD153 (R&D116614, R&D). Samples were acquired on a BD LSR-II and analyzed using FlowJo (v9.9.6, TreeStar). A positive cytokine response was defined as at least twice the background of unstimulated cells. To define the phenotype of Mtb300-specific cells, a cut-off of 30 events was used. The gating strategy is presented in Supplementary Fig. 5 .
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