Binding affinity was determined using FP. Peptide 8 (10 nM) was added to serial dilutions of GST-β-catenin in 20 mM Tris, 300 mM NaCl, pH 8.8, 0.01% Triton-X100 as reported previously.34 (link) After 1 h, aliquots were transferred from the reaction mixtures into black-on-black 384-well non-binding plates (Greiner) and FP was measured using a Tecan Infinite M1000 Pro plate reader. The KD value was calculated using KaleidaGraph v. 3.6 using the above equation.
Infinite m1000 pro plate
Manufactured by Tecan
The Infinite M1000 Pro is a multi-mode microplate reader designed for high-performance absorbance, fluorescence, and luminescence measurements. It features a flexible and modular design to accommodate a wide range of microplate formats and sample types.
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Lab products found in correlation
2 protocols using infinite m1000 pro plate
1
Fluorescence Polarization Assay for Binding Affinity
2
ATP Levels Quantification in Cells
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ATP levels were measured using a Cellno ATP Assay Kit (Toyo B‐Net Co., Ltd) according to the manufacturer’s protocol. Prior to measurement, cells were cultured in IMDM medium with or without reagents for 45 minutes. Luminescence intensity was measured using an Infinite M1000 Pro plate reader (Tecan).
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