BalB-C female nude mice (6 weeks old) were purchased from Janvier Labs (Saint Berthevin, France). BT-474 cells (10 × 106 in 0.15 mL sterile PBS) were injected subcutaneously on the right flank of BalB-C nude mice under gas anesthesia. The procedures were approved by the local ethic committee (CEEA Val de Loire) and by the French Ministry of National Education, Higher Education and Research (No. 201501091617160v6). Tumor-bearing mice were separated into three distinct groups when tumor volumes were closed to 150 mm3.
Female balb c nude mice
Manufactured by Janvier Labs
Sourced in France
Female BALB/c nude mice are athymic mice that lack a functional thymus gland, resulting in a compromised immune system. They can be used for a variety of research purposes that require an immunodeficient animal model.
Automatically generated - may contain errors
Lab products found in correlation
10 protocols using female balb c nude mice
1
Subcutaneous Tumor Xenograft in Nude Mice
2
Bone Metastasis Model in Nude Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Four-week-old BALB/c female nude mice (Janvier Labs®, Le Genest St Ile France) (n = 5) were purchased and housed for 2 weeks in a specific-pathogen-free facility (ALECS platform, Faculté de Médecine Laennec, Lyon, France). After two weeks of housing, mice were anesthetized with intraperitoneal injection of 20 µL/g of ketamine 130 mg/kg and xylazine 8.8 mg/kg diluted in physiological serum and injected intracardiac with 100 µL of A549 tumor cell solution (1*106 cells/mL of sterile PBS). The development of bone metastases was assessed weekly on plain radiographs (X-rays set-up: 18 kV-35 s; Faxitron®, Tucson, AZ USA). At day 30, mice were sacrificed and hind limbs were then collected, fixed with paraformaldehyde 4% for 24 h, rinsed with PBS and then processed according to the routine histology procedure. Four-micron paraffin sections were obtained and stained with hematoxylin and eosin. Mice protocol followed was approved by the animal ethics committee of Lyon I university (IRB approval number: BH2012-05).
3
Monitoring Lung Metastasis Inhibition by Omomyc
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
a suspension of 500,000 luciferase-expressing MDA-MB-231-Omomyc cells in PBS were inoculated through the lateral tail vein of 20 BALB/c nude female mice (Janvier). Starting the day after inoculation, lung colonization and growth of tumor cells was monitored weekly by IVIS imaging. One week after inoculation, mice were randomized into two groups and treated with either 2 g/L doxycycline and 5% sucrose in the drinking water (n = 10) or with 5% sucrose only (n = 10). Five weeks later, a final in vivo bioluminescence detection was performed. Moreover, micro-CT (μCT) scans were performed to allow visualization of individual lung lesions. Mice were euthanized, and their lungs excised, fixed for 48 hours in buffered 4% formaldehyde, transferred to 70% ethanol and embedded in paraffin. Sections were cut 3.5-μm thick and stained with hematoxylin and eosin (H&E). Metastatic foci were counted and their area measured with ImageJ.
4
Omomyc Biodistribution in Tumor Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Omomyc was labeled with BDP-650/665 maleimide (Lumiprobe #58480) with an efficiency of 70%.
Twenty BALB/c nude female mice (Janvier) were used for the study. 10 mice received 0.5 million cells intravenously and 10 mice received 1.5 million cells orthotopically as described before to induce the formation of lung and mammary tumors, respectively. Lung tumor growth was followed by weekly IVIS imaging and mammary tumor growth was followed by caliper measurements. Seven weeks after inoculation, mice were treated with 50 mg/kg of Omomyc-BDP-650/665 and euthanized after 1 hour. Two mice from each model were treated with vehicle only and served as controls. Lungs and mammary fat pads were analyzed ex vivo by IVIS imaging and the signal emitted by labeled Omomyc was quantified.
Twenty BALB/c nude female mice (Janvier) were used for the study. 10 mice received 0.5 million cells intravenously and 10 mice received 1.5 million cells orthotopically as described before to induce the formation of lung and mammary tumors, respectively. Lung tumor growth was followed by weekly IVIS imaging and mammary tumor growth was followed by caliper measurements. Seven weeks after inoculation, mice were treated with 50 mg/kg of Omomyc-BDP-650/665 and euthanized after 1 hour. Two mice from each model were treated with vehicle only and served as controls. Lungs and mammary fat pads were analyzed ex vivo by IVIS imaging and the signal emitted by labeled Omomyc was quantified.
5
Omomyc Impacts Lung Metastasis in TNBC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MDA-MB-231 cells were treated with 20 μmol/L Omomyc or an equivalent volume of vehicle and, after 3 days, trypsinized for 30 minutes with 0.25% trypsin to remove any potential membrane-bound Omomyc. 500,000 cells in PBS previously treated with vehicle were inoculated through the lateral tail vein of 15 BALB/c nude female mice (Janvier), and 500,000 cells in PBS previously treated with Omomyc were inoculated into 15 other female mice. After 24 days, mice were euthanized and their lungs excised, fixed for 48 hours in buffered 4% formaldehyde, transferred to 70% ethanol, and embedded in paraffin. Sections were cut 3.5-μm thick and stained with H&E. Whole lung sections were scanned with a digital slide scanner (Hamamatsu Photonics) and metastatic foci were counted and their area measured with the NDP.view2 viewing software.
6
Subcutaneous Xenograft Model in BALB/c Nude Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female BALB/c nude mice (Janvier) (age, 6–8 wk) were housed in individually ventilated cages (6 mice per cage) under nonsterile conditions with ad libitum access to chlorophyll-free animal chow and water. CHL-GLP-1R cells were injected subcutaneously on the right shoulder (5 × 106 cells/mouse) in 200 μL of Dulbecco modified Eagle medium with 4.5 g/L d -glucose and GlutaMAX. All animal experiments were approved by the institutional Animal Welfare Committee of the Radboud University Medical Centre and were conducted in accordance with the guidelines of the Revised Dutch Act on Animal Experimentation.
7
Balb/C Nude Mice Anesthesia Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female Balb/C nude mice (three animals per group for two groups) were purchased from Janvier Laboratories with a weight of around 20 g/individual (7 weeks). During the acquisition of in vivo imaging, the animals were anesthetized by a mixture of isoflurane/air (Iso-Vet, Piramal Healthcare, Mumbai, India) at 5% (v/v) and maintained at 2.5% (v/v). All experiments on animals were performed in accordance with institutional guidelines for animal use specified by the French Ministry of Higher Education, Research and Innovation (APAFIS#29222-2020121807569293 v6).
8
Xenograft Model of CHL-GLP-1R Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female BALB/c nude mice (Janvier), 6–8 wk old, were housed in individually ventilated cages (6 mice per cage) under nonsterile conditions with ad libitum access to chlorophyll-free animal chow and water. CHL-GLP-1R cells (5 × 106 cells per mouse in 200 μL of Dulbecco modified Eagle medium with 4.5 g/L d -glucose and GlutaMAX) were injected subcutaneously on the right flank of the mice.
9
Xenograft Mouse Model of Insulinoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal experiments were conducted in accordance with the German animal protection law (TierSchG). The protocol was approved by the Animal Welfare Ethics committees of the University of Freiburg (Regierungspraesidium Freiburg Az G-12/21).
Female BALB/c nude mice (weight, 18-20 g; age, 6-8 wk) were obtained from Janvier Labs and were housed and handled in accordance with the good animal practice as defined by FELASA and the national animal welfare body GVSOLAS. Xenografts were established on the right shoulder by subcutaneous injection of 5 million rat Ins-1E cells (26) in 1:1 v/v mixture of phosphate-buffered saline and Matrigel (final volume, 100 mL) under isoflurane anesthesia. Mice were fed 60% Glucose Diet (PROVIMI KLIBA SA).
Female BALB/c nude mice (weight, 18-20 g; age, 6-8 wk) were obtained from Janvier Labs and were housed and handled in accordance with the good animal practice as defined by FELASA and the national animal welfare body GVSOLAS. Xenografts were established on the right shoulder by subcutaneous injection of 5 million rat Ins-1E cells (26) in 1:1 v/v mixture of phosphate-buffered saline and Matrigel (final volume, 100 mL) under isoflurane anesthesia. Mice were fed 60% Glucose Diet (PROVIMI KLIBA SA).
10
Subcutaneous Tumor Induction in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal experiments were conducted according to the regulations of the University Medical Center of Freiburg. Female BALB/c nude mice (18-20 g, 6-8 wk old) were obtained from Janvier Labs. In separate animals, Karpas 299 CD30-positive and A-431 CD30-negative tumors were induced on the right shoulder by subcutaneous injection of 20-25 million Karpas 299 cells in a 1:1 v/v mixture of PBS and Matrigel (Corning) or 5 million A-431 cells in PBS (final volume, 100 mL).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!