Hnrnpu

Manufactured by Thermo Fisher Scientific

Sourced in United States

HNRNPU is a protein that functions as a regulator of mRNA processing and transport. It is involved in various cellular processes, including gene expression, RNA splicing, and nuclear-cytoplasmic transport. HNRNPU plays a role in the regulation of gene expression and is important for cellular function.

Automatically generated - may contain errors

Lab products found in correlation

Fam171b, by Novus Biologicals (2 mentions) Vimentin, by Abmart (2 mentions) Taqman probe, by Thermo Fisher Scientific (2 mentions) Ubiquitin, by Proteintech (1 mentions) E cadherin, by Proteintech (1 mentions) N cadherin, by Proteintech (1 mentions) Vimentin, by Proteintech (1 mentions) β tubulin, by Abmart (1 mentions) Gapdh, by Abmart (1 mentions) β actin, by Abmart (1 mentions) Cd206, by Proteintech (1 mentions) F4 80, by Proteintech (1 mentions) α sma, by Proteintech (1 mentions) C myc, by Thermo Fisher Scientific (1 mentions) 7500 ht real time pcr instrument, by Thermo Fisher Scientific (1 mentions) Gapdh, by Thermo Fisher Scientific (1 mentions) Taqman gene expression master mix, by Thermo Fisher Scientific (1 mentions) Omniscript rt kit, by Qiagen (1 mentions) Dulbecco modified eagle medium (dmem), by Avantor (1 mentions) Uea 1, by Vector Laboratories (1 mentions)

4 protocols using hnrnpu

Western Blot Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The western blot assay was performed as previously described. Antibody information: FAM171B (Novusbio, NBP1-93847); vimentin (Abmart, T55134); HNRNPU (Thermofisher, PA5-63604); ubiquitin (Proteintech, 10201-2); vimentin (Proteintech, 10366-1); E-cadherin (Proteintech, 20874-1); N-cadherin (Proteintech, 22018-1); HA (Abmart, M20003); DYKDDDDK (Abmart, M20008); GAPDH (Abmart, M20006); β-tubulin (Abmart, M20005); β-actin (Abmart, P30002).

Hu W.M., Li M., Ning J.Z., Tang Y.Q., Song T.B., Li L.Z., Zou F., Cheng F, & Yu W.M. (2023). FAM171B stabilizes vimentin and enhances CCL2-mediated TAM infiltration to promote bladder cancer progression. Journal of Experimental & Clinical Cancer Research : CR, 42, 290.

+ Open protocol

+ Expand

Immunoassay protocol for protein analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The immunofluorescent (IF) assay, immunohistochemical (IHC) assay, and hematoxylin-eosin (HE) assay were performed as previously described. Antibody information: FAM171B (Novusbio, NBP1-93847); vimentin (Abmart, T55134); HNRNPU (Thermofisher, PA5-63604); CD206 (Proteintech, 18704-1); CD8 (Proteintech, 66868-1); α-SMA (Proteintech, 14395-1); CD68 (Proteintech, 28058-1); F4/80 (Proteintech, 29414-1); ARG1 (Proteintech, 16001-1). For immunofluorescent colocalization analysis, the Colocalization Finder and Scatter J plugins of Image J were employed. The criteria for colocalization were set as follows: Pearson correlation coefficient (r) > 0.5 and Manders’ colocalization coefficients (M1 and M2) > 0.5.

+ Open protocol

+ Expand

Quantitative Real-Time PCR for mRNA and pri-miRNA Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

For mRNA and pri-miR expression analysis, 0.5 μg RNA was reversely transcribed to cDNA by use of the Omniscript RT Kit (Qiagen, Cat. # 205111) with Primer “random” (Roche, Cat. # 11034731001), following the manufacturer’s protocol. The cDNA was amplified using 1 µL of the cDNA preparation, the Taqman Gene Expression Master Mix (Applied Biosystems, Cat. # 4440040), and Taqman probes for hnRNPU, c-myc, GAPDH, and 18S (all from Thermo Fisher Scientific: hnRNPU Cat. # 4331182, Assay ID Hs00244919_m1; GAPDH, Cat. # 4331182, Assay ID Hs02786624_g1; c-myc, Cat. # 4453320, Assay ID Hs00153408_m1; 18S, Cat. # 4331182, Assay ID Hs99999901_s1; pri-miR-30c-1, Cat. # 4427012, Assay ID Hs03303371_pri; pri-miR-30c-2 Cat. # 4427012, Assay ID Hs03302833_pri) in a 7500 HT real-time PCR instrument (Applied Biosystems). GAPDH was used as an internal control and relative expression was calculated as ΔΔCT values.

Zietzer A., Hosen M.R., Wang H., Goody P.R., Sylvester M., Latz E., Nickenig G., Werner N, & Jansen F. (2020). The RNA-binding protein hnRNPU regulates the sorting of microRNA-30c-5p into large extracellular vesicles. Journal of Extracellular Vesicles, 9(1), 1786967.

+ Open protocol

+ Expand

Cell Line Authentication and Mycoplasma Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

All cell lines used in this manuscript were validated for identity by the H. Lee Moffitt Cancer Center Molecular Genomics Core within 2 years of testing and were confirmed to be mycoplasma-free using the Plasmo Test kit (InvivoGen, San Diego, CA, USA). Cell lines were cultured in DMEM (VWR, Radnor, PA, USA) supplemented with heat inactivated fetal bovine serum 1:10 (Peak Serum, Denver, CO, USA). Antibodies: RPS3 (Bethyl, Montgomery, TX, USA), HNRNPU (ThermoFisher, Waltham, MA, USA), BCLAF1 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), EIF3K (Bethyl), FLAG (Sigma, St. Louis, MO, USA), β-actin (Sigma), β-tubulin (Developmental Studies Hybridoma Bank, DSHB, Iowa City, IA, USA), LaminA/C (DSHB). UEAI, AAL, UEAI-488, AAL-488, UEAI-biotin, AAL-biotin were purchased from Vector Laboratories (Burlingame, CA, USA). Secondary antibodies: anti-rabbit-HRP (Santa Cruz Biotechnology, Inc.), m-IgGκ-BP-HRP (Santa Cruz Biotechnology, Inc.), streptavidin-800 (Life Technologies, Carlsbad, CA, USA), IRDye 680RD anti-mouse (LI-COR, Lincoln, NE, USA).

Watson G., Lester D., Ren H., Forsyth C.M., Medina E., Gonzalez Perez D., Darville L., Yao J., Luca V., Koomen J., Cen L, & Lau E. (2021). Fucosylated Proteome Profiling Identifies a Fucosylated, Non-Ribosomal, Stress-Responsive Species of Ribosomal Protein S3. Cells, 10(6), 1310.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!