The sections were treated with rat anti‐F4/80 (Ab6640; 1:50; Abcam, Shanghai, China), mouse anti‐CD86 (NBP2‐44515, 1:50; Novus, Shanghai, China), and rabbit anti‐CD206 (Ab195191, 1:50; Abcam) primary antibodies (15 hours, 4℃) followed by staining with Cy3‐labeled goat anti‐rat IgG (Bs‐0293G‐CY3; 1:100, Bioss, Beijing, China), FITC‐labeled goat anti‐mouse IgG (BA1101, 1:100; Boster Biological Technology Co, Ltd, Wuhan, China), and FITC‐labeled goat anti‐rabbit IgG (BA1105, 1:10; Boster Biological Technology Co, Ltd) secondary antibodies (1 hour, 20℃), respectively. The sections were then treated with 4,6‐diamino‐2‐phenylindole (DAPI, C1002; Beyotime Biotechnology Co Ltd, Wuhan, China) to stain the nuclei (5 minutes, 20℃). The proportion of M1 and M2 macrophages was evaluated using the mean optical density (MOD) as determined by the ImageJ analysis software.
Bs 0293g cy3
Manufactured by Bioss Antibodies
Sourced in China
The Bs-0293G-CY3 is a fluorescent-labeled antibody product from Bioss Antibodies. It is designed for use in various research applications that require the detection and visualization of target proteins or molecules.
Automatically generated - may contain errors
2 protocols using bs 0293g cy3
1
Quantifying M1 and M2 Macrophages
2
Immunofluorescence Analysis of Kidney Tissue
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The kidney tissue was embedded and cut into slices with a thickness of 10 µm. After the slice was incubated in 5% BSA (SH30574.03, Hyclone) blocking solution for 1 h, the primary anti-F4/80 antibody (1:100; Abcam, ab6640, Britain) and HLA-DR antibody (1:100; Affinity, DF6475, China) were added to tissues and incubated overnight at 4°C. Next, we added secondary antibody fluorescence (FITC)-labeled goat antirabbit IgG (1:100; BA1105, Wuhan Boster Biological Technology, LTD., Wuhan, China) and fluorescence (Cy3)-labeled goat antirat IgG (1:100; bioss, bs-0293G-CY3, China) and incubated for 1 h at 37°C in the dark. Then, it was counterstained with Hoechst or DAPI for 5 min, and pictures were taken with a fluorescence microscope.
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