Hexahydrate

Manufactured by Merck Group

Sourced in United States

Hexahydrate is a chemical compound used in various laboratory applications. It functions as a source of specific elements or ions required for experimental purposes. The precise details and intended use of this product are not included in this factual description.

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Lab products found in correlation

8 protocols using hexahydrate

Electrochemical Characterization of Ruthenium Complexes

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All chemicals, including hexaammineruthenium(iii) chloride (Ru(NH₃)₆Cl₃), tris(2,2′-bipyridyl) dichlororuthenium(ii) hexahydrate (Ru(bpy)₃Cl₂), tri-n-propylamine, resorufin, ferrocenemethanol, glucose, and underivatized polystyrene spheres (1.0 μm in diameter, 10 wt%), were obtained from Sigma-Aldrich and used as received. All solutions for electrochemical measurements were prepared from deionized (DI) water (ρ ∼ 18 MΩ cm) generated by a Milli-Q Gradient water purification system (Millipore).

Ma C., Zaino III L.P, & Bohn P.W. (2015). Self-induced redox cycling coupled luminescence on nanopore recessed disk-multiscale bipolar electrodes †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5sc00433k Click here for additional data file.. Chemical Science, 6(5), 3173-3179.

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Ferric Oleate Synthesis for NC Precursor

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Ferric oleate, which is used as a precursor for the NC synthesis, was synthesized from iron(III) chloride hexahydrate (98%, Sigma-Aldrich) and sodium oleate (97%, TCI) in a solvent mixture of hexane and water^{59 (link)}. In a typical synthesis, 40 mmol of iron chloride and 120 mmol of sodium oleate was dissolved in a mixture solvent of 80 ml ethanol, 60 ml distilled water and 140 ml hexane, and was stirring at 70 °C for four hours. After cooling to room temperature, the upper organic layer was separated and washed three times with 30 ml distilled water. Then hexane was rotated evaporated in order to obtain ferric oleate as a dark red wax.

Lv Z.P., Kapuscinski M, & Bergström L. (2019). Tunable assembly of truncated nanocubes by evaporation-driven poor-solvent enrichment. Nature Communications, 10, 4228.

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Reagent Preparation for Analytical Studies

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Deionized water was used for the preparation and dilution of all solutions. The chemicals applied in this work were all of analytical grade. A Pb(II) stock solution of 1000 mg L⁻¹ was prepared from the nitrate salt and diluted daily for designed experiments. α-Benzoin oxime, iron(III) chloride, phenol, sodium fluoride, sodium phosphate, nickel(II) nitrate, hexahydrate, sodium nitrate, lead(II) nitrate, cobalt(II) nitrate, zinc(II) nitrate, sodium carbonate, potassium chloride, magnesium nitrate hexahydrate, calcium chloride, sodium sulfate, and iron(II) nitrate were purchased from Sigma (St. Louis, MO, USA). Sodium hydroxide, hydrochloric acid, nitric acid, and hydrogen peroxide were purchased from Merck (Darmstadt, Germany).

Habila M.A., AlMasoud N., Alomar T.S., AlOthman Z.A., Yilmaz E, & Soylak M. (2020). Deep Eutectic Solvent-Based Microextraction of Lead(II) Traces from Water and Aqueous Extracts before FAAS Measurements. Molecules, 25(20), 4794.

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Succinogen Actinobacillus Fermentation Protocol

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Actinobacillus succinogenes 130Z (ATCC 55618) was obtained from the American Type Culture Collection (Manassas, VA) and maintained in tryptic soy broth medium (G-Biosciences, St. Louis, MO) with 10 g/L glucose (Fisher Chemical, Hampton, NH) (TSBG hereafter). Seed cultures of A. succinogenes were started from 40% glycerol stock in 10 mL medium in 15 mL plastic conical tube and incubated at 37°C, 250 rpm for 14 hours. Fermentation studies were conducted in fermentation medium containing 16.0 g/L yeast extract (Fisher BioReagents, Pittsburgh, PA), 1.0 g/L sodium chloride (Fisher Chemical), 1.36 g/L sodium acetate (Sigma-Aldric, St. Louis, MO), 0.20 g/L magnesium chloride, hexahydrate (Sigma Life Science, St. Louis, MO), and 0.20 g calcium chloride, dihydrate (Fisher Chemical) with D-glucose (Fisher Chemical) and buffered with magnesium carbonate (Acros Organic, Morris Plains, NJ) at 80% the sugar concentration.

Long D.S., Immethun C.M., Vallecilla-Yepez L., Wilkins M.R, & Saha R. (2021). One step forward, two steps back: Transcriptional advancements and fermentation phenomena in Actinobacillus succinogenes 130Z. PLoS ONE, 16(5), e0245407.

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Lignin-Degrading Enzyme Production

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Unless otherwise stated, the chemicals and reagents used in this study were of analytical grades. Nutrient both, nutrient agar, and salts were purchased from Merck (Germany). The kraft lignin, Congo Red, ferric chloride, hexahydrate, sodium azide, pyrogallol, veratryl alcohol, guaiacol alcohol, Malachite green, Remazol Brilliant Blue, Luria broth base, pyrogallol, hydrogen peroxide, disodium salts, and glycerol were supplied by Sigma Aldrich (USA). Bacteriological agar was bought from Lasec (South Africa).

Dube S.L., Osunsanmi F.O., Ngcobo B.P., Mkhwanazi L.B., Jobe Z.Z., Aruleba R.T., Mosa R.A, & Opoku A.R. (2023). Isolation and Characterization of Potential Lignin Peroxidase-Producing Bacteria from Compost Samples at Richards Bay (South Africa). Polish Journal of Microbiology, 72(2), 117-124.

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Nanomaterial Preparation and Characterization

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Most chemicals were purchased from Sigma-Aldrich (Taufkirchen, Germany), Merck (Darmstadt, Germany), or Carl Roth (Karlsruhe, Germany) in the highest available purity.
Nanomaterials (Al 0 -core surface-passivated nanoparticles and γ-Al 2 O 3 nanoparticles) were supplied by IoLiTec (Heilbronn, Germany). Al 0 nanoparticles were stored and weighted under an argon atmosphere. Both particle species were freshly dispersed according to the modified NanoGenoTOX protocol (dispersion in 0.05% BSA/water by ultrasonication with KE76 tip for 5'09'' with ~20% energy), BSA was supplied by Carl Roth (Albumin Fraction V, ≥98%)
and AlCl 3 was supplied by Sigma-Aldrich (Hexahydrate, ≥97%).

Sieg H., Braeuning C., Kunz B.M., Daher H., Kästner C., Krause B.C., Meyer T., Jalili P., Hogeveen K., Böhmert L., Lichtenstein D., Burel A., Chevance S., Jungnickel H., Tentschert J., Laux P., Braeuning A., Gauffre F., Fessard V., Meijer J., Estrela-Lopis I., Thünemann A.F., Luch A, & Lampen A. (2018). Uptake and molecular impact of aluminum-containing nanomaterials on human intestinal caco-2 cells. Nanotoxicology, 12(9).

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Nanomaterial Preparation and Stabilization

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Standard chemicals were purchased from Sigma-Aldrich (Taufkirchen, Germany), Merck (Darmstadt, Germany), or Carl Roth (Karlsruhe, Germany) in the highest available purity.
Nanomaterials (Al 0 -core surface-passivated nanoparticles and γ-Al2O3 nanoparticles) were supplied by IoLiTec (Heilbronn, Germany). Al 0 nanoparticles were stored and weighed under an argon atmosphere. Both particles were freshly dispersed according to the modified NanoGenoTOX protocol (ultrasonication with KE76 for 5'09'' with ~20% energy), stabilized by 0.05% BSA/water before use (Krause et al., 2018) . BSA was supplied by Carl Roth (Albumin Fraction V, ≥98%) and AlCl3 was supplied by Sigma-Aldrich (Hexahydrate, ≥97%).

Sieg H., Ellermann A.L., Maria Kunz B., Jalili P., Burel A., Hogeveen K., Böhmert L., Chevance S., Braeuning A., Gauffre F., Fessard V, & Lampen A. (2019). Aluminum in liver cells - the element species matters. Nanotoxicology, 13(7).

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Synthesis and Characterization of Aluminum Nanoparticles

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Chemicals were purchased from Sigma-Aldrich (Taufkirchen, Germany), Merck (Darmstadt, Germany), or Carl Roth (Karlsruhe, Germany) in the highest available purity. Nanomaterials (Al 0 -core surface-passivated nanoparticles and γ-Al 2 O 3 nanoparticles) were supplied by IoLiTec. Al 0 nanoparticles were stored and weighted under an argon atmosphere. Both particles were freshly dispersed at a concentration of 2.56 mg/ml according to the modified NanoGenoTOX protocol (ultrasonication applying an energy of 1176 kJ/ml dispersion using an acoustic power of 7.35 W), stabilized by 0.05% BSA/water before use. BSA was supplied by Carl Roth (Albumin Fraction V, ≥98%) and AlCl 3 was supplied by Sigma Aldrich (Hexahydrate, ≥97%).

Sieg H., Kästner C., Krause B., Meyer T., Burel A., Böhmert L., Lichtenstein D., Jungnickel H., Tentschert J., Laux P., Braeuning A., Estrela-Lopis I., Gauffre F., Fessard V., Meijer J., Luch A., Thünemann A.F, & Lampen A. (2017). Impact of an Artificial Digestion Procedure on Aluminum-Containing Nanomaterials. Langmuir : the ACS journal of surfaces and colloids, 33(40).

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