D0564 1mg

Alb-TRECK/SCID mice were provided by our collaborators at the Tokyo Metropolitan Institute of Medical Science. Homozygosity was confirmed by backcrossing for at least three generations. Alb-TRECK/SCID mice were housed at Yokohama City University. Animal experimental work was conducted in accordance with the Guidelines for Proper Conduct of Animal Experiments (Science Council of Japan), and all experimental procedures were approved by the institutional review board of the Animal Research Center, Yokohama City University School of Medicine (No.075).
DT (Sigma, St Louis, MO, USA; D0564-1MG) was intraperitoneally administered (1.5 μg/kg) to 8-week-old Alb-TRECK/SCID mice, and the degree of liver damage was assessed by serum aspartate aminotransferase (AST) activity levels.

For DT-mediated depletion of KCs, Clec4f^creRosa26^LSL-DTR mice and Rosa26^LSL-DTR mice were intraperitoneally injected with 100 ng DT (D0564-1MG, Sigma-Aldrich) as a single dose or weekly injections^{25 (link)}, as indicated in the figure legends for the corresponding experiments. The efficiency of KC depletion was determined in Extended Data Fig. 1j.
For CSF1R inhibitor treatment with PLX5622³¹ , C57BL/6J mice were fed ad libitum with PLX5622-impregnated chow (1,200 mg per kg, provided by Plexxicon) or control chow 2 weeks before injection of tumour cells.
For the SIRPA blockage assay, Clec4f^creId3^f/f mice and Id3^f/f littermates were intraperitoneally injected with control IgG (HRPN, BioXcell) or 250 μg anti-SIRPA (P84, BioXcell) 2 days before and every 2 days after tumour cell injection.
For phosphatidylserine blockade^{80 (link)}, C57BL/6J mice were injected i.v. with PBS or 1 μg MFG-E8(D89E)^{81 (link)} (gift from S. Nagata), 6 h before injection of tumour cells.
For NK cell and CD8 T cell depletion, 8–12-week-old Clec4f^creId3^f/f mice and Id3^f/f littermates were intraperitoneally injected with control IgG (HRPN, BioXcell), or 200 μg anti-NK1.1 (BE0036, BioXcell) and 200 μg anti-CD8 (BP0061, BioXcell) 1 day before tumour injection and every 4 days afterwards.