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2s 3 1s 1 3 4 dichlorophenyl ethyl amino 2 hydroxypropyl phenylmethyl phosphinic acid hydrochloride cgp 55845

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(2S)-3-[[(1S)-1-(3,4-dichlorophenyl)ethyl]amino-2-hydroxypropyl] (phenylmethyl) phosphinic acid hydrochloride (CGP-55845) is a chemical compound. It functions as a selective GABA(B) receptor antagonist.

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Lab products found in correlation

Baclofen, by Merck Group (1 mentions) Pertussis toxin ptx, by Merck Group (1 mentions)

Close spelling variants of this product

CGP 55845 hydrochloride

3 protocols using 2s 3 1s 1 3 4 dichlorophenyl ethyl amino 2 hydroxypropyl phenylmethyl phosphinic acid hydrochloride cgp 55845

1

Synthesis and Properties of α-Conotoxins

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α-Conotoxins Vc1.1, cyclized-Vc1.1 (c-Vc1.1), and PeIA were synthesized as described previously (Clark et al., 2006 (link), 2010 (link); Daly et al., 2011 (link)). Synthetic Vc1.1 and PeIA are 16–amino acid residue peptides with a characteristic helical region and two disulfide bonds in a I–III, II–IV arrangement (Clark et al., 2006 (link), 2010 (link); Daly et al., 2011 (link)). c-Vc1.1 exhibits better properties than the linear Vc1.1 (also known as ACV1), including high chemical stability, resistance to cleavage by proteases, and improved potency to inhibit N-type VGCCs (Clark et al., 2010 (link)). Most data on α-conotoxin effects on various Cav2 channels were obtained using c-Vc1.1, unless otherwise noted. GABA, baclofen, guanosine 5′-[β-thio]diphosphate trilithium salt (GDP-β-S), and pertussis toxin (PTX) were purchased from Sigma-Aldrich. (2S)-3-[[(1S)-1-(3,4-dichlorophenyl)ethyl]amino-2-hydroxypropyl](phenylmethyl) phosphinic acid hydrochloride (CGP55845) and pp60c-Src peptide (521–533) were purchased from Tocris Bioscience.
Berecki G., McArthur J.R., Cuny H., Clark R.J, & Adams D.J. (2014). Differential Cav2.1 and Cav2.3 channel inhibition by baclofen and α-conotoxin Vc1.1 via GABAB receptor activation. The Journal of General Physiology, 143(4), 465-479.
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2

Microdialysis Experiments with Pharmacological Agents

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The aCSF for the microdialysis experiments was purchased from Tocris Biosciences (Bristol, UK). 3,5-dichloro-N-[1-(2,2-dimethyl-tetrahydro-pyran-4-ylmethyl)-4-fluoro-piperidin-4-yl-methyl]-benzamide (TTA-P2) was a kind gift from Merck Inc. (USA). TTA-P2 was dissolved in DMSO (2% of final solution) before addition to aCSF. An identical concentration of DMSO was added to the aCSF solutions. (2S)-3-[[(1S)-1-(3,4-dichlorophenyl)ethyl]amino-2-hydroxypropyl] (phenylmethyl) phosphinic acid hydrochloride (CGP-55845) was purchased from Tocris (UK). The pH of all dialysis solutions was 7.
McCafferty C., David F., Venzi M., Lőrincz M.L., Delicata F., Atherton Z., Recchia G., Orban G., Lambert R.C., Di Giovanni G., Leresche N, & Crunelli V. (2018). Cortical drive and thalamic feed-forward inhibition control thalamic output synchrony during absence seizures. Nature neuroscience, 21(5), 744-756.
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3

Microdialysis Experiments with Pharmacological Agents

Check if the same lab product or an alternative is used in the 5 most similar protocols
The aCSF for the microdialysis experiments was purchased from Tocris Biosciences (Bristol, UK). 3,5-dichloro-N-[1-(2,2-dimethyl-tetrahydro-pyran-4-ylmethyl)-4-fluoro-piperidin-4-yl-methyl]-benzamide (TTA-P2) was a kind gift from Merck Inc. (USA). TTA-P2 was dissolved in DMSO (2% of final solution) before addition to aCSF. An identical concentration of DMSO was added to the aCSF solutions. (2S)-3-[[(1S)-1-(3,4-dichlorophenyl)ethyl]amino-2-hydroxypropyl] (phenylmethyl) phosphinic acid hydrochloride (CGP-55845) was purchased from Tocris (UK). The pH of all dialysis solutions was 7.
McCafferty C., David F., Venzi M., Lőrincz M.L., Delicata F., Atherton Z., Recchia G., Orban G., Lambert R.C., Di Giovanni G., Leresche N, & Crunelli V. (2018). Cortical drive and thalamic feed-forward inhibition control thalamic output synchrony during absence seizures. Nature neuroscience, 21(5), 744-756.
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