Bx43 light microscope

Manufactured by Olympus

Sourced in Japan, United States, Germany

The BX43 is a light microscope designed for routine clinical and laboratory applications. It features a trinocular observation tube, allowing for the attachment of a camera or other imaging device. The microscope is equipped with high-quality optics and a sturdy, ergonomic design to provide reliable performance in a variety of settings.

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Close spelling variants of this product

BX43 microscope (497 citations) BX43 Upright Light Microscope (5 citations) BX43-DP21 light microscope (2 citations) BX43 light/fluorescence microscope (2 citations)

153 protocols using bx43 light microscope

Oxidative Stress Markers in Liver Tissue

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Immunohistochemistry was conducted using deparaffinized liver sections with antibodies against nitrotyrosine (Merk Millipore, 06-284, 1:200), phospho-histone H2A.X (Ser139) (Cell Signaling Technology, 9718, 1:100) or 8-hydroxyguanosine (8-oxoG) (Abcam, ab62623, 1:150). The percentage of nitrotyrosine⁺ area per total area was determined by using a BX43 light microscope (Olympus) and ImageJ software (NIH). The percentage of H2A.X⁺ or 8-oxoG⁺ hepatocytes among total hepatocytes was determined by manual counting under a BX43 light microscope (Olympus). All measurements were conducted in more than 25 randomly chosen fields per group.
For dihydroethidium (DHE) staining, fresh-frozen liver sections were incubated with 10 μM DHE solution (Invitrogen, D23107) for 30 min at 37°C with light protection, followed by confocal microscopy after DAPI staining. Fluorescence imaging was conducted using an LSM780 confocal microscope (Carl Zeiss). The total fluorescence of DHE⁺ cells per field was determined in more than 25 randomly chosen fields per group by using ImageJ software (NIH).

Kim J., Lee S, & Lee M.S. (2022). Suppressive Effect of Autocrine FGF21 on Autophagy-Deficient Hepatic Tumorigenesis. Frontiers in Oncology, 12, 832804.

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Pollen Grain Micromorphology Analysis

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The observations were carried out under an Olympus BX 43 light microscope equipped with a camera lucida. The SEM micrographs were taken using a Zeiss EVO 40 microscope (Carl Zeiss, Jena, Germany) at an accelerating voltage of 10-15 kV, at the Electron and Confocal Microscopy Laboratory, Faculty of Biology, Adam Mickiewicz University in Poznań. Prior to the observations, the prepared material was sputtered with gold using an SCB 050 ion sputter. The study was documented with photographs taken during observations, primarily at magnifications ranging from × 2.500 to x 3.500 for shape and x 10.000-20.000 for exine sculpture of the pollen grains. The observations were carried out in an Olympus BX 43 light microscope equipped with a camera lucida. The pollen from dehiscent anthers was fixed in a mixture of glycerol and ethanol (1:1; v/v) and then dehydrated stepwise with a graded ethanol series and finally embedded in Epon (Meek 1976 ). Next, embedded samples were cut with a Tesla 490A microtome into thin (3 µm) sections and glued to the slides with Haupt adhesive (1% gelatin in water with 2% phenol crystals and 15% glycerin). Unstained sections were examined under a Zeiss Lab. A1 fluorescence microscope (FM) in UV light (wavelength 365 nm) for exine structure analyses.

Klimko M., Nowińska R., Jura-Morawiec J., Wiland-Szymańska J, & Wilkin P. (2018). Pollen morphology of selected species of the genera Chrysodracon and Dracaena (Asparagaceae, subfamily Nolinoideae) and its systematic implications. Plant systematics and evolution = Entwicklungsgeschichte und Systematik der Pflanzen, 304(3).

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Nasal Safety Evaluation of PSO System

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In this test, the safety of the PSO nasal system containing 25% PSO at a PSO:PL ratio of 3:1 on the nasal cavity and mucosa was evaluated in rats using a method previously described by Duchi et al. [20 (link)]. In brief, nine male HSD rats (220–250 g) were divided equally into two treatment groups and one untreated control group. Rats in the treatment groups received nasally 15 μL PSO phospholipid oily system or normal saline into both nostrils twice a day, for one week. At the end of the experiment, the animals were sacrificed and the nasal cavities were removed and fixed in 3.7% formaldehyde PBS. Sections of the nasal cavity were cut serially at 5 μm thickness and stained with hematoxylin and eosin. The sections were examined by a histopathologist (Authority for Animal Facilities, Hebrew University of Jerusalem, Jerusalem, Israel) using an Olympus light microscope BX43 and an Olympus digital camera DP21 with Olympus cellSens Entry 1.13 software (Olympus, Tokyo, Japan) using a magnification of ×10. Local toxicity was assessed by evaluating the histopathological alterations in different regions of the nasal cavity including cartilage and turbinate bone, lamina propria and submucosa, mucosal epithelium, and lumen.

Natsheh H, & Touitou E. (2022). Improved Efficiency of Pomegranate Seed Oil Administrated Nasally. Pharmaceutics, 14(5), 918.

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Histological Analysis of Murine Bladder

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Age-matched WT and JNK2 KO mice were used for histological studies.
After euthanasia, WT and JNK2 KO murine bladders were fixed in 10% neutral
buffered formalin at room temperature. The fixed bladder tissue from WT and JNK2
KO mice was paraffin-embedded and five μm thick sections were stained
with Masson’s trichrome stain (MTS) using standard techniques. MTS
stained bladder sections were imaged using a Light Microscope BX43 (Olympus,
Waltham, MA, USA). Paraffin embedded bladder sections from WT and JNK2 KO mice
were subjected to deparaffinization and rehydration using xylene and ethanol.
Antigen unmasking was performed using antigen retrieval buffer (Abcam,
Cambridge, MA, USA) as per the manufacturer’s instructions. For
immunofluorescence staining, BSM strips overexpressing desmin, vimentin and GFP
were embedded in optimal cutting temperature compound (OCT: Sakura, Tokyo,
Japan) and frozen with liquid nitrogen for cryo-sectioning. A series of adjacent
sections were cut on a cryostat. Each section with 5 μM thickness was
mounted onto a charged slide.

Javed E., Thangavel C., Frara N., Singh J., Mohanty I., Hypolite J., Birbe R., Braverman A.S., Den R.B., Rattan S., Zderic S.A., Deshpande D.A., Penn R.B., Ruggieri MR S.r., Chacko S, & Boopathi E. (2019). Increased expression of desmin and vimentin reduces bladder smooth muscle contractility via JNK2. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(2), 2126-2146.

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Decalcification and Histological Analysis of Ossicles

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After dissection, the malleus, incus, and stapes bones were fixed in 10% neutral buffered formalin for 2 days, stored in 70% alcohol, and then isolated, before being placed in a labeled cassette between biopsy pads. The tissues were then immersed in DeltaFORM decalcifier (Delta Medical Inc., Brookfield, WI, USA, lot 0403.423) for two hours. The histology cassettes were removed from the decalcifier and rinsed in tap water and then loaded into a Tissue-Tek E300 processor and processed during a standard processing cycle (60 min steps). The processed cassettes were embedded and cut at 5 µm with a Leica RM 215 microtome using Tissue-Tek Feather A35 low-profile blades. Some surface decalcification was needed for some sections as the initial decalcification (a 1 h, slow process) was incomplete. The slides were deparaffinized and stained with Hematoxylin and Eosin (HE), dehydrated, and cover-slipped with mounting media. The slides were examined using an Olympus light microscope (BX 43). Images were captured with a DP 26 digital camera and the cellSens Standard image analysis software for measurements.

Martonos C.O., Gudea A.I., Ratiu I.A., Stan F.G., Bolfă P., Little W.B, & Dezdrobitu C.C. (2023). Anatomical, Histological, and Morphometrical Investigations of the Auditory Ossicles in Chlorocebus aethiops sabaeus from Saint Kitts Island. Biology, 12(4), 631.

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Histological Evaluation of Liver Disease

Cited 1 time

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Liver histology was evaluated at the end of the intervention periods; at the age of 17, 20 or 28 weeks in the steatosis model, NASH prevention and NASH treatment, respectively. Livers were perfused, isolated, fixed in 4% paraformaldehyde and embedded in paraffin. Consecutive 4 μm sections were cut and stained with hematoxylin and eosin (H&E). The presence of inflammation and steatosis score was blind evaluated by a pathologist. Scoring of liver sections was adapted from Liang W. et al. [19 (link)]. Evaluation was performed with an Olympus light microscope BX43, Olympus digital camera DP21 with Olympus cellSens Entry 1.13 software.

Wollman A., Daniel T, & Rosenzweig T. (2019). Sarcopoterium spinosum Inhibited the Development of Non-Alcoholic Steatosis and Steatohepatitis in Mice. Nutrients, 11(12), 3044.

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Tumor Evaluation and Treatment Efficacy

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Three mice per group were randomly selected and sacrificed on day 22 (i.e., two weeks after the last treatment) for tumor collection in 4% formaldehyde in PBS. Formalin-fixed tumors were embedded in paraffin and cut into 4µm-thick sections to confirm model characteristics and to evaluate the treatment efficacy by means of mitotic and apoptotic activity. H&E staining was used for counting mitotic and apoptotic cells in 10 high power fields (HPF) at 400-fold magnification (0.45-mm field diameter). pHH3 and cleaved PARP staining, markers for proliferative and apoptotic activity, were used for counting the number of immune-positive tumor cells in 10 HPF. Additionally, the AXL expression for comparison with the response to EnaV in a given model was evaluated on experimental passage by IHC on isotype control ADC-treated tumors. Whole tumor sections were given an AXL IHC-score based on the overall intensity of staining: 0—negative, 1—weakly, 2—intermediate, and 3—strongly positive. Histological analysis was performed using a CH30 microscope (Olympus, Tokyo, Japan). Pictures were taken using a BX43 light microscope (Olympus).

Van Renterghem B., Wozniak A., Castro P.G., Franken P., Pencheva N., Sciot R, & Schöffski P. (2022). Enapotamab Vedotin, an AXL-Specific Antibody-Drug Conjugate, Demonstrates Antitumor Efficacy in Patient-Derived Xenograft Models of Soft Tissue Sarcoma. International Journal of Molecular Sciences, 23(14), 7493.

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Angiogenesis Assay with JAK2-STAT3 Inhibitor

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Matrigel (50 µl) was spread into 96-well plates at 37°C for 30 min. After H/R treatment for 30 min at 37°C, 100 µl HUVECs (2×10⁵ cell/ml) were added to each well. To confirm the role of JAK2-STAT3 pathway in angiogenesis, JAK2-STAT3 pathway inhibitor AG490 was added. Angiogenesis was observed after 6 h incubation at 37°C under a BX43 light microscope (Olympus Corporation).

Wang Y., Gao H., Cao X., Li Z., Kuang Y., Ji Y, & Li Y. (2022). Role of GADD45A in myocardial ischemia/reperfusion through mediation of the JNK/p38 MAPK and STAT3/VEGF pathways. International Journal of Molecular Medicine, 50(6), 144.

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Histological Analysis of Muscle Tissue

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Muscle sections (0.5 cm × 0.5 cm × 1 cm) were embedded in molds with Tissue-Tek® O.C.T. Compound (Sakaru, Tokyo, Japan), cooled with liquid nitrogen, and sliced into 4 μm sections with a freezing microtome (CM1950, Leica, Wetzlar, Germany). Prepared samples were placed on positively charged microscope slides for H&E and immunofluorescence analyses.
Specimens were stained with H&E and then visualized under an Olympus BX43 light microscope (Olympus, Tokyo, Japan). Three fields of view (0.35 mm × 0.35 mm) were randomly selected from each sample to assess myofiber morphological characteristics.
For immunofluorescence staining, antigens were repaired with antigen retrieval solution (Beyotime, Nanjing, China), treated with 3% hydrogen peroxide, blocked in blocking buffer (5% goat serum, 2% bovine serum albumin, and 0.2% Triton X-100), and incubated with primary antibodies overnight at 4 °C. Slides were rewarmed to approximately 20 °C under ambient conditions and incubated with immunofluorescent secondary antibodies for 2 h. Subsequently, the slides were stained with DAPI and covered with cover slides. Immunofluorescence images were obtained using a DMI4000B inverted fluorescence microscope (Leica). Antibody information is listed in Table S3.

Zhang Y., Li C., Zhou X., Jiang W., Wu P., Liu Y., Ren H., Zhang L., Mi H., Tang J., Zhang R, & Feng L. (2023). Implications of vitamin D for flesh quality of grass carp (Ctenopharyngodon idella): antioxidant ability, nutritional value, sensory quality, and myofiber characteristics. Journal of Animal Science and Biotechnology, 14, 134.

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Histological Assessment of Tumor Proliferation

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Tumors collected for histological assessment were cut into 4-µm sections after formalin-fixation and paraffin embedding. Sections were microscopically evaluated to confirm model characteristics (control tumors) and to evaluate the treatment efficacy in terms of proliferation and apoptosis. Mitotic and apoptotic activity were assessed on H&E-stained sections by counting mitotic and apoptotic figures in 10 high power fields (HPF) at 400-fold magnification (0.45-mm field diameter). pHH3 and cleaved PARP stainings, as markers for proliferation and apoptosis, were assessed by counting the number of positive tumor cells in 10 HPF. Histological analysis was performed blindly for treatment groups using a CH30 microscope (Olympus, Tokio, Japan). Pictures were taken using a BX43 light microscope (Olympus).

Van Renterghem B., Wozniak A., Tarantola L., Casazza A., Wellens J., Nysen M., Vanleeuw U., Lee C.J., Reyns G., Sciot R., Kindt N, & Schöffski P. (2022). Enhanced Antitumor Efficacy of PhAc-ALGP-Dox, an Enzyme-Activated Doxorubicin Prodrug, in a Panel of THOP1-Expressing Patient-Derived Xenografts of Soft Tissue Sarcoma. Biomedicines, 10(4), 862.

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