Imidazole

For the synthesis of surfactant and mesoporous molecular sieves, the following standard compounds were used: imidazole (Shanghai Macklin Biochemical Co., Shanghai, China, ≥99.0%), 1,3-dibromopropane (Shanghai Macklin Biochemical Co., Shanghai, China, ≥99.0%), hexadecyltrimethylammonium bromide (Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China ≥98.0%), bromohexadecane (Shanghai Macklin Biochemical Co., Shanghai, China, 98.0%), acrylonitrile (Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China, ≥99.5%), ethyl acetate (Shanghai Macklin Biochemical Co., Shanghai, China, ≥99.5%), tetraethylorthosilicate (Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China, 98.0%) and crystal (Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China, ≥90.0%), ammonia (Shanghai Macklin Biochemical Co., Shanghai, China, 25%), anhydrous ethanol (Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China, ≥99.5%), isopropanol (Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China, ≥99.5%) and methanol (Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China, ≥99.5%). All experimental reagents were used directly without further purification.

Sphingopyxis sp. USTB-05 used in this study was isolated and identified by us previously [45 (link)]. Trifluoroacetic acids and methanol were purchased from Dikma Technology Inc. (Lake Forest, CA, USA) and used for HPLC analysis. E. coli TOP10 and E. coli BL21 (DE3) were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). The recombinant and host strains were cultured in Luria–Bertani medium (tryptone 10 g, NaCl 10 g, yeast extract 5 g in 1 L H₂O) in a shaker at 200 revolutions per minute (rpm) at 30 °C. The vector pET30a (+), restriction enzymes Sac I and Not I, plasmid mini-prep kit, polymerase chain reaction (PCR) kit and Ni-NTA Sefinose (TM) Resin Kit were also obtained from Sangon Biotech Co., Ltd. (Shanghai, China). Imidazole was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). NOD was purchased from Absin Bioscience Inc. (Shanghai, China) and stored at −20 °C. The purity of all other chemicals was no less than 99.7%.

HPLC-grade, LC-MS-grade acetonitrile (ACN) and methanol (MeOH) were purchased from Dikma Co., Ltd. (Massier Lane, Foothill Ranch, CA, USA). Normal saline was obtained from Qidu Pharmaceutical Co., Ltd. (Shandong, China). Hydrogen trifluoride triethylamine, tetrahydrofuran (THF), tertiary butyl dimethyl chlorosilane (TBSCl), dimethylaminopyridine (DMAP), imidazole, triethylamine, and ammonium acetate were obtained from Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Formic acid, phosphoric acid, N,N′-carbonyldiimidazole (CDI), dicyclohexylcarbodiimide (DCC), dimethyl formamide (DMF), and isobutyric acid were purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). GS441524 (GS441, MW291.3) and 6,7-dimethyl-2,3-bis(2-pyridyl) quinoxaline (QX, MW312.4) were obtained from APExBIO Co., Ltd. (Houston, TX, USA) and JiSiEnBei International Business Co., Ltd. (Hong Kong, China), respectively.