Eclipse ni u

For RIN-14B cells, for taking green fluorescence photography with the ROS kit, cells were incubated with 15 mM DCFH-DA work solution for 30 min at 37 °C, washed thrice with PBS and photographed with a fluorescence microscope (Nikon Eclipse Ni-U) within 15 min.
When it comes to orange fluorescence photography with DHE kit, cells were incubated with 20 mM DHE work solution for 30 min at 37 °C, washed twice with PBS and photographed with a fluorescence microscope (Nikon Eclipse Ni-U) within 15 min.
For larval zebrafish, after incubation with 15 μM DCFH-DA work solution (diluted with embryo water) for 30 min at 27 °C, 6 larval were transferred into 200 mL embryo water for 3 min to wash the DCFH-DA physically absorbed on the body surface, then stained cutaneous cells with DAPI (Sigma, USA) to highlight the green fluorescence from the cells in which ROS were accumulated. Larval zebrafish were kept into the 3.5% sodium carboxymethylcellulose (CMC-Na) on a glass slide for fixation. The images were captured immediately using a fluorescence stereoscope (Nikon Eclipse Ni-U), and all the captures were taken in the dark room with the same parameters (exposure time, ISO and aperture) for comparison with different groups.

Cyanobacterial colonies and cells of wild-type and vp28-mOrange mutant of S. elongatus were observed under a fluorescence microscope (Nikon Eclipse NI-U, Japan) using mOrange filters (ET530/30x; ET575/40m). The cyanobacterial colonies on a solid medium were observed under a 4-fold objective lens directly. Cells in liquid medium were dropped onto a glass slide and observed under a 20-fold objective lens directly. Exposure times were 350 ms and 1500 ms.

The experimental equipment were a paraffin microtome (RM2235, Leica Microsystems, Inc.), a microtome (YT-7FB, Leica Microsystems, Inc.), an automatic immunohistochemistry machine (Benchmark XT, Ventana Medical Systems, Inc.), and an optical microscope (ECLIPSE Ni-U, Nikon Corporation, Japan).