Gsh glo glutathione assay
The GSH-Glo Glutathione Assay is a luminescent-based assay that quantifies the amount of glutathione in biological samples. It utilizes a luminogenic substrate that reacts with glutathione to generate a luminescent signal, which is proportional to the amount of glutathione present in the sample.
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101 protocols using gsh glo glutathione assay
Glutathione Concentration Quantification
Hydrogen Peroxide and Glutathione Assays
Quantifying Cellular Glutathione Levels
Assessing Cellular Redox Status
Intracellular Glutathione Quantification
Quantification of Cellular Glutathione Levels
Glutathione Assay Protocol
Glutathione Measurement via Luminescence
Changes in GSH levels were monitored with the luminescence-based GSH-Glo glutathione assay (Promega, Madison, WI, USA) according to the manufacturer’s instructions. Essentially, the assay couples two chemical reactions. The first reaction is catalyzed by glutathione-S-transferase and converts a GSH probe, luciferin-NT, into luciferin. In the second reaction, catalyzed by firefly luciferase, the amount of luciferin produced is detected as a luminescent signal. The overall intensity of the signal is proportional to the GSH amount present in each sample. At the end of exposure to copper and myricetin, the medium was removed, and 100 μL of the GSH-Glo reagent was added to each well. The plates were incubated for 30 min at RT in the dark. Thereafter, 100 μL of the luciferin detection reagent was added, mixed briefly, and incubated for a further 15 min. Emitted luminescence was quantified by using a Fluoroskan Ascent FL luminometer (Thermo Fisher Scientific, Waltham, MA, USA).
Oxidative Stress Measurements in Tumorspheres
Glutathione Quantification in Infected Cells
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