Luciferase-expressing CT26 murine colon carcinoma cells (ATCC CRL-2638) were used in this study. Cells were maintained in Roswell Park Memorial Institute (RPMI) 1640 Medium (Pan Biotech, Germany) containing 10% fetal bovine serum, 1% glutamine, and 1% penicillin–streptomycin (all Sigma, Germany). Sub-culturing was performed every 2–3 days. Cells were kept at 37 °C, 95% humidity, and 5% CO2 in a cell culture incubator (Binder, Germany).
1640 medium
Manufactured by PAN Biotech
Sourced in Germany
1640 medium is a cell culture medium formulated for the in vitro cultivation of a variety of cell types. It is a chemically defined, serum-free medium that provides the necessary nutrients and components to support cell growth and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Merck Group (2 mentions)
Cell culture incubator, by Binder (2 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Penicillin streptomycin, by Corning (1 mentions)
24 well plate, by Greiner (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
Glutamine, by Corning (1 mentions)
Puromycin, by STEMCELL (1 mentions)
Mem medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by PAN Biotech (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Antibiotic antimycotic solution, by Merck Group (1 mentions)
5 protocols using 1640 medium
1
Culturing Murine Colon Carcinoma Cells
2
Cultivation of Urothelial Carcinoma Cell Lines
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The human urothelial carcinoma cell lines T24, RT-112, CLS-439 (abbreviated here as CLS; CLS Cell lines Service, Germany), and SCaBER (squamous differentiation) (all others: ATCC, USA) were cultivated in Roswell Park Memorial Institute (RPMI) 1640 medium (Pan Biotech, Germany) supplemented with 10 % fetal bovine serum, 1 % penicillin/streptomycin, and 1 % glutamine (all Corning, Germany). Cells were kept under standard culture conditions at 37 °C, 5 % CO2, and 95 % humidity in a cell culture incubator (Binder, Germany). Twenty-four hours before experiments, 5x104 cells in 500 µl fully-supplemented medium were seeded in 24-well plates (Greiner Bio-One, Germany). After gas plasma exposure, evaporated liquid was compensated for by adding predetermined amounts of double-distilled H2O.
3
Murine Melanoma Cell Culture
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Ova‐expressing murine melanoma cells (B16F10‐Ova) were a kind gift of Karl Sebastian Lang (Institute of Immunology, University Hospital Essen, Germany). Cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (PanBioTech, Germany) containing 10% fetal bovine serum, 2% glutamine, 1% penicillin/streptomycin (all Sigma, Germany), and 0.5 µg mL−1 puromycin (StemCell Technologies, Germany). Cells were grown at 37 °C, 95% humidity, and 5% CO2, and subcultured three times a week.
4
Renal Clear Cell Carcinoma Tissue and Cell Line Study
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This study collected carcinoma and paired adjacent tissues from six patients undergoing renal clear cell carcinoma surgery at Lanzhou University Second Hospital from January 2020 to June 2020.The ethics of tissue specimens was reviewed and endorsed by the Ethics Committee of the Lanzhou University Second Hospital, and each patient enrolled in the group was given information and signed a consent form(Approval Number: 2023 A-268).The cell lines (HK2,7860, CAKI-1, and A498 cells) used in this study were all offered by the Gansu Provincial Key Laboratory of Urological Diseases.The HK2 cells were cultured using DMEM / F12 medium (Gibco) supplemented with 10% fetal bovine serum (PAN-Biotech GmbH),7860, CAKI cells were cultured using 1640 medium (basalmedia) supplemented with 10% fetal bovine serum (PAN-Biotech GmbH),the A498 cells were cultured using MEM medium (Gibco) supplemented with 10% fetal bovine serum (PAN-Biotech GmbH),all cells were cultured in 5%CO2 at 37℃.
5
Environmental Sampling for COVID-19 Decontamination
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Environmental samples were collected when the infection was ongoing at 7 dpi, at 14 dpi (before decontamination) and at 15 dpi (24 h after decontamination). Two millilitres of Roswell Park Memorial Institute 1640 medium (PAN Biotech, Aidenbach, Germany) as a growth medium (GM) supplemented by 1% Antibiotic-Antimycotic Solution (Sigma-Aldrich, St. Louis, MO, USA) and 10% foetal bovine serum (GIBCO, Thermo Fisher Scientific, Waltham, MA, USA) was placed into a plastic tube. Designated spots were thoroughly scrubbed by swabs and immersed in the medium. Swabs from the floor (five spots), feeder (one spot), drinker (one spot), a rubber mat (one spot), a wall at a height of about 30 cm (one spot), the HEPA filter cover (one spot), and the doors at a height of about 120 cm (one spot) were collected in triplicate. Samples were aliquoted and stored at -80°C for real-time PCR and in vitro analyses.
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