Ethyl p aminobenzoate benzocaine
Ethyl p-aminobenzoate, also known as benzocaine, is a laboratory reagent used as a local anesthetic. It is a colorless crystalline solid with a characteristic odor. Benzocaine is primarily utilized as an active ingredient in topical anesthetic formulations and has various applications in the pharmaceutical and medical industries.
Lab products found in correlation
10 protocols using ethyl p aminobenzoate benzocaine
Axolotl Forelimb Regeneration Protocol
Fish Welfare in Experimental Procedures
Ethical Handling of Eels and Larvae
Animal Welfare in Fish Research
CRISPR-Cas9 Mediated Genome Editing in Newts
Single-cell fertilized eggs were obtained by either natural or induced breeding and injected according to previously published protocols with modifications60 (link), 61 (link). Briefly, 500 pg Cas9 RNA and 100 pg gRNA were mixed into 5 nl and injected into freshly laid single-cell-stage embryos. The animals were raised according to60 (link). The screening of F0 animals was done by both genotyping (see above) and phenotype characterization, including pigmentation analysis and immunohistochemistry of limbs/tails, according to39 (link), 40 (link). The Pax7−/− and Tyr−/− F1 animals were produced by crossing between adult F0 animals. The larvae or post-metamorphic newts were anaesthetized within 0.01% or 0.1% ethyl-p-aminobenzoate (benzocaine; Sigma) prior to imaging and tissue collection. Newts utilized for this study were processed according to Swedish Board of Agriculture animal ethical regulations (N429/12).
Eel Anaesthesia and Handling Protocol
All efforts were made to minimize animal handling and stress. Permission to use 14C labelled FA was obtained by The Danish Health Board, The State Institute of Radiation Protection.
Anesthesia of Axolotl Specimens
Experimental Maturation of Female European Eels
Female European eels used in this study were at the pre-pubertal stage prior to experiments. As eels undergo a natural fasting period from the pre-pubertal silver stage to the end of the sexual maturation, they were not fed during treatment. All eels were anesthetized using ethyl p-aminobenzoate (benzocaine; Sigma-Aldrich, Germany), before tagging, handling and sacrifice. All efforts were made to minimize animal handling and stress.
Eel Anaesthesia and Handling Protocol
All efforts were made to minimize animal handling and stress. Permission to use 14C labelled FA was obtained by The Danish Health Board, The State Institute of Radiation Protection.
Tissue-specific immune gene expression in European eel
The eels were euthanized by submersion in an aqueous solution of ethyl p-aminobenzoate (benzocaine) at 20 mg L -1 (Sigma-Aldrich, Missouri, USA) and organ tissue samples dissected from hind-gut, gills, head kidney, kidney, liver, skin, spleen, whole brain, heart, and muscle. Samples were stored in RNA-later at -80°C until further use. For further processing see 2.4.
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