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Duoset elisa mouse il 12p70

Manufactured by R&D Systems

The DuoSet ELISA Mouse IL-12p70 is a laboratory reagent kit designed to measure the concentration of mouse interleukin-12 p70 (IL-12p70) in biological samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the target analyte.

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2 protocols using duoset elisa mouse il 12p70

1

Investigating ItaCORM Modulation of BMDC Cytokines

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BMDCs were stimulated with LPS and co-incubated with the respective ItaCORMs or control substances as indicated in the figure legend. After 24 h, supernatants of each culture condition were collected and assayed for IL-12p70 and IL-23 levels. ELISA was performed using DuoSet ELISA Mouse IL-12p70, DuoSet ELISA Mouse IL-23 and DuoSet Ancillary Reagent Kit 2 (R&D Systems).
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2

Murine Cytokine ELISA Quantification

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Murine IFN-γ, TNF-α, and IL-12 levels were quantified in the supernatant of cell cultures or the mouse sera using the ELISA kit from the R&D system (DuoSet ELISA mouse IL-12 p70, TNF-α, or IFN-γ). The amount of each cytokine was calculated based on a calibration curve prepared at the same time with the samples.
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