The patient was placed in prone position after general anaesthesia. The first scan was performed by O-arm (Medtronic, Inc., Dublin, Ireland ) to acquire three-dimensional images. Then a 3 cm incision was made lateral to the midline on the decompression side. Through the incision, dilator was placed and fixed. An operating microscope (Carl Zeiss, Inc., Oberkochen, Germany ) was used during laminectomy and discectomy. The facet joints and the ligamentum flavum were removed by oesteotome and laminectomy rongeur, and the traversing nerve roots, exiting nerve roots and the lateral edge of the dura were exposed and decompressed. The posterolateral annulus was incised, and discectomy was acted completely. After confirming the spacer size, a polyetheretherketone (PEEK) cage filled with autologous cancellous bone was inserted accurately. The homolateral pedicle screw implantation was performed through the decompression incision. Posterior fixation on the contralateral side was done using percutaneous pedicle screws through two 1 cm incisions. A bended rod was place to connect the pedicle screws, and then, the intervertebral space was moderately compressed to make sure the cage was very solid. O-arm scan was acted again to make sure the position of the pedical screws. Then, we sutured incisions, and a drainage tube was placed underneath the fascia.
An operating microscope
Manufactured by Zeiss
Sourced in Germany, United Kingdom
The Zeiss operating microscope is a versatile and highly precision instrument designed for use in medical procedures. It features high-quality optics and advanced illumination systems to provide a clear and detailed view of the surgical site. The microscope's core function is to enhance the visibility and accuracy of medical professionals during delicate procedures.
Automatically generated - may contain errors
Lab products found in correlation
O arm, by Medtronic (1 mentions)
Ketamine, by Zoetis (1 mentions)
Tropicamide, by Bausch & Lomb (1 mentions)
Dormitor, by Pfizer (1 mentions)
Viscotears, by Novartis (1 mentions)
Antisedan, by Pfizer (1 mentions)
Medetomidine hydrochloride, by Pfizer (1 mentions)
Atipamezole hydrochloride, by Pfizer (1 mentions)
3 protocols using an operating microscope
1
Posterior Lumbar Decompression and Fusion
2
Subretinal Cell Injection in Rd1/Nude Mice
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All surfaces were wiped with 70% ethanol prior to surgery. 3 month old rd1/Foxn1nu mice were anesthetized by intraperitoneal injection (10mL/Kg) of a mixture of a mixture of Dormitor (1 mg/ml medetomidine hydrochloride; Pfizer Pharmaceuticals), ketamine (100 mg/ml; Fort Dodge Animal Health), and sterile water in the ratio 5:3:42. Tropicamide (1%; Bausch & Lomb) was used to dilate the pupils and topical anesthetic was applied (Tetracaine). Eyes were kept moist by using Viscotears (Novartis Pharmaceuticals UK Ltd). Surgery was performed under direct visual control using an operating microscope (Zeiss). A sterile 34-gauge hypodermic needle was used to make a small puncture to the anterior chamber to relieve pressure in the orbit. The same needle was used to make a new transcleral entry in the posterior orbit and slowly inject 1.5μl of cell suspension into the sub-retinal space. The same region of the eye was targeted for all injections. Significant care was taken not to rupture the very thin remaining neural retina and the needle was left in place for ∼20 s to allow for re-equilibration of intraocular pressure before slowly withdrawing. Anaesthesia was then reversed using same amount of Antisedan, 10mL/Kg, (atipamezole hydrochloride 0.10 mg/ml, Pfizer Pharmaceuticals, Kent UK), with the mice placed on heat mats until fully recovered.
3
Intravitreal Antibody Injection in Mice
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Mice were anesthetized and pupils dilated using the same methods as above. With the aid of an operating microscope (Zeiss), 2 μL 20 mg/mL antibody (in PBS) was injected intravitreally with a 5-μL Hamilton syringe (Esslabs, Hadleigh, UK) fitted with a 34G needle (Esslabs). For each mouse, anti-VEGF scFv or anti-VEGF IgG1 (n = 6 or n = 5, respectively) was injected into one eye and an equal volume of PBS in the other (control) eye.
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