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Oddessy system

Manufactured by LI COR
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Sourced in United States

The Odyssey system is a high-performance imaging system designed for a variety of applications in life science research. It utilizes infrared and near-infrared fluorescence detection to provide sensitive and quantitative analysis of protein, nucleic acid, and small molecule samples. The system is capable of detecting and analyzing multiple targets simultaneously within a single experiment.

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Lab products found in correlation

Cycloheximide (chx), by Selleck Chemicals (1 mentions) Sin3a, by Abcam (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Li-Cor Oddessy Imaging System Oddessy fluorescent imaging system

2 protocols using oddessy system

1

Western Blotting Antibody Validation

Cited 1 time
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Methods for Western blotting was described elsewhere16 (link). Polyclonal rabbit antibody for Fli-1 were obtained from Abcam (Abcam, Cambridge, UK); ERK and phospho-ERK from Cell Signalling Technology (CST, Danvers, MA01923), β-actin, β-Tubulin, BCL-2 from Proto-Technology (Protein-Tech, Bucuresti, Romania) and goat-anti-mouse, and goat anti-rabbit HRP-conjugated (Promega). Antibody dilution was according to the manufacturer instructions. Imaging of proteins was performed using Oddessy system (Li-Cor biosciences, Lincoln, USA). In experiments described in Fig. 7a, we used ECL detection system for western blotting, as described9 (link).
Liu T., Xia L., Yao Y., Yan C., Fan Y., Gajendran B., Yang J., Li Y.J., Chen J., Filmus J., Spaner D.E., Zacksenhaus E., Hao X, & Ben-David Y. (2019). Identification of diterpenoid compounds that interfere with Fli-1 DNA binding to suppress leukemogenesis. Cell Death & Disease, 10(2), 117.
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2

Western Blot Analysis of Protein Signaling

Cited 2 times
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Western blots performed according to published methods43 (link),44 (link). Polyclonal rabbit antibodies for FLI1, BCL2, SIRT3, SIN3A, cMYC obtained from Abcam, Cambridge, UK; ERK, phospho-ERK, AKT, phospho-AKT, GAPDH, SAP18 antibodies obtained from Cell Signaling Technology (CST), Danvers, MA01923; goat-anti-mouse and goat anti-rabbit HRP-conjugated antibodies obtained from Promega, Madison, Wisconsin, USA. Antibody dilution conducted according to the manufacturer’s instructions. The Oddessy system (Li-Cor Biosciences, Lincoln, USA) used for protein detection. Proteasome (MG132) and transcription (Actinomycin D) and translation (Cycloheximide) inhibitors were obtained from Selleckchem.com and used in some experiments.
Gajendran B., Varier K.M., Liu W., Wang C., Sample K.M., Zacksenhaus E., Juiwei C., Huang L., Hao X, & Ben-David Y. (2020). A C21-steroidal derivative suppresses T-cell lymphoma in mice by inhibiting SIRT3 via SAP18-SIN3. Communications Biology, 3, 732.
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