Ceftazidime hydrate and seleno-L-methionine (Sigma-Aldrich, St. Louis, MO) were dissolved in water, sterile filtered, and frozen in aliquots of 10 mg/ml and 50 mM solutions, respectively. IFN-γ (PeproTech, Rocky Hill, NJ) was reconstituted according to the manufacturer’s instructions, aliquoted (100 μg/ml) and frozen at -80°C until use. Other reagents included kanamycin monosulfate (MP Biomedicals, Solon, OH) and 0.4% Trypan Blue solution (Mediatech, Manassas, VA).
Kanamycin monosulfate
Manufactured by MP Biomedicals
Kanamycin monosulfate is a broad-spectrum antibiotic used in research and laboratory settings. It is a salt of kanamycin, a bactericidal agent effective against various Gram-positive and Gram-negative bacteria. Kanamycin monosulfate is commonly used as a selective agent in bacterial cell culture and genetic engineering applications.
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Lab products found in correlation
2 protocols using kanamycin monosulfate
1
Preparing Ceftazidime and Seleno-L-methionine
2
Preparation of Antimicrobial Compound Solutions
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Buffers and salts were of American Chemical Society reagent grade or higher and were purchased commercially, from Carl Roth GmbH and Sigma-Aldrich, biological materials and growth media were purchased from Sigma-Aldrich, Scharlab S.L. and Fischer Scientific. Antibiotics trimethoprim (Sigma-Aldrich), ceftazidime (ceftazidime penta hydrate, Thermo Scientific) and kanamycin (kanamycin monosulfate, MP biomedicals) were dissolved in DMSO stored in -20 °C, apart from CIP, which was used from a 3 M aqueous solution containing 0.01% AcOH. All test compounds were used from 10 mM DMSO stock solutions made from the freeze dried powder and stored at -20 °C.
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