Sodium hydroxide (NaOH), hydrochloric acid (HCl), trichloroacetic acid (TCA) and acetic acid were from Fisher Scientific (Dublin, Ireland). Kjeldahl tablets, sulfuric acid (>98%, H2SO4), boric acid, trifluoroacetic acid (TFA), Trizma® base, dimethyl sulfoxide (DMSO), 2-mercaptoethanol, methanol, protein markers (6.5–200 kDa), Sudan III, 2,4,6-trinitrobenzenesulfonic acid (TNBS), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), porcine pepsin (2500 U/mg), porcine pancreatic lipase (50 mU/mL), 4-methylumbelliferyl oleate (4-MUO) and Orlistat® were from Sigma-Aldrich (Dublin, Ireland). Hexane, MS-grade water and acetonitrile (ACN) were from Honeywell International Inc. (Dublin, Ireland). Comassie R, Laemmli buffer and Mini-Protean TGX 4–20% pre-cast polyacrylamide gels were from Bio-Rad Laboratories Inc. (Hercules, CA, USA). Sodium dodecyl sulfate (SDS) was from National Diagnostics (Atlanta, GA, USA). Corolase PP® (4.4 U/mg) was from AB Enzymes GmbH (Darmstadt, Germany). Cellulose acetate filters were from Millipore (Carrigtwohill, Ireland) and polytetrafluoroethylene syringe filters were from VWR (Dublin, Ireland).
Mini protean tgx 4 20 pre cast polyacrylamide gels
Manufactured by Bio-Rad
Sourced in United States
The Mini-Protean TGX 4–20% pre-cast polyacrylamide gels are laboratory equipment designed for protein electrophoresis. They provide a pre-cast polyacrylamide gel matrix with a gradient range of 4% to 20% for the separation of proteins based on their molecular weight.
Automatically generated - may contain errors
Lab products found in correlation
Acetic acid, by Thermo Fisher Scientific (2 mentions)
Kjeldahl tablets, by Merck Group (2 mentions)
Sudan 3, by Merck Group (2 mentions)
Comassie r, by Bio-Rad (2 mentions)
Laemmli buffer, by Bio-Rad (2 mentions)
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
2 mercaptoethanol, by Merck Group (2 mentions)
Boric acid, by Merck Group (2 mentions)
Protein marker, by Merck Group (1 mentions)
Porcine pancreatic lipase, by Merck Group (1 mentions)
Cellulose acetate filter, by Merck Group (1 mentions)
Orlistat, by Merck Group (1 mentions)
2 4 6 trinitrobenzenesulfonic acid, by Merck Group (1 mentions)
4 methylumbelliferyl oleate 4 muo, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Trizma base, by Merck Group (1 mentions)
Porcine pepsin, by Merck Group (1 mentions)
Acetonitrile acn, by Honeywell (1 mentions)
2 protocols using mini protean tgx 4 20 pre cast polyacrylamide gels
1
Lipase and Pepsin Activity Assay
2
Comprehensive Protein Characterization Protocol
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SPI, PPI and RPI from Pulsin Ltd. (Gloucester, UK) were purchased at a local health food store. MPC85 (85% (w/w) protein) was from a commercial supplier. The details of the composition of plant protein samples and MPC are provided in supplementary data (Table S1). Sodium hydroxide, acetic acid and trinitrobenzenesulfonic acid (TNBS) were from Fisher Scientific (Dublin, Ireland). The hydrochloric acid and trichloro acetic acid (TCA) was from VWR (Dublin, Ireland). The Kjeldahl tablets, sulfuric acid (>98%), boric acid, 2-mercaptoethanol, methanol, protein molecular weight (MW) markers (6.5-200 kDa), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), pepsin (1616 U/mg), Sudan III, ninhydrin reagent (2% (w/v) solution), trifluoroacetic acid (TFA) and Trolox standard were from Sigma-Aldrich (Dublin, Ireland). The hexane and acetonitrile (ACN) were from Honeywell International Inc. (Dublin, Ireland). Comassie R, Laemmli buffer and Mini-Protean TGX (4-20%) pre-cast polyacrylamide gels were from Bio-Rad Laboratories Inc. (CA, USA). The sodium dodecyl sulphate (SDS) was from National Diagnostics (GA, USA). Corolase PP ® (4.4 U/mg) was from AB Enzymes GmbH (Darmstadt, Germany). PDCAAS analysis kits were purchased from Megazyme (Bray, Ireland).
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