Dermatophagoides farinae

Manufactured by Stallergenes Greer

Sourced in United States

Dermatophagoides farinae is a species of dust mite that is commonly used as a laboratory specimen. It is a small, microscopic arthropod that is a member of the Acaridae family. The Dermatophagoides farinae mite is often utilized in research and testing related to allergens and immune responses.

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Lab products found in correlation

Dermatophagoides pteronyssinus, by Stallergenes Greer (3 mentions) Resiniferatoxin, by Merck Group (2 mentions) Staphylococcal enterotoxin b, by Merck Group (2 mentions) Anti claudin 1, by Abcam (2 mentions) Alexa 594 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Alexa488 conjugated avidin, by Thermo Fisher Scientific (2 mentions) Celltrace cfse cell proliferation kit, by BD (2 mentions) Anti cd4 apc, by BD (2 mentions) Anti il 4 pe, by BD (2 mentions) Anti il 5 pe, by BD (2 mentions) Anti il 13 pe, by BD (2 mentions) Anti ifn γ pe, by BD (2 mentions) Purified na le rat anti mouse cd124 mil4r m1, by BD (2 mentions) Control istoype r35 95, by BD (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Saponin, by Merck Group (2 mentions) Capsaicin, by Merck Group (2 mentions) Sodium citrate, by Merck Group (2 mentions) Ovalbumin (ova), by Merck Group (1 mentions) Hnecs, by American Type Culture Collection (1 mentions)

10 protocols using dermatophagoides farinae

Characterization of Skin Barrier Function

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Sodium citrate, Bovine Serum Albumin (BSA), DMSO, saponin, Capsaicin,
Resiniferatoxin and Staphylococcal Enterotoxin B (SEB) were from Sigma-Aldrich.
HDM extracts of the strain Dermatophagoides farinae were
purchased from Greer Laboratories. The following antibodies were obtained from
Covance: anti-Keratin (K) 14, anti-K6, anti-K10, anti-loricrin and
anti-filaggrin. Anti-Claudin 1 was from Abcam. Alexa594-conjugated goat
anti-rabbit IgG, Alexa488-conjugated avidin and DAPI were from Life Technologies
Invitrogen. The following reagent and antibodies were from eBioscience
Thermofisher Scientific: CellTrace™ CFSE Cell Proliferation Kit,
anti-CD4-APC, anti-IL4-PE, anti-IL-5-PE, anti-IL-13-PE, and anti-IFNγ-PE.
Purified NA/LE Rat Anti-Mouse CD124 (mIL4R-M1) or control Istoype (R35-95) were
from BD Bioscience. MYD88 inhibitory peptide (i.e., a cell permeant peptide
sequence that selectively blocks MYD88 homodimerization) and a control peptide
(i.e., an inactive and cell permeant truncated form of the MYD88 inhibitory
peptide) were from Novus. Alternaria alternata (A. alternata),
Dermatophagoides pteronyssinus (D. pteronyssinus), ragweed and
German Coackroach were all from Stallergenes Greer.

Serhan N., Basso L., Sibilano R., Petitfils C., Meixiong J., Bonnart C., Reber L.L., Marichal T., Starkl P., Cenac N., Dong X., Tsai M., Galli S.J, & Gaudenzio N. (2019). House dust mites activate nociceptor-mast cell clusters to drive type 2 skin inflammation. Nature immunology, 20(11), 1435-1443.

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Allergic Sensitization Evaluation in Infants

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Rhinitis was defined as having symptoms of sneezing, runny and/or blocked nose that lasted for at least four weeks in single or multiple episodes (each episode lasting at least two weeks) This definition was based on the Allergic Rhinitis and its Impact on Asthma (ARIA) guidelines [4 (link)]. Wheeze was defined as presence of wheeze symptoms (noisy breathing with a high-pitched, whistling sound heard from the chest, not the mouth). Eczema was defined as physician-diagnosed eczema in the first 18 months. At the 18 month visit, subjects were assessed via skin prick test (SPT) for the presence of allergic sensitization to a panel of food (milk, peanut, egg) and house dust mites allergens (Dermatophagoides pteronyssinus, Dermatophagoides farinae) from Greer Laboratories (Lenoir, NC, USA) and Blomia tropicalis (extract obtained from in-house laboratory) [18 (link)]. Wheal size of at least 3mm larger than the negative control (saline) was inferred as a positive reaction.

Huy Ta L.D., Yap G.C., Tay C.J., Lim A.S., Huang C.H., Chu C.W., De Sessions P.F., Shek L.P., Goh A., Van Bever H.P., Teoh O.H., Soh J.Y., Thomas B., Ramamurthy M.B., Goh D.Y., Lay C., Soh S.E., Chan Y.H., Saw S.M., Kwek K., Chong Y.S., Godfrey K.M., Hibberd M.L, & Lee B.W. (2018). Establishment of the Nasal Microbiota in the first 18 Months of Life – Correlation with Early Onset Rhinitis and Wheezing. The Journal of allergy and clinical immunology, 142(1), 86-95.

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Allergic Airway Inflammation Asthma Treatment

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Example 3

Splenocytes were isolated from DO11.10 mice and ˜1×10⁶CD4T cells were transferred to pure BALB/c mice by intravenous injection. For 3 days from the next day, 100 μg of a mixture of HDM (Dermatophagoidesfarinae 50 μg, Dermatophagoidespteronyssinus 50 μg, Greer Laboratories, Inc., USA) and OVA (Sigma-Aldrich Co., USA) was administered intranasally to the mice. From day 2 after administration, the mice were treated with 200 μg of each of the inventive compounds or PBS (for control mice) three times daily through the intraperitoneal route. After the mice were euthanized on day 11, the numbers of total cells and eosinophils in the airways were checked. The treatment schedule with the Formula 5 (KB 1517) and Formula 10 (KB 1518) (200 μg each) in house dust mite (HDM)-induced allergic airway inflammation mouse models is shown in FIG. 8. The results are shown in FIGS. 4 and 5.

As can be seen from FIGS. 4 and 5, the numbers of total cells and eosinophils in the airways of the mice treated with the inventive compounds of Formula 5 (KB-1517) and Formula 10 (KB-1518) (200 μg each) were significantly reduced than those in the untreated controls. These results lead to the conclusion that the inventive quinolinone derivatives can be used to fundamentally prevent and treat allergic diseases such as asthma atopic dermatitis.

US11168094B2. Quinolinone derivative and pharmaceutical composition for preventing or treating allergic diseases such as asthma or atopic dermatitis including the quinolinone derivative as active ingredient (2021-11-09). Azcuris Co., Ltd. [KR]. Inventors: Youngjoo Byun [KR], Young Ho Jeon [KR], Kiho Lee [KR], Ki Yong Lee [KR], Yong Woo Jung [KR], Sang-Hyun Son [KR].

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Multitarget Allergic Rhinitis Model

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Mice, under light isoflurane anesthesia, were treated intranasally (i.n.) with a 30 µL volume (15 µL into each nostril) of DRA solution containing 5 µg of dust mite (extracts of Dermatophagoides farinae, Greer Laboratories, NC, USA), 50 µg of ragweed (extracts of Ambrosia artemisiifolia, Greer Lab) and 5 µg of Aspergillus (extracts of Aspergillus fumigatus, Greer Lab), twice a week for 8 weeks. The control group received 30 µL (15 µL into each nostril) of saline i.n. using the same protocol. Among the animals undergoing DRA challenge a group was treated with 100 μg/Kg budesonide i.n. 6 day/week for 4 weeks starting at the end of week 4. Eight mice were used for every group and the experiment was replicated three times.

Stellari F., Sala A., Ruscitti F., Carnini C., Mirandola P., Vitale M., Civelli M, & Villetti G. (2015). Monitoring inflammation and airway remodeling by fluorescence molecular tomography in a chronic asthma model. Journal of Translational Medicine, 13, 336.

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Allergic Airway Inflammation in Mice

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Eight-week-old mice were sensitized subcutaneously with HDM (100 μg per mouse; Dermatophagoides farinae, Greer Laboratories) in complete Freund's adjuvant (CFA) on day 0 and then intranasally challenged with HDM (100 μg per mouse) on day 14. BAL cell counting and tissue collection were performed 24 h after the last HDM challenge.

Herjan T., Xiao J, & Dziendziel Kolanek M. (2022). RNA-Binding Protein HuR Promotes Airway Inflammation in a House Dust Mite-Induced Allergic Asthma Model. Journal of Interferon & Cytokine Research, 42(1), 29-38.

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Characterization of Skin Barrier Function

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HNECs and ILC2s Coculture Protocol

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Human nasal epithelial cell line (HNECs) was obtained (ATCC, Rockville, MD, USA) and cultured in bronchial epithelial cell basal medium (BEBM, Lonza, Walkersville, MD, USA) at 37 °C in a 5% CO₂-humidified chamber. After the cells were differentiated, they were incubated with 25 μg/mL Dermatophagoides farinae (Greer Laboratories, Lenoir, NC, USA) under different transfection status as described below. After 24 h of treatment, culture supernatants were collected and centrifuged at 500 rpm for 5 min to remove cell debris for subsequent analysis.
HNECs and ILC2s were co-cultured in Transwell chamber. The stimulating cytokines from R&D system company included IL-25 (10 ng/ml), IL-33 (10 ng/ml), TSLP (10 ng/ml), IL-2 (50 ng/ml), anti-TSLP (10 ng/ml), 100 ng/ml AG490 (JAK inhibitor). The treatment lasted 24 h at the presence of Dermatophagoides farinae.

Luo X., Zeng Q., Yan S., Liu W, & Luo R. (2020). MicroRNA-375-mediated regulation of ILC2 cells through TSLP in allergic rhinitis. The World Allergy Organization Journal, 13(8), 100451.

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Evaluating Nanoparticle Uptake in A549 Cells

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A549 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin (Gibco) at 37°C in a 5% CO₂ incubator. In experiments to determine the involvement of NPs uptake, the cells were incubated with 50 μg/mL house dust mite (HDM, Dermatophagoides farinae, Greer Laboratories) for 24 h. Then, A549 cells were treated with naked Cou6 NPs or PM@Cou6 NPs for 2 h. After the cells were washed two times, A549 cells treated with or without HDM were imaged by fluorescence microscopy (Olympus IX70 Inverted Microscope).

Jin H., Li J., Zhang M., Luo R., Lu P., Zhang W., Zhang J., Pi J., Zheng W., Mai Z., Ding X., Liu X., Ouyang S, & Huang G. (2021). Berberine-Loaded Biomimetic Nanoparticles Attenuate Inflammation of Experimental Allergic Asthma via Enhancing IL-12 Expression. Frontiers in Pharmacology, 12, 724525.

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Allergen Extraction and Preparation

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Ovalbumin from chicken egg white was from Sigma-Aldrich (St. Louis, MO, USA). The allergens comprising Alternaria alternata, Aspergillus fumigatus, and Dermatophagoides farinae (House dust mite) were purchased from Greer laboratories, Lenoir, NC, USA. DPBS (Cat# 14190-144) and protease and phosphatase inhibitor (PPI) cocktail (Cat# 1861284) were purchased from Thermo Fisher Scientific, Waltham, MA, USA.

Borkar N.A., Ambhore N.S., Balraj P., Ramakrishnan Y.S, & Sathish V. (2023). Kisspeptin regulates airway hyperresponsiveness and remodeling in a mouse model of asthma. The Journal of pathology, 260(3).

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Childhood Opportunity Index and Allergic Sensitization

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At enrollment, caregivers completed demographic questionnaires and medical history questionnaires. ZIP code features were obtained from the United States Census Bureau QuickFacts and expressed in 2020 dollars, where applicable. 9 Residential addresses were geocoded to census tracts and the Childhood Opportunity Index 2.0 was joined to patient data using the census tract GEOID as described previously. 10, 11 Higher Childhood Opportunity Index values reflect better opportunity. 12 Preschool participants also submitted blood samples for blood eosinophil counts and total and specific serum IgE (Children's Healthcare of Atlanta, Atlanta, Georgia). Specific IgE testing was performed with twelve extracts: Dermatophagoides farinae, Dermatophagoides pteronyssinus, dog dander, cat dander, Blatella germanica, Alternaria tenuis, Aspergillus fumagatis, oak tree, pecan tree, Bermuda grass, Johnson grass, and common ragweed (Ambrosia artemisiifolia) (Greer ® Laboratories, Lenoir, North Carolina). Children were considered sensitized if specific IgE values were ≥0.35 kU/L.

Fitzpatrick A.M., Lee T., Vickery B.P., Corace E.A., Mason C., Norwood J., Caldwell C, & Grunwell J.R. (2023). Social determinants of health influence preschool and caregiver experiences during symptoms and exacerbations of wheezing. Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, 131(1).

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