Log In Sign up
The largest database of trusted experimental protocols

Multiplex immunoassay kit

Manufactured by Mesoscale
Visit Supplier
Sourced in United States

The Multiplex immunoassay kit is a lab equipment product that allows for the simultaneous detection and quantification of multiple analytes in a single sample. It utilizes a multiplexed approach to analyze various biomolecules, such as proteins, cytokines, or antibodies, in a time- and cost-effective manner.

Automatically generated - may contain errors

Lab products found in correlation

Msd discovery workbench 4, by Mesoscale (1 mentions) Il 10 elisa kit, by Thermo Fisher Scientific (1 mentions) Chemwell 2910 analyzer, by Awareness Technology (1 mentions) Oxldl elisa kit, by Cusabio (1 mentions)

Close spelling variants of this product

U‐Plex human multiplex immunoassay kits Multiplex kits

4 protocols using multiplex immunoassay kit

1

Inflammatory Cytokines and α-Synuclein Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
Brain, colon tissues, and serum levels of cytokines (TNF-α, IL-6, IL-1β, and IL-10) were measured using a multiplex immunoassay kit (K15048D) and α-synuclein levels in colon and brain tissues were quantified using an immunoassay kit (K151TGD) from Meso Scale Discovery (Rockville, MD). The assays were performed using a QuickPlex SQ120 analyzer with MSD Discovery Workbench 4.0 software (Meso Scale Discovery, Rockville, MD) according to the manufacturer’s instructions with samples run in duplicate.
Kishimoto Y., Zhu W., Hosoda W., Sen J.M, & Mattson M.P. (2019). Chronic Mild Gut Inflammation Accelerates Brain Neuropathology and Motor Dysfunction in α-Synuclein Mutant Mice. Neuromolecular medicine, 21(3), 239-249.
+ Open protocol
+ Expand
2

Cytokine Profiling in Stroke Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
Brain extracts were obtained from the peri-infarct area and the infarct core of rats subjected either to tMCAO or sham operation, respectively, and treated either with levodopa/benserazide (20 mg/kg) or vehicle (saline) for 12 days in total. Samples were diluted with lysis buffer and analyzed for the following cytokines: IL-1β, chemokine (C-X-C motif) ligand 1 (CXCL1), IL-4, IL-5, TNF-α, IFN-γ and IL-13 by a multiplex immunoassay kit according to manufacturer’s protocol (Mesoscale, Gaithersburg, MD, USA) and as described previously [22 (link)].
Kuric E, & Ruscher K. (2014). Dynamics of major histocompatibility complex class II-positive cells in the postischemic brain - influence of levodopa treatment. Journal of Neuroinflammation, 11, 145.
+ Open protocol
+ Expand
3

Multiplex Assays for Plasma Cytokines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytokine levels in plasma and media were measured using the following immunoassay kits: multiplex immunoassay kit (Meso Scale Discovery); IL-10 ELISA kit (Thermo Scientific); IL-4, IL-17, TGF-β and IFN-γ ELISA kit (eBioscience). Plasma lipids were measured using an enzymatic assay (Wako Chemicals USA, Inc.) on a ChemWell 2910 analyzer (Awareness Technology, Inc). Oxidized LDL cholesterol was measured using the OxLDL ELISA kit (CUSABIO Life Science).
Kang J.G., Sung H.J., Amar M.J., Pryor M., Remaley A.T., Allen M.D., Noguchi A.C., Springer D.A., Kwon J., Chen J., Park J.H., Wang P.Y, & Hwang P.M. (2016). Low ambient oxygen prevents atherosclerosis. Journal of molecular medicine (Berlin, Germany), 94(3), 277-286.
+ Open protocol
+ Expand
4

Multiplex Cytokine Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The levels of different cytokines—namely interferon-gamma (IFNγ), interleukin-1beta (IL-1β), IL-6 and tumor necrosis factor-alpha (TNF-α)–were evaluated using a multiplex immunoassay kit according to the manufacturer’s protocol (Mesoscale, Gaithersburg, MD, USA) in the five experimental groups tested. For this evaluation 50 μg of total protein from the protein extracts and 50 μL of serum collected from the same mice were used.
Saraiva C., Talhada D., Rai A., Ferreira R., Ferreira L., Bernardino L, & Ruscher K. (2018). MicroRNA-124-loaded nanoparticles increase survival and neuronal differentiation of neural stem cells in vitro but do not contribute to stroke outcome in vivo. PLoS ONE, 13(3), e0193609.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!