Different fractions of EPB41L4A-AS1 were constructed with the pcDNA3.1 plasmid containing the T7 promoter. The RNA pulldown assay was performed following the protocol of the Pierce Magnetic RNA-Protein Pull-Down Kit (Thermo Fisher Scientific, USA). Antibodies, including SET1A and H3K4me3, were purchased from Cell Signaling Technology.
Set1a
Manufactured by Cell Signaling Technology
SET1A is a protein that functions as a histone methyltransferase, which catalyzes the methylation of lysine 4 on histone H3. This modification is associated with active transcription.
Automatically generated - may contain errors
Lab products found in correlation
Pierce magnetic rna protein pull down kit, by Thermo Fisher Scientific (1 mentions)
H3k4me3, by Cell Signaling Technology (1 mentions)
Anti flag m2 agarose bead, by Merck Group (1 mentions)
Horseradish peroxidase conjugated goat anti mouse and anti rabbit secondary antibodies, by Thermo Fisher Scientific (1 mentions)
γh2ax, by Cell Signaling Technology (1 mentions)
Streptavidin conjugated agarose beads, by Merck Group (1 mentions)
Cd11a, by Abcam (1 mentions)
Smyd3, by Cell Signaling Technology (1 mentions)
Cd11b, by Abcam (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
2 protocols using set1a
1
Examining EPB41L4A-AS1 RNA Interactions
2
Mapping Methylation Sites in cGAS Protein
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The rabbit polyclonal anti-cGAS K491me antibody was produced by Boer Biotechnology (Chengdu, China). The mouse cGAS K491me peptide was synthesized and injected into rabbits. An affinity column with bound nonmodified peptide was used to collect and purify rabbit serum, excluding antibodies targeting nonmethylated cGAS. The antibody was then purified using an affinity column linked with bound cGAS K491me peptide. A rabbit polyclonal antibody recognizing human cGAS K506me was produced following the same protocol.
Antibodies recognizing cGAS, Tubulin, GST, His, SET1A, SET1B, SET7, MLL1, MLL3, γH2A.X and SMYD3 were obtained from Cell Signaling Technology. Antibodies recognizing RIOX2 were purchased from Atlas Antibodies. Antibodies recognizing RIOX1, ASH1L, PARP1, MLL2, SGF29, Timeless, NSD1, NSD2, H2A.X, CD11b, CD11a, CD34, Thy-1, and SMYD2 were purchased from Abcam. The antibody against Flag, anti-Flag M2 agarose beads, streptavidin-conjugated agarose beads, biotin, bovine serum albumin and poly(ADP-ribose) glycohydrolase (PARG) recombinant protein were purchased from Sigma. Horseradish peroxidase-conjugated goat anti-mouse and anti-rabbit secondary antibodies were purchased from Thermo Fisher Scientific. biotin (terminal)-PAR Polymer was purchased from Trevigen.
Antibodies recognizing cGAS, Tubulin, GST, His, SET1A, SET1B, SET7, MLL1, MLL3, γH2A.X and SMYD3 were obtained from Cell Signaling Technology. Antibodies recognizing RIOX2 were purchased from Atlas Antibodies. Antibodies recognizing RIOX1, ASH1L, PARP1, MLL2, SGF29, Timeless, NSD1, NSD2, H2A.X, CD11b, CD11a, CD34, Thy-1, and SMYD2 were purchased from Abcam. The antibody against Flag, anti-Flag M2 agarose beads, streptavidin-conjugated agarose beads, biotin, bovine serum albumin and poly(ADP-ribose) glycohydrolase (PARG) recombinant protein were purchased from Sigma. Horseradish peroxidase-conjugated goat anti-mouse and anti-rabbit secondary antibodies were purchased from Thermo Fisher Scientific. biotin (terminal)-PAR Polymer was purchased from Trevigen.
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