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Ix71 inverted tirf research microscope

Manufactured by Olympus
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The IX71 inverted TIRF research microscope is a sophisticated laboratory instrument designed for advanced cellular and molecular imaging. It features a high-performance optical system and versatile configuration options to support a wide range of research applications.

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Lab products found in correlation

C10600 10b orca r2, by Hamamatsu Photonics (3 mentions)

Close spelling variants of this product

IX71 TIRF Microscope IX71 inverted microscope Inverted research microscope IX71 microscope TIRF microscope Inverted microscope

3 protocols using ix71 inverted tirf research microscope

1

Multi-Channel Total Internal Reflection Fluorescence

Cited 1 time
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The qDF set up and the theory of qDF have been described previously in detail15 (link). Here, we expended qDF to three channels (TqDF). The set up consisted of an IX71 inverted TIRF research microscope (Olympus America) with a 100 × NA 1.45 plan-apochromatic oil immersion TIRF microscopy objective and 10 mW blue (λ=488 nm), 10 mW yellow-green (λ=561 nm), and 5 mW red (λ=641 nm) diode-pumped solid-state lasers (CVI Melles Griot) as TIRF excitation light sources. Images were captured at a rate of 0.2–1 frames per second using a QV2 (Photometrics) QuadView video coupler and a 16-bit digital charge coupled device camera (Hamamatsu C10600-10B ORCA-R2). The laser shutters and camera were controlled with the SlideBook5.5 software (Intelligent Imaging Innovations). The absorption and emission peaks of the fluorochromes used in this study were, respectively, 493 and 518 nm for DL488, 562 and 576 nm for DL550, 649 and 666 nm for CellMask DeepRed. There was no bleed-through between channels. A TIRF incidence angle of θ=70° was used for all three lasers in all TqDF experiments.
Fan Z., McArdle S., Marki A., Mikulski Z., Gutierrez E., Engelhardt B., Deutsch U., Ginsberg M., Groisman A, & Ley K. (2016). Neutrophil recruitment limited by high-affinity bent β2 integrin binding ligand in cis. Nature Communications, 7, 12658.
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2

Quantitative Dual-Fluorescence Microscopy

Cited 2 times
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The qDF setup and the theory of qDF have been described previously in detail (Sundd et al., 2010 (link), 2011 (link); Wen et al., 2020 (link)). The setup was based on an IX71 inverted TIRF research microscope (Olympus) equipped with a 100x NA 1.45 plan-apochromatic oil immersion TIRFM objective and 30 mW 488 nm, 20 mW 561 nm and 5 mW 641 nm diode-pumped-solid-state lasers (CVI Melles Griot) as TIRF excitation light sources. A TIRF incidence angle of 70°was used for all three lasers in all qDF experiments. Images were captured at an interval of every 1–2 s using a 16-bit digital CCD camera (Hamamatsu C10600–10B ORCA-R2). The laser shutters and camera were controlled with the SlideBook5.5 software (Intelligent Imaging Innovations Inc.). Fluorescence channels were separated using a beam splitter (QV2 QuadView; Photometrics) equipped with two dichroic mirrors (560 nm and 660 nm) and three emission filters (525/50 nm, 600/32 nm, and 700/75 nm).
Wen L., Marki A., Wang Z., Orecchioni M., Makings J., Billitti M., Wang E., Suthahar S.S., Kim K., Kiosses W.B., Mikulski Z, & Ley K. (2022). A humanized integrin knockin mouse reveals localized intra- and extravascular neutrophil integrin activation in vivo. Cell reports, 39(9), 110876.
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3

Quantitative Dual-Fluorescence Microscopy

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The qDF setup and the theory of qDF have been described previously in detail (Sundd et al., 2010 (link), 2011 (link); Wen et al., 2020 (link)). The setup was based on an IX71 inverted TIRF research microscope (Olympus) equipped with a 100x NA 1.45 plan-apochromatic oil immersion TIRFM objective and 30 mW 488 nm, 20 mW 561 nm and 5 mW 641 nm diode-pumped-solid-state lasers (CVI Melles Griot) as TIRF excitation light sources. A TIRF incidence angle of 70°was used for all three lasers in all qDF experiments. Images were captured at an interval of every 1–2 s using a 16-bit digital CCD camera (Hamamatsu C10600–10B ORCA-R2). The laser shutters and camera were controlled with the SlideBook5.5 software (Intelligent Imaging Innovations Inc.). Fluorescence channels were separated using a beam splitter (QV2 QuadView; Photometrics) equipped with two dichroic mirrors (560 nm and 660 nm) and three emission filters (525/50 nm, 600/32 nm, and 700/75 nm).
Wen L., Marki A., Wang Z., Orecchioni M., Makings J., Billitti M., Wang E., Suthahar S.S., Kim K., Kiosses W.B., Mikulski Z, & Ley K. (2022). A humanized integrin knockin mouse reveals localized intra- and extravascular neutrophil integrin activation in vivo. Cell reports, 39(9), 110876.
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