As1842856

Yellow catfish were obtained from Hubei Bairong Fisheries Farm (Huanggang, Hubei Province, China). HepG2 cell lines were obtained from the Cell Resource Center in the Fishery College of Huazhong Agricultural University. Dulbecco’s Modified Eagles Medium (DMEM), 0.25% trypsin-EDTA and fetal bovine serum (FBS) were obtained from Gibco/Invitrogen, USA. AS1842856, dimethyl sulphoxide (DMSO), penicillin, streptomycin, trypan blue and other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). We ensured that the experiments were performed in accordance with the experimental protocols of Huazhong Agricultural University (HZAU) and approved by the ethics committee of HZAU (identification code: Fish-2016-0419, Date: 19 April 2016)

SW480 and SW620 cells were cultured in L15 medium (Invitrogen, NY, USA) at 37°C without CO₂. Colon cancer cells (HCT116 and HT29), prostate cancer cells (PC3), hepatoma cells (Huh7 and Hep3B), lung cancer cells (A549), gastric cancer cells (AGS), and breast cancer cells (MCF-7) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Invitrogen) at 37°C with 5% CO₂. All of these cells were supplemented with 10% fetal bovine serum (FBS; Biological Industries, Kibbutz Beit Haemek, Israel) and penicillin/streptomycin (Sigma, MO, USA). Amiodarone, chloroquine (CQ), and FoxO1 inhibitor AS1842856 were purchased from Sigma.

16,16‐dimethyl prostaglandin E₂ (dm‐PGE₂) and PGE₂ were purchased from Cayman Chemical. Highly selective agonists for EP1 (ONO‐DI‐004), EP2 (ONO‐AE1‐259–01), EP3 (ONO‐AE‐248) or EP4 (ONO‐AE1‐329) were gifts from Ono Pharmaceutical Co., Japan. Selective antagonists against EP2 (PF‐04418948) and EP4 (L‐161,982) were purchased from Cayman. Recombinant human TGF‐β1 and mouse or human IL‐2 were purchased from R&D system or Biolegend. Indomethacin, dibutyryl‐cAMP (db‐cAMP), 3‐isobutyl‐1‐methylxanthine (IBMX), H‐89, LY‐294002, AS1842856 and STAT5 inhibitor were purchased from Sigma or Calbiochem.

All cell lines used in this study were provided by the American Type Culture Collection (Manassas, VA, USA). U87MG cells were propagated in MEM (Minimal Essential Medium, Thermo Fisher, Waltham, MA). DBTRG cells were propagated in RPMI (Roswell Park Memorial Institute 1640 Medium, Thermo Fisher, Waltham, MA). LN-18, U118 MG, and LN-229 cells were propagated in DMEM (Dulbecco’s Modified Eagle Medium, Thermo Fisher, Waltham, MA, USA). Cell lines were grown in standard propagating conditions supplemented with 10% fetal bovine serum (FBS, Thermo Fisher, Waltham, MA), antibiotic (Anti-Anti, Gibco cat: 15240096, Thermo Fisher, Waltham, MA), and 5% CO₂ at 37 °C. The dual PI3K-mTOR pathway inhibitor NVP-BEZ235 and FOXO1 inhibitor AS1842856 were acquired from Sigma-Aldrich (Saint Louis, MO, USA) and Calbiochem (Danvers, MA, USA), respectively. NVP-BEZ235 and AS1842856 were utilized in final concentrations at 50 nM (or 1 μM) and 200 nM, respectively. Control samples were treated with DMSO.

Recombinant human CCL2 for cell treatments was obtained from R&D (MN, USA; #279-MC-050). AS1842856 (#344355) and phorbol 12-myristate 13-acetate (PMA, #P8139) were obtained from Sigma–Aldrich (MO, USA). WY-14643 (#S8029), GW4671 (#S2798), SB203580 (#S1076), SP600125 (#S1460), and SCH772984 (S7101) were obtained from Selleck (Shanghai, China). The Dual-Luciferase Reporter Assay System (E1910) was obtained from Promega (WI, USA). The SimpleChIP (chromatin immunoprecipitation) Plus Sonication Chromatin IP Kit (#56383) and Alexa Fluor 488–conjugated CD68 antibody (#24850) were purchased from Cell Signaling Technology (MA, USA). The Alexa Fluor 647–conjugated CCR2 antibody (#ab225432) was obtained from Abcam (Cambridge, UK). The Lipofectamine 2000 (#11668019), negative control (miR-NC), miR-580-5p mimic (#4464066), and inhibitor (AM17000) were obtained from Thermo Fisher Scientific (Runcorn, Cheshire, UK). siRNA- Zinc Finger Protein 562 (ZNF562), siRNA-circ-102231, and the negative control were purchased from GenePharma Biotechnology (Shanghai, China). The siRNA target site of hsa_circ_0110102 was 5′-ACAGTGGAGAAAGGTAAATGCAA-3′, and that of ZNF562 was 5′-GTCATTGATAACATCTTATCAGG-3′. The construction of hsa_circ_0110102 overexpression in the Ubi-MCS-Luc-IRES-Puromycin vector was performed by Biosyntech Co., Ltd (Suzhou, China).

Cell lines derived from GCB-DLBCLs (i.e., OCI-Ly1, OCI-Ly2, SU-DHL4, SU-DHL5, and SU-DHL8) and ABC-DLBCLs (i.e., OCI-Ly10 and SU-DHL9) were kindly provided by Prof. Megan S. Lim (University of Michigan, Ann Arbor, MI, USA). All of the cell lines were cultured and subtypes in terms of the cell-of-origin according to the instructions of the American Type Culture Collection or the German Collection of Microorganisms and Cell Cultures. Actinomycin D, LY294002, AS1842856, vinblastine and doxorubicin were all purchased from Sigma (St. Louis, MO, USA).