Bio plex pro rat cytokine 24 plex assay

Manufactured by Bio-Rad

Sourced in Canada, United States, Sweden

The Bio-Plex Pro™ Rat Cytokine 24-plex Assay is a multiplex immunoassay designed to measure the concentration of 24 different cytokines in rat biological samples. It enables the simultaneous quantification of multiple analytes from a single sample.

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Lab products found in correlation

Bio plex 100 system, by Bio-Rad (1 mentions) Sample diluent, by Bio-Rad (1 mentions) Bio plex 3d suspension array system, by Bio-Rad (1 mentions) Bio plex 200 system, by Bio-Rad (1 mentions) Bio plex 200 system with htf, by Bio-Rad (1 mentions)

6 protocols using bio plex pro rat cytokine 24 plex assay

Cytokine and sFlt-1 Profiling in LPS-Induced Rat Model

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Rat plasma samples from animals treated with LPS (n = 7), saline (n = 3), or LPS+GTN (n = 3) were analysed using a Bio-Plex Pro Rat Cytokine 24-plex Assay (Bio-Rad, Mississauga, Canada; cat. #171-K1001M) according to the manufacturer’s instructions. The levels of all 24 factors were evaluated in this study, but only those samples with cytokine concentrations above the detectable range were included for analysis. Therefore, we were unable to perform statistical analysis for the following factors: IL-1 Ra, IL-1 β, IL-4, IL-7, IL-17A, IL-12p70 and GROα.
sFlt-1 detection was performed on plasma samples obtained from LPS (N = 10 dams), saline (N = 8), and LPS+GTN (N = 8) treated dams on GD 17.5 using the Rat VEGFR1 ELISA (RayBiotech, Norcross, USA, cat. ELR-VEGFR1-1) according to the manufacturer’s instructions. Grubb’s outlier test was used to identify outliers in each group; one data point was subsequently removed from the LPS+GTN group.

Robb K.P., Cotechini T., Allaire C., Sperou A, & Graham C.H. (2017). Inflammation-induced fetal growth restriction in rats is associated with increased placental HIF-1α accumulation. PLoS ONE, 12(4), e0175805.

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Multiplex Cytokine Profiling of Rat Serum

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Serum cytokine levels were determined using the Bio-Plex Pro^™ Rat Cytokine 24-Plex Assay (Catalog No. 171K1001M; Bio-Rad, Hercules, CA, USA) following the manufacturer’s protocol. In brief, after thawing, serum was centrifuged again at 10 000 × g for 10 min at 4 °C to remove precipitates and platelets. Samples were diluted 1:4 in Bio-Rad sample diluent. Plate was read at High RP1 (PMT) setting using the Bio-Plex 100 system (Bio-Rad, Hercules, CA, USA). A complete list of cytokines tested is found in Table 2.

Russell A.L., Grimes J.M., Cruthirds D.F., Westerfield J., Wooten L., Keil M., Weiser M.J., Landauer M.R., Handa R.J., Wu T.J, & Larco D.O. (2017). Dietary Isoflavone-Dependent and Estradiol Replacement Effects on Body Weight in the Ovariectomized (OVX) Rat. Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme, 49(6), 457-465.

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Multiplex Biomarker Profiling in Rat

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Cytokine concentrations in rat serum and adipose tissue were determined using the Bio‐Plex Pro Rat Cytokine 24‐plex Assay multiplex immunoassays (Bio‐Rad, Hercules, CA) and the final data were obtained and analyzed via the Bio‐plex 3D Suspension array system (Bio‐Rad). For the determination of intracellular kinases activation levels we used a combination of Bio‐plex nonmagnetic signal transduction assays (Bio‐Rad). Loading for the phosphoprotein panels as well as the adipose tissue cytokine panels was normalized via determination of total protein concentration (BCA Protein Assay kit, Pierce, Rockford, IL) and measurement of β‐actin levels after western immunoblot analysis.

Karagiannides I., Golovatscka V., Bakirtzi K., Sideri A., Salas M., Stavrakis D., Polytarchou C., Iliopoulos D., Pothoulakis C, & Bradesi S. (2014). Chronic unpredictable stress regulates visceral adipocyte‐mediated glucose metabolism and inflammatory circuits in male rats. Physiological Reports, 2(5), e00284.

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Multiplex Cytokine Profiling in Rat Plasma

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The endpoint plasma concentrations of the cytokines [sensitivities in square brackets] tumor necrosis factor-α (TNF-α) [3 pg/ml], interleukin(IL)-1α [1 pg/ml], IL-1β [2 pg/ml], IL-2 [3 pg/ml], IL-4 [1 pg/ml], IL-5 [6 pg/ml], IL-7 [0.4 pg/ml], IL-10 [5 pg/ml], IL-12p70 [0.7 pg/ml], IL-13 [0.9 pg/ml], IL-17A [0.1 pg/ml], IL-18 [4 pg/ml], macrophage colony-stimulating factor (M-CSF) [0.4 pg/ml], monocyte chemotactic protein 1 (MCP-1) [4 pg/ml], macrophage inflammatory protein (MIP)-3α [0.7 pg/ml], regulated on activation normal T cell expressed and secreted (RANTES) [3 pg/ml], erythropoietin (EPO) [8 pg/ml], granulocyte–macrophage colony-stimulating factor (GM-CSF) [0.6 pg/ml], growth-related oncogene/keratinocyte-derived chemokine (GRO/KC) [0.6 pg/ml] and vascular endothelial growth factor (VEGF) [0.3 pg/ml] were analyzed using multiplex Bio-Plex Pro™ Rat Cytokine 24-plex Assay and Bio-Plex 200 system (Bio-Rad, Sweden). The concentrations of the cytokines were expressed as mean (range) pg/ml.

Eriksson K., Lönnblom E., Tour G., Kats A., Mydel P., Georgsson P., Hultgren C., Kharlamova N., Norin U., Jönsson J., Lundmark A., Hellvard A., Lundberg K., Jansson L., Holmdahl R, & Yucel-Lindberg T. (2016). Effects by periodontitis on pristane-induced arthritis in rats. Journal of Translational Medicine, 14, 311.

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Multiplex Cytokine Analysis in Rats

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Multiplex analysis based on the xMAP Luminex technology was performed with the use of a kit for Bio-Plex Pro™ Rat Cytokine 24-plex Assay # 171K1001M (Bio-Rad), according to the manufacturer’s instructions. Experiments were performed in triplicate. The kit enables a simultaneous multiplex analysis of 24 cytokines, chemokines, and interleukins rat in a 50-µl sample.

Mukhamedshina Y.O., Akhmetzyanova E.R., Martynova E.V., Khaiboullina S.F., Galieva L.R, & Rizvanov A.A. (2017). Systemic and Local Cytokine Profile following Spinal Cord Injury in Rats: A Multiplex Analysis. Frontiers in Neurology, 8, 581.

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Multiplex Cytokine Profiling in Stroke Model

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Separate cohorts of rats of both sexes exposed to tMCAO and tMCAO + WBV/No-WBV were sacrificed at 24 h and 1 month after tMCAO respectively, for brain tissue and blood/serum collection (details of euthanasia methods are provided in the Supplementary Data). Serum samples were used for the Bio-Plex assay. The Bio-Plex immunoassays are volume-sparing, requiring a total of only 50 μl of serum to run each sample in duplicate. Serial dilutions of standards representing each analyte and an in-house control plasma sample were run in duplicate on each 96-well assay plate. Fluorescence intensities of each analyte-specific immunoassay bead were analyzed on the Bio-plex 200 System with HTF (Bio-Rad). Raw data were captured using Bio-Plex Manager Software 6.1. A concentration of individual immune factors in each sample was interpolated from standard curves using a five-parameter, weighted logistic regression curve equation in Bioplex Manager Software 6.1. We used the Bio-Plex Pro™ Rat Cytokine 24-plex Assay (cat #171K1001M from Bio-Rad) and additional data are presented in Supplementary Tables 2–5. The 24 analytes chosen for Bio-Plex assay are based on functional immune markers categories, which are associated with inflammatory and immune-mediated injury in cerebrovascular and neuropsychiatric disorders, including dementia.

Kerr N., Sanchez J., Moreno W.J., Furones-Alonso O.E., Dietrich W.D., Bramlett H.M, & Raval A.P. (2022). Post-stroke low-frequency whole-body vibration improves cognition in middle-aged rats of both sexes. Frontiers in Aging Neuroscience, 14, 942717.

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