The TNBC cell lines BT549, MB468, MB231, HCC1937, and 4T1 were purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). HEK-293T cell line was purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). SUM149 and SUM159 cells were obtained from the State Key Laboratory of Biotherapy of Sichuan University (Chengdu, China). MB468, MB231, and SUM149 cells were cultured in a humidified of 5% CO2 at 37°C in DMEM (Gibco) medium supplementing with 10% fetal bovine serum (FBS) (NATOCOR) and 1% penicillin-streptomycin (100 μg/mL, Hyclone). BT549, HCC1937, SUM159, and 4T1 cells were cultured in a humidified of 5% CO2 at 37°C in RPMI (Gibco) medium supplementing with 10% fetal bovine serum and 1% penicillin-streptomycin. All cell lines were confirmed mycoplasma negative by end-point PCR.
Mb231
Manufactured by Thermo Fisher Scientific
Sourced in United States
The MB231 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed for general laboratory use. The core function of the MB231 is to provide a stable and controlled environment for various laboratory procedures. Detailed specifications and intended use are not available.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Bt 549, by Thermo Fisher Scientific (1 mentions)
Bt 549, by National Collection of Authenticated Cell Cultures (1 mentions)
Hek 293 t cell line, by National Collection of Authenticated Cell Cultures (1 mentions)
Sum 159, by Thermo Fisher Scientific (1 mentions)
Hcc1937, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Natocor (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Mcf 10a, by Thermo Fisher Scientific (1 mentions)
Cm 0525, by Procell (1 mentions)
Au 565, by Thermo Fisher Scientific (1 mentions)
Mcf 7, by Thermo Fisher Scientific (1 mentions)
Mcf 10a, by Procell (1 mentions)
2 protocols using mb231
1
Cultivation Conditions for Triple-Negative Breast Cancer Cell Lines
2
Knockdown of IDH2 in Breast Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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MCF-10A, MB-231, MCF-7 and AU-565 were purchased from ATCC (American Type Culture Collection) with STR (Short Tandem Repeat) certifications and were cultured in DMEM (Gibco, USA) with 10% FBS (Cellbox, China) and 1% P/S (Procell, China) at 37 °C and 5% CO2 conditions, except for MCF-10A, which was cultured in special medium (CM-0525, Procell, China).
MB-231 cells were cultured in 6-well plates at 2 × 105 cells per well, and 12 h later, virus infection (pSLenti-U6-shRNA(IDH2)-CMV-EGFP-Puro-WPRE, 50 nmol/L) and 10 μL of 1 mg/mL polybrene were added to each well, making a final working concentration of 5 μg/mL. The medium was changed after 12 h of infection. After 72 h, a final concentration of 1 μg/mL puromycin was added. Fresh medium was then changed every 2–3 days with a final concentration of 1 μg/mL puromycin. After 4 weeks of drug screening, fluorescence photographic screening was performed. Three different siRNA-IDH2 (NM_002168.4) were constructed and screened the one with the best knockdown effect for subsequent experiments. The target sequence of the siRNA was CCAAGAACACCATACTGAAAG.
MB-231 cells were cultured in 6-well plates at 2 × 105 cells per well, and 12 h later, virus infection (pSLenti-U6-shRNA(IDH2)-CMV-EGFP-Puro-WPRE, 50 nmol/L) and 10 μL of 1 mg/mL polybrene were added to each well, making a final working concentration of 5 μg/mL. The medium was changed after 12 h of infection. After 72 h, a final concentration of 1 μg/mL puromycin was added. Fresh medium was then changed every 2–3 days with a final concentration of 1 μg/mL puromycin. After 4 weeks of drug screening, fluorescence photographic screening was performed. Three different siRNA-IDH2 (NM_002168.4) were constructed and screened the one with the best knockdown effect for subsequent experiments. The target sequence of the siRNA was CCAAGAACACCATACTGAAAG.
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