Ultrospec 2100 pro spectrophotometer
The Ultrospec 2100 Pro is a high-performance spectrophotometer designed for a wide range of applications in laboratories. It offers a wavelength range of 190 to 900 nm and can measure absorbance, transmittance, and concentration. The Ultrospec 2100 Pro provides accurate and reproducible results with its advanced optics and digital signal processing technology.
Lab products found in correlation
30 protocols using ultrospec 2100 pro spectrophotometer
MTT Cytotoxicity Assay Protocol
Preparation of Staphylococcus aureus Glycerol Stocks
S. aureus USA300 WT) and an isogenic mutant (
S. aureus USA300
Δlip1/Δlip2) of the CC8 epidemic clone
S. aureus USA300 LAC generated in a previous study were stored at -80°C. When required, stocks were sub-cultured onto tryptone soy agar (TSA, Oxoid CM131B) or cultured into tryptone soy broth (TSB, Oxoid CM129B) overnight at 37°C with agitation (200 rpm). The culture was diluted 1 in 100 in TSB and incubated, until exponential phase (OD
600=0.6–0.8), as measured using an Amersham Biosciences Ultrospec 2100 pro spectrophotometer. For infection protocols, bacteria were washed in cell culture media and suspended at the required OD
600.
Phascolarctobacterium faecium Growth Kinetics
Phascolarctobacterium faecium JCM 30894 from a 6‐day plate culture were suspended in phosphate‐buffered saline (PBS). A 1% (v/v) suspension (MacFarland standard 3 turbidity) was inoculated into Gifu Anaerobic Medium Broth (GAM Broth, Nissui Pharmaceutical Co., Tokyo, Japan) that was not supplemented or supplemented with 1% (w/v) succinate (adjusted to pH 7.0). The broth was cultured experiments that were performed in anaerobic serum bottles sealed with butyl‐rubber stoppers at 37°C in an atmosphere of CO2 and N2 (1:9, v/v). Cultures were sampled at 0, 18, 20, 22, 24, 42, 44, 46, and 48 hr for analysis of metabolites and measurements of optical density at 660 nm (OD660). OD660 was measured using an Ultrospec 2100 pro spectrophotometer (Amersham Biosciences, Piscataway, NJ, USA). The pH of the medium was measured using a Twin pH compact pH meter (HORIBA, Kyoto, Japan).
Cultivation and Metabolic Analysis
Quantitative PCR of Salmonella Typhimurium
All qPCRs were performed on an Applied Biosystems 7300 real-time PCR system. A 25 µl reaction contained 12.5 µl of FastStart SYBR Green Master Mix with Rox (Roche, 04913914001), 8 µl DNase/RNase-free water, 0.75 µl of forward and reverse (10 µM) primers (
Artificial Sweetener Effects on Bacterial Growth
RNA Isolation and cDNA Synthesis
Dynein Labeling Efficiency Characterization
Analytical Techniques for Fermentation Metabolites
Concentrations of glucose, xylose, xylitol, glycerol, succinate, acetate and ethanol were analysed by HPLC (Waters, USA). Aminex HPX-87H ion exchange column (Bio-Rad, USA) was used at 45°C with a mobile phase of 5 mM H2SO4 at a flow rate of 0.6 mL/min. All compounds were detected with a RID-10A refractive index detector (Shimadzu, Japan).
Nitrate Reduction Assay Optimization
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