Maxis 3g orthogonal acceleration quadrupole time of flight mass spectrometer qtof ms

Chemical analysis was performed using a maXis 3G orthogonal acceleration quadrupole time-of-flight mass spectrometer (QTOF-MS) (Bruker Daltonics) connected to an Ultimate 3000 UHPLC system (Dionex). Separation was achieved using a Kinetex 1.7-μm C₁₈, 100 mm by 2.1 mm column (Phenomenex). The column temperature was maintained at 40°C throughout the analysis, and a linear gradient using LC-MS-grade water and acetonitrile both buffered with 20 mM LC-MS-grade formic acid, starting from 10% (vol/vol) acetonitrile and increased to 100% in 10 min, maintaining this rate for 3 min before returning to the starting conditions in 0.1 min and staying there for 2.4 min before the following run. A flow rate of 0.4 ml · min⁻¹ was used. Mass spectrometric detection was performed in ESI⁺ with a data acquisition frequency of 10 scans per second in the m/z range 75 to 1,250. Mass calibration was done using Bruker Daltonics high-precision calibration algorithm by automatically infusing the internal standard sodium formate before each run. UV-visible (UV-Vis) absorption spectra were collected at wavelengths from 190 to 800 nm. Data processing and analysis were performed using DataAnalysis 4.0.

Chemical analysis was performed using a maXis 3G orthogonal acceleration quadrupole time-of-flight mass spectrometer (QTOF-MS) (Bruker Daltonics) connected to an Ultimate 3000 UHPLC system (Dionex). Separation was achieved using a Kinetex 1.7-μm C₁₈, 100 mm by 2.1 mm column (Phenomenex). The column temperature was maintained at 40°C throughout the analysis, and a linear gradient using LC-MS-grade water and acetonitrile both buffered with 20 mM LC-MS-grade formic acid, starting from 10% (vol/vol) acetonitrile and increased to 100% in 10 min, maintaining this rate for 3 min before returning to the starting conditions in 0.1 min and staying there for 2.4 min before the following run. A flow rate of 0.4 ml · min⁻¹ was used. Mass spectrometric detection was performed in ESI⁺ with a data acquisition frequency of 10 scans per second in the m/z range 75 to 1,250. Mass calibration was done using Bruker Daltonics high-precision calibration algorithm by automatically infusing the internal standard sodium formate before each run. UV-visible (UV-Vis) absorption spectra were collected at wavelengths from 190 to 800 nm. Data processing and analysis were performed using DataAnalysis 4.0.