Cells after 24h-treatment by lidocaine (Lid), BMSC-exo, or oePDCD4 plasmids were stained by Annexin V Apoptosis Kit (Beyotime) and a Beckman CytoFLEX Flow Cytometer was used to analyze apoptosis.
Annexin 5 apoptosis kit
Manufactured by Beyotime
Sourced in China
The Annexin V Apoptosis Kit is a laboratory product designed to detect and quantify apoptosis, a specific type of programmed cell death. The kit provides the necessary reagents to label and analyze cells undergoing apoptosis using flow cytometry or fluorescence microscopy.
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8 protocols using annexin 5 apoptosis kit
1
Apoptosis Analysis of Treated Cells
2
Annexin-V Apoptosis Assay in HeLa Cells
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HeLa cells were treated with different concentrations of compound 3 or 4 for 24 h. The HeLa cells were harvested and washed. Staining of Annexin-V was done by using the Annexin V Apoptosis Kit (Beyotime, Nantong, China) according to the manufacturer’s instructions. After incubation at room temperature for 20 min in the dark, cells were stained by PI and immediately detected by flow cytometry (BD Biosciences, San Jose, CA, USA).
3
Annexin V Apoptosis Assay Protocol
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Cell apoptosis was calculated by Annexin V apoptosis kit (Beyotime, China), and the operating procedure was according to the kit instructions. Briefly, 5×105 cells/mL were centrifuged and resuspended in with Annexin V-FITC and PI solution in darkness for 15 min. Then, Binding Buffer was mixed into the resuspension and detected with instrument. Cell apoptosis level was detected within 1 h.
4
Angiotensin II-Induced Cardiomyocyte Apoptosis
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Primary rat cardiomyocyte cells after 24 h treatment with Ang II, Ang II + 0.2 mg/ml HQQR, Ang II + 0.5 mg/ml HQQR, Ang II + 10 µmol/l valsartan were stained using Annexin V Apoptosis kit (C1062; Beyotime Institute of Biotechnology) according to the manufacturer's instructions and a Beckman CytoFLEX Flow Cytometer (Beckman Coulter, Inc.) with FlowJo software (v10.0.7r2; Tree Star, Inc.) was used to analyze apoptosis.
5
Annexin V-based Apoptosis Quantification
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Cell apoptosis was calculated by Annexin V apoptosis kit (Beyotime, China), and the operating procedure was according to the kit instructions. A total of 5 × 105 cells/ml were centrifuged and resuspended in 200 μl Binding Buffer with 10 ml Annexin V-FITC and 10 μl PI solution at room temperature in darkness for 15 min after which 300 μl Binding Buffer was mixed into the resuspension. Cell apoptosis level was detected within 1 h.
6
Annexin V-FITC Apoptosis Assay
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Annexin V Apoptosis Kit (Beyotime) was exploited for apoptosis analysis of transfected cells. Then, 200 μl binding buffer was employed to resuspend cells, followed by staining of 2 × 105 cells with 10 μl Annexin V‐fluorescein isothiocyanate (FITC) and propidium iodide (PI). Twenty minutes later, apoptotic cells were observed under the flow cytometer (BD Biosciences). Cell apoptosis rate was indicated by apoptotic cells/total cells × 100%.
7
Annexin V Apoptosis Assay Protocol
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Cell apoptosis was calculated by Annexin V apoptosis kit (Beyotime, China), and the operating procedure was according to the kit instructions. Briefly, 5×105 cells/mL were centrifuged and resuspended in with Annexin V-FITC and PI solution in darkness for 15 min. Then, Binding Buffer was mixed into the resuspension and detected with instrument. Cell apoptosis level was detected within 1 h.
8
Resveratrol and IRA-3 Effects on Apoptosis
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Cells were seeded in six‐cell plate and incubated overnight at 37 °C and then incubated with H2O2 (100 μm ) for 6 h followed by 50 μm resveratrol or IRA‐3 treatment for 24 h. After that, the cells were stained with annexin V and PI using an annexin V apoptosis kit (C1062M; Beyotime Biotechnology) in accordance with the manufacturer’s instructions. The stained cells were then analyzed by flow cytometer (FC500; Beckman Colter, Indianapolis, IN, USA) to distinguish among viable (annexin V−/PI−), early apoptotic (annexin V+/PI−), late apoptotic (annexin V+/PI+) cells and necrotic cells (annexin V−/PI+).
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