Api5500 triple quadrupole linear ion trap tandem mass spectrometer

The chromatographic analysis of SYQ was performed on a SIL-20A XR system (Shimadzu, Kyoto, Japan). The separation was conducted by a XBridge^®C18 column (4.6 mm × 100 mm, 3.5 μm) at 30 °C and the injection volume was 2 µL. The mobile phase contained 0.4% formic acid water solution (A) and methanol solution (B) at 0.8 mL/min flow rate with the following gradient elutions: 0–4 min, 7–9% B; 4–6 min, 9–21% B; 6–10 min, 21–35% B; 10–12 min, 35–38% B; 12–16 min, 38–46% B; 16–20 min, 46–64% B; 20–21 min, 64–7% B.
An API5500 triple quadrupole linear ion trap tandem mass spectrometer (AB SCIEX, Framingham, MA, USA) equipped with an electrospray ionization (ESI) source was used for detection. The operating parameters were as follows: ion source temperature, 550 °C; nebulizer gas (GS1) flow, 55 L/min; auxiliary gas (GS2) flow, 55 L/min; curtain gas (CUR) flow, 40 L/min; spray voltage (IS), 4500 V in the positive mode and −4500 V in the negative mode. Detection of analytes was performed in multiple-reaction mode (MRM).

The samples of Spatholobi Caulis were analyzed by SIL–20A UFLC XR system (Shimadzu, Kyoto, Japan). The column used for chromatographic analysis was XBridge^®C₁₈ column (4.6 mm × 100 mm, 3.5 μm) at 30 °C with gradient elution of 0.3% formic acid aqueous solution (A)–methanol (B) and the flow rate was 0.8 mL/min. The injection volume was 2 μL and the elution gradient was optimized as follows: 0–5 min, 15–30% B; 5–10 min, 30–40% B; 10–13 min, 40–45% B; 13–15 min, 45% B; 15–18 min, 45–55% B; 18–23 min, 55–65% B; 23–28 min, 65–100% B; 28–30 min, 100%B.
The API5500 triple quadrupole linear ion trap tandem mass spectrometer (AB SCIEX, Framingham, MA, USA) was used to detect samples by the multiple reaction monitoring mode (MRM) under both positive and negative electrospray ionization mode (ESI). The mass spectrometry parameters were set as below: curtain gas (CUR) flow rate: 40 L/min; atomized gas (GS1) flow rate: 55 L/min; auxiliary gas (GS2) flow rate: 55 L/min; ionization temperature (TEM): 550 °C; spray voltage (IS): 4500 V in positive ion mode and −4500 V in negative ion mode.

The SIL–20A UFLC XR system (Shimadzu Co., Kyoto, Japan) was used for analysis. LC separation was performed on an XBridge^®C18 column (4.6 mm × 100 mm,3.5 µm) at 30 °C under the condition of gradient elution, with the mobile phase consisting of 0.1% formic acid (A)-MeOH(B). The gradient elution was as follows: 0–5 min, 2–27% A; 5–8 min, 27–31% A; 8–14 min, 31–32% A; 14–17 min, 32–34% A; 17–22 min, 34–40% A; 22–26 min, 40–73% A; 26–29 min, 73–2% A. The injection volume was 2 μL, and the flow rate was 0.5 mL/min.
An API5500 triple quadrupole linear ion trap tandem mass spectrometer (AB SCIEX, Framingham, MA, USA) equipped with an electrospray ionization (ESI) source was used for detection. The operating parameters were as follows: ion source temperature, 550 °C; nebulizer gas (GS1) flow, 55 L/min; auxiliary gas (GS2) flow, 55 L/min; curtain gas (CUR) flow, 40 L/min; spray voltage (IS), 4500 V in the positive mode and −4500 V in the negative mode. Detection of analytes was performed in multiple-reaction mode (MRM).