Estradiol

Manufactured by R&D Systems

Sourced in United States

Estradiol is a hormone that plays a crucial role in regulating various physiological processes, particularly in the female reproductive system. It is a key component of the estrogen family and is produced primarily by the ovaries in women. Estradiol is responsible for the development and maintenance of female sexual characteristics and is essential for the proper function of the reproductive organs and the regulation of the menstrual cycle.

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Lab products found in correlation

Osteocalcin, by R&D Systems (1 mentions) Elisa kits to detect, by R&D Systems (1 mentions) Igfbp3, by R&D Systems (1 mentions) Igf 1, by R&D Systems (1 mentions) El808 absorbance microplate reader, by Agilent Technologies (1 mentions) Amphotericin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Mtt cell proliferation assay, by American Type Culture Collection (1 mentions) Caco 2 cell, by American Type Culture Collection (1 mentions) Deadend fluorometric tunel assay kit, by Promega (1 mentions) Afuresertib, by Cayman Chemical (1 mentions) Gw3965, by R&D Systems (1 mentions) Dnase, by Thermo Fisher Scientific (1 mentions) Sw620 cells, by American Type Culture Collection (1 mentions) Immun blot pvdf membrane for protein blotting, by Bio-Rad (1 mentions) Hard set mounting medium with dapi, by Vector Laboratories (1 mentions) Leibovitz s l 15 media, by Thermo Fisher Scientific (1 mentions) Cytotox 96 non radioactive cytotoxicity assay kit, by Promega (1 mentions) Dmem media, by Thermo Fisher Scientific (1 mentions)

3 protocols using estradiol

Quantification of Hormones and Enzymes

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ELISA kits to detect alkaline phosphatase (ALP) activity and the concentrations of estradiol, osteocalcin, IGF-1, IGFBP-3, luteinizing hormone (LH), and follicle stimulating hormone (FSH) were purchased from R&D Systems (Minneapolis, USA).

Trinh T.A., Park S.C., Oh J., Kim C.E., Kang K.S., Yoo H.S, & Lee H.L. (2017). Preventive Effect and Safety of a Follicle Stimulating Hormone Inhibitory Formulation Containing a Mixture of Coicis Semen and Artemisia capillaris for Precocious Puberty: A Preliminary Experimental Study Using Female Rats. Evidence-based Complementary and Alternative Medicine : eCAM, 2017, 2906014.

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Serum Hormone Concentration Analysis

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Estradiol (R&D Systems, Minneapolis, USA) and T3 (Abbexa Ltd., Cambridge UK) serum concentrations were measured using enzyme-linked immunosorbent assay (ELISA), following the manufacturer’s instructions. Estradiol assay sensitivity reported by the manufacturer is 12.1 pg/mL, range 12.3–3,000 pg/mL, Minimum Detectable Dose 4.84 pg/ml, with an intra-assay precision of 5.97% coefficient of variation (CV) and an inter-assay precision of 7.13%. T3 assay sensitivity reported by the manufacturer is < 35 pg/mL, range 78 pg/mL—5000 pg/mL, with an intra-assay precision <7.2% CV and an inter-assay precision at <10.1% CV. Serum samples were run in duplicate and in our hands CVs of 5.7% for E2 and 4.1% for T3 were attained. The mean of each sample was taken as the hormone concentration. Samples were then analyzed using an EL808™ Absorbance Microplate Reader (BioTek, Vermont, US) and software Gen5 (version 1.11; BioTek, Vermont, US). There was insufficient serum volume for 13 participants when undertaking analysis of T3 resulting in a sample size of 48 for the T3 data.

Onambélé-Pearson G.L., Tomlinson D.J., Morse C.I, & Degens H. (2021). A prolonged hiatus in postmenopausal HRT, does not nullify the therapy’s positive impact on ageing related sarcopenia. PLoS ONE, 16(5), e0250813.

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Caco2 and SW620 Cell Proliferation Assay

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Caco2 cells (cat # HTB-37™), MTT Cell Proliferation Assay (cat # 30–1010K), and SW620 cells (cat # CCL-227™) (ATCC, Manassas, VA). DMEM media, Leibovitz’s L-15 media, 100U/ml penicillin, 100ug/ml streptomycin, and 0.25ug/ml amphotericin (Life Technologies, Carlsbad, CA). FBS (Atlanta Biologicals, Flowery Branch, GA). GW3965 (cat # 2474), 27-hydroxycholesterol (cat # 3907), and estradiol (cat # 2824) (R&D systems from Minneapolis, MN). 6α-epoxycholesterol-3-sulfate (cat # C4136–000) (Steraloids, Newport, RI). Afuresertib (cat # 17988) (Cayman Chemicals from Ann Arbor, MI). CytoTox 96® non-radioactive cytotoxicity assay kit (cat # G1782) and DeadEnd Fluorometric TUNEL assay kit (cat # G3250) (Promega, Valencia, CA). Hard Set mounting medium with DAPI (cat # H-1500) (Vector Laboratories, Burlingame, CA). DNase (cat #AM2222), Alexa Fluor 594nm (cat #A11037), Halt™ proteases and phosphatase inhibitor cocktail (cat #78446) (Fisher Scientific, Hampton, NH). Immun-Blot® PVDF membrane for protein blotting (cat # 1620177), Clarity™ Western ECL substrate (BioRAD, Hercules, CA). The antibodies used in this study are included in Table 1 below.

Warns J., Marwarha G., Freking N, & Ghribi O. (2018). 27-hydroxycholesterol decreases cell proliferation in colon cancer cell lines. Biochimie, 153, 171-180.

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