As described in ref. 64 (link), for the implantation of a head-post and optical chamber, the animals were anesthetized with gas anesthesia (Isoflurane 1.5–2.5%; Pfizer) and injected with an antibiotic (Baytril, 0.5 ml, 2%; Bayer Yakuhin), a steroidal anti-inflammatory drug (Dexamethasone; Kyoritsu Seiyaku), an anti-edema agent (Glyceol, 100 μl; Chugai Pharmaceutical) to reduce brain swelling, and a painkiller (Lepetan, Otsuka Pharmaceutical). The scalp and periosteum were retracted, exposing the skull, and then a 5.5 mm diameter trephination was made with a micro drill (Meisinger LLC). Two 5 mm coverslips (120-170 μm thickness) were positioned in the center of the craniotomy in direct contact with the meninges, topped by a 6 mm diameter coverslip with the same thickness. When needed, Gelform (Pfizer) was applied around the 5 mm coverslip to stop any bleeding. The 6-mm coverslip was fixed to the bone with cyanoacrylic glue (Aron Alpha, Toagosei). A round metal chamber (7.1 mm diameter) combined with a head-post was centered on the craniotomy and cemented to the bone with dental adhesive (Super-Bond C&B, Sun Medical), which was mixed with a black dye for improved light absorbance during imaging.
Dexamethasone (dex)
Manufactured by Kyoritsu Seiyaku
Sourced in Japan
Dexamethasone is a synthetic corticosteroid used in laboratory settings. It is a potent anti-inflammatory and immunosuppressant agent. Dexamethasone is commonly used in research applications to study the effects of glucocorticoids on cellular processes and diseases.
Automatically generated - may contain errors
4 protocols using dexamethasone (dex)
1
Cranial window implantation
2
Cranial Implant for Optical Imaging
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Implantation of a head-post and optical chamber. Animals were anesthetized with gas anesthesia (Isoflurane 1.5–2.5%; Pfizer) and injected with an antibiotic (Baytrile, 0.5 ml, 2%; Bayer Yakuhin), a steroidal anti-inflammatory drug (Dexamethasone; Kyoritsu Seiyaku), an anti-edema agent (Glyceol, 100 µl, Chugai Pharmaceutical) to reduce swelling of the brain, and a painkiller (Lepetan, Otsuka Pharmaceutical). The scalp and periosteum were retracted, exposing the skull, then a 4 mm diameter trephination was made with a micro drill (Meisinger LLC). A 4 mm coverslip (120–170 µm thickness) was positioned in the center of the craniotomy in direct contact with the brain, topped by a 6 mm diameter coverslip with the same thickness. When needed, Gelform (Pfizer) was applied around the 4 mm coverslip to stop any bleeding. The 6 mm coverslip was fixed to the bone with cyanoacrylic glue (Aron Alpha, Toagosei). A round metal chamber (6.1 mm diameter) combined with a head-post was centered on the craniotomy and cemented to the bone with dental adhesive (Super-Bond C&B, Sun Medical), mixed to a black dye for improved light absorbance during imaging.
3
Beef Cow Calving Monitoring Protocol
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All experiments were conducted at NILGS, NARO (Nasushiobara, Tochigi, Japan; 36.9 N, 139.9 E) from December 2017 to November 2018. A total 24 parturitions from 24 beef cows were used for
data collection. The tested cows were Japanese Black (JB; n = 18) and crossbred cows (Japanese Black bull × Holstein cows, F1; n = 6) (age: 67.3 ± 33.0 months; body weight: 606.7 ± 115.2 kg;
parity: 2.1 ± 1.9 including 4 primiparous cows; means ± SD). Most of the JB cows were bred by artificial insemination (n = 17); the other JB (n = 1) and all F1 cows (n = 6) were bred by
embryo transfer. Each cow was moved to a maternity pen (4 m × 4 m) by at least 7 days (d) prior to the due date; at the same time, multiple sensors were attached for the collection of ST, VT
and behavioral index data and remained in place until delivery. Cows in the maternity pens were given hay (3.0–4.0 kg) and concentrate (1.0–1.5 kg) twice per day (0900 h and 1600 h) and had
free access to water. Three cows received calving induction treatments (500 mg dexamethasone i.v., 4 mg estradiol i.m. (Kyoritsu Seiyaku, Tokyo, Japan), and 500 µg cloprostenol i.m. (Nippon
Zenyaku Kogyo, Tokyo, Japan)) and seven deliveries were assisted.
data collection. The tested cows were Japanese Black (JB; n = 18) and crossbred cows (Japanese Black bull × Holstein cows, F1; n = 6) (age: 67.3 ± 33.0 months; body weight: 606.7 ± 115.2 kg;
parity: 2.1 ± 1.9 including 4 primiparous cows; means ± SD). Most of the JB cows were bred by artificial insemination (n = 17); the other JB (n = 1) and all F1 cows (n = 6) were bred by
embryo transfer. Each cow was moved to a maternity pen (4 m × 4 m) by at least 7 days (d) prior to the due date; at the same time, multiple sensors were attached for the collection of ST, VT
and behavioral index data and remained in place until delivery. Cows in the maternity pens were given hay (3.0–4.0 kg) and concentrate (1.0–1.5 kg) twice per day (0900 h and 1600 h) and had
free access to water. Three cows received calving induction treatments (500 mg dexamethasone i.v., 4 mg estradiol i.m. (Kyoritsu Seiyaku, Tokyo, Japan), and 500 µg cloprostenol i.m. (Nippon
Zenyaku Kogyo, Tokyo, Japan)) and seven deliveries were assisted.
4
Combination Therapy for Myeloma
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TAS4464 was synthesized at Taiho Pharmaceutical. Bortezomib was purchased from LKT Laboratories. Lenalidomide was purchased from MedChemExpress. Dexamethasone was purchased from Kyoritsu Seiyaku. Daratumumab was purchased from Janssen‐Cilag. Elotuzumab was purchased from Bristol‐Myers Squibb GmbH & Co. KGaA.
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