Special peptone

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom

Special peptone is a complex mixture of amino acids, peptides, and other nitrogenous compounds derived from the enzymatic hydrolysis of proteins. It is commonly used as a nutrient source in microbiological culture media to support the growth of a wide range of microorganisms.

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Lab products found in correlation

Yeast extract, by Thermo Fisher Scientific (2 mentions) Antibiotic antimycotic mix, by Thermo Fisher Scientific (1 mentions) Yeast extract, by Merck Group (1 mentions) Casamino acid, by Merck Group (1 mentions) Trypticase soy agar, by Merck Group (1 mentions) Trypticase soy broth (tsb), by Merck Group (1 mentions) Luria bertani agar, by Thermo Fisher Scientific (1 mentions) S marcescens, by Merck Group (1 mentions) Agar powder, by Thermo Fisher Scientific (1 mentions) Gram staining kit, by Merck Group (1 mentions) Phosphate buffered saline tablets, by Thermo Fisher Scientific (1 mentions) Lab lemco powder, by Thermo Fisher Scientific (1 mentions)

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Peptone (161 citations)

5 protocols using special peptone

Axenic Dictyostelium discoideum Cell Lines

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Axenic Dictyostelium discoideum cell lines expressing Epac1camps (AX4 background, gift of Dr. Satoshi Sawai), Epac1camps and mRFPmars (AX4 background), ECFP, and EYFP (both AX3 background, gifts of Dr. Carole Parent) were grown according to standard protocols (Fey et al, 2007 (link)). Briefly, vegetative cells were grown at 22°C while shaking at 180 rpm in PS medium consisting of 1.0% special peptone (Oxoid), 0.7% yeast extract (Oxoid), 1.5% D-glucose, 0.14% KH₂PO₄, 0.012% Na₂HPO₄-7H₂O, 40 ng/ml vitamin B12, 80 ng/ml folic acid, and 1× antibiotic-antimycotic mix (Gibco) supplemented with 5 μg/ml (EYFP/AX3), 10 μg/ml (Epac1camps/AX4), or 20 μg/ml (ECFP/AX3) G418. Vegetative cells were washed and shaken at 1–2 × 10⁷ cells/ml in development buffer (10 mM K/Na₂ phosphate buffer, 2 mM MgSO₄, and 200 μM CaCl₂, pH 6.5) for 4–5 h prior to experiments.
The expression vector pBSRH-mars, which permits constitutive expression of mRFPmars in Dictyostelium discoideum under control of the act15 promoter, was kindly provided by Dr. Robert Cooper. The Epac1camps strain was transformed with pBSRH-mars by electroporation following a standard protocol (Gaudet et al, 2007 (link)), and a clone was selected based on fluorescence intensity. Fluorescence intensity of mRFPmars appears uniform in the cytosol.

Sgro A.E., Schwab D.J., Noorbakhsh J., Mestler T., Mehta P, & Gregor T. (2015). From intracellular signaling to population oscillations: bridging size- and time-scales in collective behavior. Molecular Systems Biology, 11(1), 779.

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Endospore-Forming Bacteria Antagonism Assay

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Aerobic Endospore Forming Bacteria (AEFB) isolated from Musa sp. plants (Humboldt Institute Collection No. 191), B. subtilis NCIB 3610 (wild type, WT), B. subtilis SMY, B. subtilis PY79 and knockout strains of NCIB 3610 (Supplementary Tables S1 and S3) were used in the different inducible antagonism trials. These strains were stored in TSB (trypticase soy broth, Merck, Germany) with 20% v/v glycerol at −80 °C and activated in TSA (trypticase soy agar, Merck) or LB agar (Luria Bertani Agar, Basingstoke, Oxoid, England) for 48 h at 30 °C before use. Strains R. solanacearum EAP-009 (GenBank accession N° KU603426), Serratia marcescens EAD-005 (GenBank accession N° KU603427)^{45 (link)} and R. solanacearum AW1^{46 (link)}, all were stored in BG medium⁴⁷ plus 20% v/v glycerol at −80 °C and activated at 30 °C for 72 h in BGA medium (BG plus 18 g/L agar (BD, Ontario, Canada)). BG medium was composed of 10 g/L special peptone (Oxoid), 1 g/L casamino acids (BD), 1 g/L yeast extract (Merck) and 5 g/L glucose (Merck). Pseudomonas putida UA-0095, Xanthomonas sp. UA-1539, B. cepacea UA-1541, S. marcescens UA-1538, Salmonella sp. ATCC 14028, Staphylococcus sp. G and E. coli DH5α (Supplementary Table S1) were stored in TSB (trypticase soy broth, Merck) with 20% v/v glycerol at −80 °C and activated in TSA or LB agar for 48 h at 30 °C before use.

Sierra-Zapata L., Álvarez J.C., Romero-Tabarez M., Silby M.W., Traxler M.F., Behie S.W., Pessotti R.D, & Villegas-Escobar V. (2020). Inducible Antibacterial Activity in the Bacillales by Triphenyl Tetrazolium Chloride. Scientific Reports, 10, 5563.

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Western Blot Analysis of FLAG-pqsA Expression

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For western blot analysis, cultures were grown in PS:DB media, which consists of DB and 10% (v/v) PS medium (10 g L⁻¹ Special Peptone (Oxoid), 7 g L⁻¹ Yeast Extract (Oxoid), 10 mM KH₂PO₄, 0.45 M Na₂HPO₄, 15 g L⁻¹ glucose, 20 nM vitamin B12, 180 nM Folic Acid, pH 6.5). Overnight cultures of FLAG-pqsA were diluted 1:100 in PS:DB and grown to OD_600nm = 0.5–0.6 at 37 °C with shaking (250 rpm). Cultures were transferred to 22 × 22 cm culture dishes and incubated for 1 h at 37 °C on an orbital shaker (80 rpm). Planktonic samples were isolated by aspirating the culture media. Surface-attached cells were washed twice in PBS and removed from the surface using a cell scraper. Cells were resuspended in loading buffer to equal concentrations, based on OD_600nm, and seperated on a 10% SDS-PAGE. Blots were probed using DYKDDDDK Tag (D6W5B) Rabbit monoclonal antibody (14793 S, Cell Signaling Technologies, Danvers, MA) at a 1:1000 dilution and a polyclonal antibody targeting the σ⁷⁰ subunit of RNA polymerase, provided by the Bassler lab, at 1:5000 dilution. Primary antibody was detected with anti-rabbit IgG, HRP-linked secondary antibody (7074 S, Cell Signaling Technologies, Danvers, MA) at 1:3000 dilution.

Chuang S.K., Vrla G.D., Fröhlich K.S, & Gitai Z. (2019). Surface association sensitizes Pseudomonas aeruginosa to quorum sensing. Nature Communications, 10, 4118.

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Optimizing Bifidobacterium growth with prebiotic supplements

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Modified Bifidobacterium broth (1000 mL) with pH 6.5 ± 0.2 was prepared using peptone special (22.2 g), NaCl (4.8 g), and L-cysteine hydrochloride monohydrate (0.5 g) with prebiotics and sugars. Four different sugars (fructose, sucrose, glucose, and lactose) at different concentrations (1, 2, 3, and 4%) with FOS supplementation at different concentrations (0.5, 1, 2, 3, and 4%) were used for growth and survival assays. Agar powder, phosphate-buffered saline (PBS) tablets, anaerogens, peptone special, sodium chloride (NaCl), and L-cysteine hydrochloride monohydrate were purchased from Oxoid, UK. Glucose, fructose, sucrose, lactose, and gram staining kit were purchased from Sigma-Aldrich, USA. Fructooligosaccharide (FOS) was obtained from Fiatec Biosystems Sdn. Bhd, Malaysia, with a degree of polymerization between 3 and 8.

Parhi P., Song K.P, & Choo W.S. (2022). Growth and survival of Bifidobacterium breve and Bifidobacterium longum in various sugar systems with fructooligosaccharide supplementation. Journal of Food Science and Technology, 59(10), 3775-3786.

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Evaluating Anti-tuberculosis Activity

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To determine the biological activity of substances 1–5 possessing anti-tuberculosis properties in the M. smegmatis mc²155 test system, the paper disk method was used. The technique involved determining the size of the zone of inhibition of the growth of the strain seeded as a lawn on an agar medium, around paper disks containing the compound in various concentrations. The bacteria washed off Petri dishes with tryptone soya agar M-290 medium (Himedia) were grown overnight in Lemco-TW liquid medium (Lab Lemco' Powder 5 g L⁻¹ (Oxoid), peptone special 5 g L⁻¹ (Oxoid), NaCl 5 g L⁻¹, Tween-80) at +37 °C until the average logarithmic growth phase at optical density OD600 = 1.5, then mixed with molten agar medium M-290 in a ratio of 1 : 9 : 10 (culture : Lemco-TW : M-290) and the resulting mixture was poured as a top layer onto Petri dishes, 5 ml per dish, with 20 ml already solidified M-290 agar medium. After the agar in the top layer solidified, paper disks soaked with a solution of the test substance were placed on the plate surface. The culture was incubated for 24 hours at +37 °C. The diameter of the zone of inhibition of M. smegmatis mc²155 growth around the paper disk impregnated with the compound was determined. The MIC (minimum inhibiting concentration) was taken as the concentration of the compound where the zone of growth inhibition was the smallest.

Lutsenko I.A., Baravikov D.E., Koshenskova K.A., Kiskin M.A., Nelyubina Y.V., Primakov P.V., Voronina Y.K., Garaeva V.V., Aleshin D.A., Aliev T.M., Danilenko V.N., Bekker O.B, & Eremenko I.L. (2022). What are the prospects for using complexes of copper(ii) and zinc(ii) to suppress the vital activity of Mycolicibacterium smegmatis?. RSC Advances, 12(9), 5173-5183.

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