Mygliol 812

Sorbitan monostearate 80 (Span 80^®), dexamethasone, and complete Freund’s adjuvant (CFA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Polysorbate 80 (Tween 80^®) was provided by Vetec Química Fina Ltd. (Rio de Janeiro, Brazil). Mygliol 812^® was acquired from Sasol (Marl, Germany). The ELISA kit for interleukin (IL)-1β was acquired from R&D Systems (Minneapolis, MN, USA). Diagnostic kits for biochemical analyses were acquired from Labtest Diagnóstica (Lagoa Santa, Brazil). Sodium phosphate monobasic and dibasic (components of sodium phosphate buffer) were purchased from Sigma-Aldrich Co. (São Paulo, Brazil).

CV oil (5-isopropryl-2-methylphenol, purity 98%) and sorbitan monostearate 80 (Span 80^®) were purchased from Sigma-Aldrich (São Paulo, Brazil). Polysorbate 80 (Tween 80^®) was provided by Vetec Química Fina LTDA (Duque de Caxias, Rio de Janeiro, Brazil). MCTs (Mygliol 812^®) were acquired from Sasol (Brunsbüttel, Germany). Ethanol was sourced from Merck (São Paulo, Brazil), and dimethyl sulfoxide (DMSO) was purchased from NEON (Suzano, São Paulo, Brazil). The anti-CD3 and anti-CD28 antibodies were purchased from Ebiosciences (San Diego, CA, USA), Ficoll Paque Plus was purchased from GE Healthcare Biosciences (Pittsburgh, PA, USA), and the media were purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). The human ELISA kits were provided by BD Biosciences (USA, for INF-γ, TNF-α, IL-6, and IL-10), eBioscience (USA, for IL-2 and IL-17), and Invitrogen (USA for IL-1β).

Carvacrol oil (5-isopropryl-2-methylphenol, purity 98%), sorbitan monostearate 80 (Span 80^®), and Tiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, United States). Polysorbate 80 (Tween 80^®) was provided by Vetec Química Fina LTDA (Duque de Caxias, RJ, Brazil). Medium chain triglyceride (Mygliol 812^®) was acquired from Sasol (Brunsbüttel, Germany). RPMI 1,640 culture medium Dulbecco’s Modifed Eagle Medium (DMEM), inactivated fetal bovine serum and antibiotics (10,000 units/ml Penicillin G sodium salt and 10 mg/ml streptomycin sulfate) were purchased from Vitrocell (Campinas, SP, Brazil). Praziquantel was kindly provided by Ecovet Indústria Veterinária Ltda (São Paulo, Brazil).