Agilent 5975n

Analyses were performed with an Agilent 5975N (Agilent Technologies) using MS/FID (Agilent MSD5975) detector, a 100% polydimethylsiloxane (Agilent VF-1MS) (0.25 µm × 0.25 mm × 60 m) as the stationary phase, with Helium as the mobile phase, over 95 min, 1 mL/min, column temperature: 250 °C, sample volume injection: 0.5 μL.

The A. lavandulaefolia essential oil composition was analyzed by gas chromatography using a GC system (Agilent 6890N, Agilent Technologies Incorporated, Santa Clara, CA, USA). An HP-1 capillary column (30 m × 0.25 mm × 0.25 μm film thickness) was used with injection in split-ratio mode (40:1). The oven temperature started at 60 °C for 3 min, and then it was increased to 100 °C (10 °C/min). Afterward, it was increased to 180 °C (5 °C/min) and subsequently increased to 280 °C (15 °C/min), and remained for 5 min. The carrier gas was helium at a flow rate of 1.0 mL/min, and the injection volume used in each analysis was 0.5 μL.
The mass spectrometer (Agilent 5975N, Agilent Technologies Incorporated, Santa Clara, CA, USA) used an electron ionization source with 70 eV ionization energy. The ion source temperature was 230 °C, with a scanning range between 20 and 800 m/z. The temperature of the quadrupole was 150 °C, and the mass spectrum acquisition delay time was 4 min. The constituents were identified based on their retention index and confirmed by the use of mass spectral libraries (National Institute of Standards and Technology, NIST databases). In addition, the major oil compounds were identified by co-injection with standards. The relative concentration of each component in the oil was quantified by a peak area normalization method integrated into the analysis.

The constituents of the A. lavandulaefolia essential oil were confirmed by gas chromatography using a GC system (Agilent 6890N, Agilent Technologies Incorporated, Santa Clara, CA, USA), which was equipped with an HP-1 capillary column (30 m × 0.25 mm × 0.25 μm film thickness). The oven temperature was programmed at 60 °C for 3 min, with an increase of 10 °C/min until 280 °C for 5 min. The carrier gas was helium at a flow rate of 1.0 mL/min, the split ratio was 50:1, and the injection volume was 1.0 μL.
The mass spectrometer (Agilent 5975N, Agilent Technologies Incorporated, Santa Clara, CA, USA) used an electron ionization source with 70 eV ionization energy. The ion source temperature was 230 °C, with a scanning range between 20 and 650 m/z. The temperature of the quadrupole was 150 °C, and the mass spectrum acquisition delay time was 2 min. The constituents were identified based on their retention index and by the use of the mass spectral libraries (National Institute of Standards and Technology, Nist-2008). The area normalization method was used to calculate the relative content of each constituent.