Analyzer feed system

Manufactured by Merck Group

The Analyzer Feed System is a lab equipment product that is designed to automate and streamline the sample introduction process for analytical instruments. It provides a controlled and consistent method for feeding samples into the analyzer, ensuring reliable and reproducible results.

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Lab products found in correlation

Lc 20at pump, by Shimadzu (7 mentions) Microflex spectrometer, by Bruker (7 mentions) 6230 tof lc ms spectrometer, by Agilent Technologies (7 mentions) Sil 20a autosampler, by Shimadzu (7 mentions) Cbm 20a communications bus module, by Shimadzu (7 mentions) Spd 20a, by Shimadzu (5 mentions) Synergy 2, by Agilent Technologies (1 mentions) Spd 20a uv vis detector, by Shimadzu (1 mentions)

9 protocols using analyzer feed system

Chromatographic Analysis of Organic Compounds

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All chemical reagents were purchased from commercial sources (Alfa-Aesar, Sigma-Aldrich, and Acros) and used without further purification. Solvents were purchased from commercial sources (Sigma-Aldrich and J.T. Baker) and used as obtained. Water (18 MΩ) was purified using a Millipore Analyzer Feed System. Solid-phase resin was purchased from NovaBiochem.
Reversed-phase high performance liquid chromatography (RP-HPLC) was performed using a Shimadzu system equipped with an SCL-10Avp controller, an LC-10AT pump, an FCV-10ALvp solvent mixer, and an SPD-10MAvp UV/vis diode array detector. MALDI-TOF mass spectrometry (MS) data were obtained on a Bruker RELEX II spectrometer equipped with a 337 nm laser and a reflectron. In positive ion mode, the acceleration voltage was 25 kV. Exact mass (EM) data were obtained on a Waters (Micromass) LCT ESI-TOF spectrometer. The samples were sprayed with a sample cone voltage of 20 V.

Tal-Gan Y., Ivancic M., Cornilescu G, & Blackwell H.E. (2015). Characterization of Structural Elements in Native Autoinducing Peptides and Non-Native Analogues that Permit the Differential Modulation of AgrC-type Quorum Sensing Receptors in Staphylococcus aureus. Organic & biomolecular chemistry, 14(1), 113-121.

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Standardized Bacterial Assay Protocol

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All standard
biological reagents were purchased from Sigma-Aldrich or Gold Biotechnology
and used according to enclosed instructions. Buffers and solutions
for Miller absorbance assays in E. coli (Z buffer,
0.1% aqueous SDS, and phosphate buffer) were prepared as described
previously.⁴¹ Water (18 MΩ) was purified
using a Millipore Analyzer Feed System.
The bacterial strains
and plasmids used in this study are listed in Table S1. Bacteria were grown in a standard laboratory incubator
at 37 °C with shaking (200 rpm) in Luria–Bertani (LB)
medium unless otherwise noted. Absorbance and fluorescence measurements
were obtained using a Biotek Synergy 2 microplate reader using Gen5
1.05 data analysis software. All biological assays were performed
in triplicate. EC₅₀ and IC₅₀ values, as well
as respective 95% confidence intervals, were calculated using GraphPad
Prism software (v. 4.0) using a sigmoidal curve fit (see Supplementary Note S6 for more information regarding
curve fitting).

Moore J.D., Rossi F.M., Welsh M.A., Nyffeler K.E, & Blackwell H.E. (2015). A Comparative Analysis of Synthetic Quorum Sensing Modulators in Pseudomonas aeruginosa: New Insights into Mechanism, Active Efflux Susceptibility, Phenotypic Response, and Next-Generation Ligand Design. Journal of the American Chemical Society, 137(46), 14626-14639.

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Characterization of Organic Compounds

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All chemical reagents and solvents were purchased from Sigma-Aldrich and used without further purification. Water (18 MΩ) was purified using a Millipore Analyzer Feed System. Solid-phase resins were purchased from Advanced ChemTech and Chem-Impex International.
Reversed-phase high-performance liquid chromatography (RP-HPLC) was performed using a Shimadzu system equipped with a CBM-20A communications bus module, two LC-20AT pumps, an SIL-20A auto sampler, an SPD-20A UV/vis detector, a CTO-20A column oven, and an FRC-10A fraction collector. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) data were obtained on a Bruker Microflex spectrometer equipped with a 60 Hz nitrogen laser and a reflectron. In positive ion mode, the acceleration voltage on Ion Source 1 was 19.01 kV. Exact mass (EM) data were obtained on an Agilent Technologies 6230 TOF LC/MS spectrometer. The samples were sprayed with a capillary voltage of 3500 V, and the electrospray ionization (ESI) source parameters were as follows: gas temperature of 325 °C at a drying gas flow rate of 8 L/ min at a pressure of 35 psi.

Bikash C.R., Hamry S.R, & Tal-Gan Y. (2018). Structure–Activity Relationships (SARs) of the Competence Stimulating Peptide (CSP) in Streptococcus mutans Reveal Motifs Critical for Membrane Protease SepM Recognition and ComD Receptor Activation. ACS infectious diseases, 4(9), 1385-1394.

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Characterization of Organic Compounds

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All the chemical reagents and solvents were purchased from Sigma-Aldrich or Chem-Impex and used without further purification. Water (18 MΩ) was purified using a Millipore Analyzer Feed System. Solid-phase resins were purchased from Advanced Chem Tech.
Reversed-phase high-performance liquid chromatography (RP-HPLC) was performed using a Shimadzu system equipped with a CBM-20A communications bus module, two LC-20AT pumps, an SIL-20A auto sampler, an SPD-20A UV/vis detector, a CTO-20A column oven, and an FRC-10A fraction collector. The matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) data were obtained using a Bruker Microflex spectrometer equipped with a 60 Hz nitrogen laser and a reflectron. In positive ion mode, the acceleration voltage on Ion Source 1 was 19.01 kV. The exact mass (EM) data were obtained using an Agilent Technologies 6230 TOF LC/MS spectrometer. The samples were sprayed with a capillary voltage of 3500 V and the electrospray ionization (ESI) source parameters were as follows: gas temperature of 325 °C at a drying gas flow rate of 8 L min⁻¹ at a pressure of 35 psi.

Milly T.A, & Tal-Gan Y. (2020). Biological evaluation of native streptococcal competence stimulating peptides reveals potential crosstalk between Streptococcus mitis and Streptococcus pneumoniae and a new scaffold for the development of S. pneumoniae quorum sensing modulators. RSC Chemical Biology, 1(2), 60-67.

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Optimized HPLC-MS Analysis of Organic Compounds

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All chemical reagents and solvents were purchased from Sigma-Aldrich and used without further purification. Water (18 MΩ) was purified using a Millipore Analyzer Feed System. Solid-phase resins were purchased from Advanced ChemTech. Reversed-phase high-performance liquid chromatography (RP-HPLC) was performed using a Shimadzu system equipped with a CBM-20A communications bus module, two LC-20AT pumps, an SIL-20A auto sampler, an SPD-20A UV/VIS detector, a CTO-20A column oven, and an FRC-10A fraction collector. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) data were obtained on a Bruker Microflex spectrometer equipped with a 60 Hz nitrogen laser and a reflectron. In positive ion mode, the acceleration voltage on Ion Source 1 was 19.01 kV. Exact mass (EM) data were obtained on an Agilent Technologies 6230 TOF LC/MS spectrometer. The samples were sprayed with a capillary voltage of 3500 V and the electrospray ionization (ESI) source parameters were as follows: gas temperature of 325 °C at a drying gas flow rate of 3 L/min at a pressure of 25 psi.

Koirala B., Lin J., Lau G.W, & Tal-Gan Y. (2018). Development of a Dominant Negative Competence Stimulating Peptide (dnCSP) that Attenuates Streptococcus pneumoniae Infectivity in a Mouse Model of Acute Pneumonia. Chembiochem : a European journal of chemical biology, 19(22), 2380-2386.

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Purification and Characterization of Compounds

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All chemical reagents and solvents were purchased from Sigma-Aldrich or Chem-Impex and used without further purification. Water (18 MΩ) was purified using a Millipore Analyzer Feed System. Solid-phase resin was purchased from Advanced ChemTech.
Reversed-phase high-performance liquid chromatography (RP-HPLC) was performed using a Shimadzu system equipped with a CBM-20A communications bus module, two LC-20AT pumps, an SIL-20A auto sampler, an SPD-20A UV/VIS detector, a CTO-20A column oven, and an FRC-10A fraction collector. All RP-HPLC solvents (18 MΩ water and HPLC-grade acetonitrile (ACN)) contained 0.1% trifluoroacetic acid (TFA). Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) data were obtained on a Bruker Microflex spectrometer equipped with a 60 Hz nitrogen laser and a reflectron. In positive ion mode, the acceleration voltage on Ion Source 1 was 19.01 kV. Exact mass (EM) data were obtained on an Agilent Technologies 6230 TOF LC/MS spectrometer. The samples were sprayed with a capillary voltage of 3500 V and the electrospray ionization (ESI) source parameters were as follows: gas temperature of 325 °C at a drying gas flow rate of 8 L/min at a pressure of 35 psi.

McBrayer D.N., Ghosh U., Lella M., Cameron C.D, & Tal-Gan Y. (2022). Peptoid-Peptide Hybrid Analogs of the Enterococcus faecalis Fsr Auto-Inducing Peptide (AIP) Reveal Crucial Structure-Activity Relationships. Chembiochem : a European journal of chemical biology, 24(1), e202200527.

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Purification and Characterization of Peptides

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All chemical reagents and solvents were purchased from Sigma-Aldrich and used without further purification. Amino acids were purchased from Advanced ChemTech. Water (18 MΩ) was purified using a Millipore Analyzer Feed System. Solid-phase resins were purchased from Advanced ChemTech and Chem-Impex International.
Reversed-phase high-performance liquid chromatography (RP-HPLC) was performed using a Shimadzu system equipped with a CBM-20A communications bus module, two LC-20AT pumps, an SIL-20A auto sampler, an SPD-20A UV/vis detector, a CTO-20A column oven, and an FRC-10A fraction collector. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) data were obtained on a Bruker Microflex spectrometer equipped with a 60 Hz nitrogen laser and a reflectron. In positive ion mode, the acceleration voltage on Ion Source 1 was 19.01 kV. Exact mass (EM) data were obtained on an Agilent Technologies 6230 TOF LC/MS spectrometer. The samples were sprayed with a capillary voltage of 3500 V, and the electrospray ionization (ESI) source parameters were as follows: gas temperature of 325 °C at a drying gas flow rate of 8 L/ min at a pressure of 35 psi.

Bikash C.R, & Tal-Gan Y. (2020). Structure Activity Relationship Study of the XIP Quorum Sensing Pheromone in Streptococcus mutans Reveal Inhibitors of the Competence Regulon. ACS chemical biology, 15(10), 2833-2841.

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Purification and Characterization of Biomolecules

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All chemical reagents and solvents were purchased from Sigma-Aldrich or Chem-Impex and used without further purification. Water (18 MΩ) was purified using a Millipore Analyzer Feed System. Solid-phase resins were purchased from Advanced Chem Tech.
Reversed-phase high-performance liquid chromatography (RP-HPLC) was performed using a Shimadzu system equipped with a CBM-20A communications bus module, two LC-20AT pumps, an SIL-20A auto sampler, an SPD-20A UV/VIS detector, a CTO-20A column oven, and an FRC-10A fraction collector. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) data were obtained on a Bruker Microflex spectrometer equipped with a 60 Hz nitrogen laser and a reflectron. In positive ion mode, the acceleration voltage on Ion Source 1 was 19.01 kV. Exact mass (EM) data were obtained on an Agilent Technologies 6230 TOF LC/MS spectrometer. The samples were sprayed with a capillary voltage of 3500 V and the electrospray ionization (ESI) source parameters were as follows: gas temperature of 325 °C at a drying gas flow rate of 8 L/min at a pressure of 35 psi.

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Analytical Characterization of Synthetic Compounds

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All chemical reagents and solvents were purchased from Sigma-Aldrich and used without further purification. Water (18 MΩ) was purified using a Millipore Analyzer Feed System. Solid-phase resins were purchased from Advanced ChemTech and Chem-Impex International.
Reversed-phase high-performance liquid chromatography (RP-HPLC) was performed using a Shimadzu system equipped with a CBM-20A communications bus module, two LC-20AT pumps, an SIL-20A auto sampler, an SPD-20A UV/VIS detector, a CTO-20A column oven, and an FRC-10A fraction collector. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) data were obtained on a Bruker Microflex spectrometer equipped with a 60 Hz nitrogen laser and a reflectron. In positive ion mode, the acceleration voltage on Ion Source 1 was 19.01 kV. Exact mass (EM) data were obtained on an Agilent Technologies 6230 TOF LC/MS spectrometer. The samples were sprayed with a capillary voltage of 3500 V and the electrospray ionization (ESI) source parameters were as follows: gas temperature of 325 °C at a drying gas flow rate of 8 L/min at a pressure of 35 psi.

Yang Y., Koirala B., Sanchez L.A., Phillips N.R., Hamry S.R, & Tal-Gan Y. (2017). Structure–Activity Relationships of the Competence Stimulating Peptides (CSPs) in Streptococcus pneumoniae Reveal Motifs Critical for intra-group and cross-group ComD Receptor Activation. ACS chemical biology, 12(4), 1141-1151.

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