Pituitary-derived TSH, Luteinizing hormone (LH), Follicle stimulating hormone (FSH) and Adrenocorticotropic hormone (ACTH) were purchased from Sigma–Aldrich (St. Louis, USA). Recombinant TSH was purchased from R&D systems (Minneapolis, USA). Tritium labelled radioactive progesterone and testosterone were purchased from Perkin Elmer (Waltham, USA). Both the mouse monoclonal testosterone antibody (clone 4E1G2) and rabbit polyclonal progesterone antibody were purchased from Bio-Rad (Hercules, USA). Primers were purchased from Sigma–Aldrich (St. Louis, USA). Rabbit anti-StAR antibody and rabbit anti-CYP11A1 antibody were purchased from Cell Signalling Technologies (Boston, USA). Rabbit anti-3β-hydroxy steroid dehydrogenase-1 (3BHSD-1) antibody and rabbit anti-TSH receptor antibody were purchased from Abcam (Cambridge, UK). Anti-rabbit HRP labelled secondary antibody was purchased from Cell Signalling Technologies (Boston, USA).
Adrenocorticotropic hormone acth
Manufactured by Merck Group
Sourced in United States, Switzerland
Adrenocorticotropic hormone (ACTH) is a peptide hormone produced and secreted by the anterior pituitary gland. Its primary function is to stimulate the adrenal cortex to produce and release corticosteroid hormones, including cortisol, which play a crucial role in the body's stress response and metabolic processes.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit polyclonal progesterone antibody, by Bio-Rad (1 mentions)
Testosterone, by PerkinElmer (1 mentions)
Luteinizing hormone lh, by Merck Group (1 mentions)
Follicle stimulating hormone fsh, by Merck Group (1 mentions)
Mouse monoclonal testosterone antibody clone 4e1g2, by Bio-Rad (1 mentions)
Tritium labelled radioactive progesterone, by PerkinElmer (1 mentions)
Cb17 scid mice, by Charles River Laboratories (1 mentions)
3 isobutyl 1 methylxanthine ibmx, by Cayman Chemical (1 mentions)
Htrf camp dynamic kit, by PerkinElmer (1 mentions)
2 protocols using adrenocorticotropic hormone acth
1
Steroidogenic Pathway Characterization
2
Teratoma Formation and cAMP Assay in iPSC Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The iPSCs cells (1 3 10 6 cells in 20-mL PBS) were injected into the testis of 8-to 10-week-old male CB17 SCID mice (Charles River). Teratomas were resected 9-12 weeks after injection, fixed with 10% neutralbuffered formalin for 24 h, and paraffin-embedded sections (4 mm) were processed and stained with hematoxylin and eosin. All mouse studies were carried out according to protocols approved by the Animal Research Committee of Tokyo Dental College (No. 270401).
cAMP assay cAMP accumulation was measured using homogeneous time-resolved fluorescence (HTRF) cAMP dynamic kits (CisBio, Codolet, France) according to the manufacturer's protocol. iPSCs were cultured in 96-well plates (20,000 cells/well) and treated with parathyroid hormone (PTH) (100 nM; Sigma-Aldrich, Buchs, Switzerland) 24 or adrenocorticotropic hormone (ACTH) (10 nM; Sigma-Aldrich, Buchs, Switzerland) 25 along with 500-nM 3-isobutyl-1-methylxanthine (IBMX; Cayman Chemical, Ann Arbor, MI, USA). At each time point, the cells were lysed with 1% Triton X-100. cAMP accumulation was detected by HTRF (l ex = 330 nm, l em = 665 and 620 nm) using a microplate reader (Synergy, BioTek, Winooski, VT, USA).
cAMP assay cAMP accumulation was measured using homogeneous time-resolved fluorescence (HTRF) cAMP dynamic kits (CisBio, Codolet, France) according to the manufacturer's protocol. iPSCs were cultured in 96-well plates (20,000 cells/well) and treated with parathyroid hormone (PTH) (100 nM; Sigma-Aldrich, Buchs, Switzerland) 24 or adrenocorticotropic hormone (ACTH) (10 nM; Sigma-Aldrich, Buchs, Switzerland) 25 along with 500-nM 3-isobutyl-1-methylxanthine (IBMX; Cayman Chemical, Ann Arbor, MI, USA). At each time point, the cells were lysed with 1% Triton X-100. cAMP accumulation was detected by HTRF (l ex = 330 nm, l em = 665 and 620 nm) using a microplate reader (Synergy, BioTek, Winooski, VT, USA).
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