Mouse anti myc monoclonal antibody 9e10 sc 40 and sc 40 af647

The pLifeact-tdTomato construct was kindly provided by Weiping Han (Laboratory of Metabolic Medicine, Singapore). Human insulin, insulin from bovine pancreas, dexamethasone, 3-isobutyl-1-methylxanthine, wortmannin, SB203518, dorsomorphin (compound C), polyphenon 60 from green tea (PP60), HEPES, CaCl₂, NaCl, KCl, MgSO₄ and KH₂PO₄ were purchased from Sigma-Aldrich (Schnelldorf, Germany). Mouse anti-myc monoclonal antibody (9E10: sc-40 and sc-40 AF647) was from Santa Cruz biotechnology (Santa Cruz, CA). Bilberry (BIL, Vaccinium myrtillus) extract was provided by Vitalingo (Berlin, Germany), ginger (GIN, Zingiber officinale) extract by Hawlik Gesundheitsprodukte (Strasslach, Germany), Tindora (TIN, Coccinia grandis) extract by SHAG psoriasisEX (Berlin, Germany), milk thistle extract (MT, Silybum marianum) by McVital (Newark, USA), purslane (PUR, Portulaca oleracea) extract by Frutarom (Portusana^® EFLA308, Haifa, Israel), and jiaogulan (JIA, Gynostemma pentaphyllum) extract by Vitabay (Kerkrade, Netherlands). For preparation of stock solutions, the herbal compounds were dissolved in Krebs Ringer Phosphate HEPES buffer (KRPH; 20 mM HEPES, 1 mM CaCl₂, 136 mM NaCl, 4.7 mM KCl, 1 mM MgSO₄ and 5 mM KH₂PO₄) (PUR and PP60) or in 50% DMSO and 50% KRPH buffer (TIN, GIN, BIL, JIA, MT).