Male Sprague-Dawley rats (10 weeks old, n=15) were divided into three groups; Control group without prostatic inflammation (n=5), Placebo group with prostatic inflammation and placebo treatment (n=5) and Dutasteride group with prostatic inflammation and Dutasteride treatment (n=5). Rats were housed in plastic cages with soft bedding and free access to food and water under a 12/12 hour reversed light-dark cycle. Control rats were injected with 50 μl normal saline into each of bilateral ventral lobes of the prostate as sham operation. Prostatic inflammation was induced by 5% formalin solution injection at a volume of 50μl into each of bilateral ventral lobes of the prostate. Dutasteride group rats were treated with Dutasteride (Sigma-Aldrich Co.), which was dissolved in propyl methyl cellulose (Wako Pure Chemical Industries, Ltd.) at a dose of 0.5mg/kg daily from 2 days before intraprostatic formalin injection whereas Placebo rats received hydroxy propyl methyl cellulose (HPMC) only as vehicle treatment. After cystometry, bladder and ventral lobes of the prostate were harvested for evaluation of mRNA expression and histological analysis.
Dutasteride
Manufactured by Merck Group
Sourced in United States
Dutasteride is a synthetic compound used in laboratory research and development. It functions as a dual 5α-reductase inhibitor, which is an enzyme involved in the conversion of testosterone to dihydrotestosterone. This compound is commonly used in studies exploring its potential applications, but a detailed description of its intended use cannot be provided while maintaining an unbiased and purely factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Finasteride, by Merck Group (3 mentions)
Lipopolysaccharide, by Merck Group (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Diclofenac sodium, by Merck Group (1 mentions)
Testosterone propionate, by Merck Group (1 mentions)
Quercetin dihydrate, by Merck Group (1 mentions)
Crystal violet, by Merck Group (1 mentions)
Streptococcus equi hyaluronic acid, by Merck Group (1 mentions)
Ursolic acid, by Merck Group (1 mentions)
Dmem high glucose, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
Hyaluronidase, by Merck Group (1 mentions)
Cetyltrimethylammonium bromide, by Merck Group (1 mentions)
Propyl methyl cellulose, by Fujifilm (1 mentions)
Androsterone, by Merck Group (1 mentions)
Androstenedione, by Merck Group (1 mentions)
Gestodene, by Merck Group (1 mentions)
Androstanedione, by Merck Group (1 mentions)
Drospirenone, by Merck Group (1 mentions)
11 protocols using dutasteride
1
Dutasteride Modulates Prostatic Inflammation
2
Compound Dilution Protocol for Drug Treatments
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Dutasteride and anastrozole were obtained from Sigma-Aldrich (St. Louis, MO, USA). Finally, ASP9521 was obtained from Adooq bioscience (Irvine, CA, USA). All drugs were diluted in dimetil-sulfoxide (DMSO) (Sigma Aldrich, St. Louis, MO, USA) to start from an initial concentration of 20 mM, 34 mM and 30 mM for Dutasteride, anastrozole and ASP9521, respectively. Then, drugs were diluted again to achieve a work concentration of 10 mM and 1 mM for all treatments.
3
Cellular Assays with Biochemical Compounds
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Dulbecco’s Modified Eagle’s Medium F12 HAM (DMEM/F12), Dulbecco’s Modified Eagle’s Medium with 4500 mg/L glucose (DMEM high glucose), foetal bovine serum (FBS), phosphate-buffered saline (PBS), crystal violet, formaldehyde, lipopolysaccharide (LPS), DMSO, albumin from bovine serum (BSA): fraction V ≥ 98% (A3294), hyaluronidase from bovine testes type I-S, Streptococcus equi hyaluronic acid (HA), cetyltrimethylammonium bromide (CTAB), quercetin dihydrate, ursolic acid (other terpenoids were obtained by isolation), diclofenac sodium, dutasteride, and testosterone propionate were obtained from Sigma-Aldrich (Seelze, Germany). Acetate buffer pH 4.5 was purchased from J.T. Baker Chemical Co. (Phillipsburg, NJ, USA).
4
Mouse Islet Hormone Modulation
5
Steroid Compound Acquisition and Characterization
6
Antibody Sources for Stem Cell Markers
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Monoclonal antibodies against HSD17B2 (#TA504616), HSD17B3 (#CF811500), SHBG (#TA507187), and SRD5A1 (#PA5-75675) were obtained from Thermo Fisher Scientific Inc. (Bartlesville, OK, USA), while KLF4 (#12173), OCT4A (#2840), MRP1/ABCC1 (#14685), MDR1/ABCB1 (#13342), and ALDH1 (#36671) were all purchased from Cell Signaling Technology (CST, Beverly, MA, USA), and GAPDH (#sc-32233) was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Dutasteride (#SML1221, ≥98% (HPLC)) was purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA).
7
Recombinant Human AKR1D1 Inhibition Assay
8
Dutasteride Neuroprotection in MPTP-Induced Parkinson's Model
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Mice that received dutasteride (Toronto Research Chemicals, Toronto, ON, Canada) (5 mg/kg, intraperitoneal) or vehicle only (0.9% saline with 1% tween 80, i.p.) were injected once daily for 10 days. On the fifth day of dutasteride/vehicle treatment, mice received four intraperitoneal injections (5.5 mg/kg) at 2-h intervals of acute treatment with MPTP (Sigma Chemical, St. Louis, MO, USA) or saline (Figure 6 ). The MPTP dose of the present study was chosen to produce a partial lesion of the DA nigrostriatal pathway as reported for these SHAM male mice [55 (link)] in order to model early stages of PD in humans. dutasteride was investigated in the present study at a dose that protected against MPTP toxicity brain DA markers of male mice [67 (link)].
9
Mouse Islet Hormone Modulation
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Mouse islets were isolated from 10 wild-type male mice and recovered overnight in complete medium: RPMI-1640 (Gibco) supplemented with 10% charcoal-stripped FBS and Pen/Strep (100 U/ml, 100 μg/mL). Approximately 250 islets were in each replicate and each condition was run in triplicate. Islets were treated with T (100nM; Sigma), the 5α-R inhibitors finasteride (100nM; Sigma) and dutasteride (100nM; Sigma), or vehicle (ethanol and DMSO). Other control conditions included culture medium without FBS and complete medium with finasteride and dutasteride. Culture medium and islets were harvested for further analysis after a 24-h incubation period.
10
Bacterial Culture Techniques and Reagents
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Bacterial strains used in this study are reported in Table 1 . Bacterial strains were routinely grown at 37°C in Luria-Bertani (LB) broth in shaking conditions, or in LB supplemented with 15 g/L agar.
When required, tetracycline (Tc; 200 μg/mL), isopropyl β-D-1-thiogalactopyranoside (IPTG), dimethyl sulfoxide (DMSO), or synthetic PQS were added to the medium. IPTG, DMSO and synthetic PQS were used at the concentrations indicated in the text. Synthetic PQS stock solution was prepared in MeOH at 20 mM concentration (synthetic PQS was kindly provided by Paul Williams and Miguel Càmara – University of Nottingham, United Kingdom). Ergotamine and Pimozide were available in our laboratory as drugs of the PHARMAKON library (10 mM stock solutions in DMSO). Dutasteride, eltrombopag and conivaptan were purchased from Sigma-Aldrich, Carbosynth Ltd., and MCE Medchem Express, respectively, and dissolved in DMSO at 10 mM concentration. Pimozide was also purchased from Sigma-Aldrich for further analyses, and dissolved in DMSO at 40 mM concentration.
When required, tetracycline (Tc; 200 μg/mL), isopropyl β-D-1-thiogalactopyranoside (IPTG), dimethyl sulfoxide (DMSO), or synthetic PQS were added to the medium. IPTG, DMSO and synthetic PQS were used at the concentrations indicated in the text. Synthetic PQS stock solution was prepared in MeOH at 20 mM concentration (synthetic PQS was kindly provided by Paul Williams and Miguel Càmara – University of Nottingham, United Kingdom). Ergotamine and Pimozide were available in our laboratory as drugs of the PHARMAKON library (10 mM stock solutions in DMSO). Dutasteride, eltrombopag and conivaptan were purchased from Sigma-Aldrich, Carbosynth Ltd., and MCE Medchem Express, respectively, and dissolved in DMSO at 10 mM concentration. Pimozide was also purchased from Sigma-Aldrich for further analyses, and dissolved in DMSO at 40 mM concentration.
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