K975x carbon coater

Manufactured by Quorum Technologies

The K975X Carbon Coater is a laboratory equipment used to apply thin carbon coatings to samples. It is designed to create uniform and controlled carbon films on the surface of specimens for various analytical techniques.

Automatically generated - may contain errors

Lab products found in correlation

Jem 2100 plus transmission electron microscope, by JEOL (1 mentions)

Close spelling variants of this product

Carbon coater

2 protocols using k975x carbon coater

Dehydration and SEM Imaging of Cultured Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cultures in well plates were dehydrated using increasing concentrations of ethanol (20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, 100% and 100% for 10 min each) and mounted on SEM stubs using carbon tape. Silver painting and carbon coating was carried out using a Quorum K975X Carbon coater. Samples were imaged on either a Hitatchi S—3499N or a Zeiss Sigma. For DDC-SEM images, secondary electron (SE) mode was used to obtain topographic information, whilst the backscattered electron detector (BSE) was applied to differentiate between organic and inorganic material. DDC-SEM images were produced by assigning different colours to the BSE and SE modes using Image J.

Loaiza S., Ferreira S.A., Chinn T.M., Kirby A., Tsolaki E., Dondi C., Parzych K., Strange A.P., Bozec L., Bertazzo S., Hedegaard M.A., Gentleman E, & Auner H.W. (2018). An engineered, quantifiable in vitro model for analysing the effect of proteostasis-targeting drugs on tissue physical properties. Biomaterials, 183, 102-113.

+ Open protocol

+ Expand

Nanoscale Sample Preparation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were silver painted and carbon coated using a Quorum K975X Carbon coater. For sample preparation, a FEI Helios NanoLab 600 DualBeam Focused Ion Beam System, at Imperial College London, was used. A micro region of each sample was coated with a Platinum layer at 30 kV and 93 pA. Following that, currents between 93 pA and 2.8 nA were used for section lift out and thinning of the sample to 100 nm. The sample was then placed on a carbon grid and transferred to a JEOL JEM 2100 Plus Transmission Electron Microscope at Imperial College London, to be imaged using 200 kV and 120 kV. Selected-area electron diffraction (SAED) was done at 200 kV. Image analysis were performed on ImageJ software.

Iacoviello F., Kirby A.C., Javanmardi Y., Moeendarbary E., Shabanli M., Tsolaki E., Sharp A.C., Hayes M.J., Keevend K., Li J.H., Brett D.J.L., Shearing P.R., Olivo A., Herrmann I.K., Evans S.E., Moazen M, & Bertazzo S. (2020). The multiscale hierarchical structure of Heloderma suspectum osteoderms and their mechanical properties. Acta biomaterialia, 107.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!