Micron 3 camera

Manufactured by Phoenix Pharmaceuticals

Sourced in United States

The Micron III camera is a high-performance imaging system designed for scientific and industrial applications. It features a high-resolution sensor, advanced image processing capabilities, and precise optical components. The camera's core function is to capture and digitize visual information with exceptional clarity and detail, enabling accurate analysis and documentation.

Automatically generated - may contain errors

Lab products found in correlation

Systane lubricant eye drops, by Alcon (2 mentions) Dmi4000b inverted fluorescence microscope, by Leica (1 mentions) Eb dye, by Merck Group (1 mentions) Fluorescein sodium, by Akorn (1 mentions) Ketamine, by Troy Laboratories (1 mentions) Xylazine, by Troy Laboratories (1 mentions) Mydriacil, by Alcon (1 mentions) Genteal gel, by Novartis (1 mentions) B6 sjl ptprca pepcb boyj, by Jackson ImmunoResearch (1 mentions) C cg foxp3tm2tch j foxp3egfp, by Jackson ImmunoResearch (1 mentions) B6 cg rag2tm1.1cgn j, by Jackson ImmunoResearch (1 mentions) Tropicamide, by Bausch & Lomb (1 mentions)

8 protocols using micron 3 camera

Fundus and Retinal Angiography in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fundus fluorescein angiography (FFA). Under anesthesia, mice were intravenously administered with sodium fluorescein (5 mg/kg of 10% w/v, Akorn, Lake Forest, IL). Fundus angiograms were obtained 2 min later at 490 nm with a Micron III camera (Phoenix, Pleasanton, CA, USA).
Retinal Evans Blue (EB) angiography. Briefly, mice were anesthetized; the descending aorta was clamped and then perfused via the left ventricle with 1 ml warm PBS containing EB dye (25 mg/kg of 1% solution, Sigma-Aldrich, CA, USA). The eyes were enucleated and placed in 4% paraformaldehyde for 2 h. Retinae were then dissected, flat-mounted in fluorescent mounting medium, and imaged with at 620 nm with a Leica DMI4000B inverted fluorescence microscope (Leica Microsystems, Wetzlar, Germany).

Zhu H., Zhang W., Zhao Y., Shu X., Wang W., Wang D., Yang Y., He Z., Wang X, & Ying Y. (2018). GSK3β-mediated tau hyperphosphorylation triggers diabetic retinal neurodegeneration by disrupting synaptic and mitochondrial functions. Molecular Neurodegeneration, 13, 62.

+ Open protocol

+ Expand

Focal ERG recordings in dark-adapted mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Focal ERG recordings were from dark-adapted mice (age P25), using a Micron-III camera-mounted focal ERG system (Phoenix Research Labs, Pleasanton CA). Aiming of the light stimulus was accomplished by viewing the retina with dim red-filtered LED illumination, high camera gain, and 15-frame video averaging. A circular LED white-light stimulus of 6 disc-diameters was projected onto the central retina (30-msec duration) with a bright intensity setting corresponding to an energy delivery of 121,597 cd-sec/m² of projected retinal area (measured by Greg Sprehn, Phoenix Research Labs, and Ken Mitton, ERI). Mice were maintained on a regulated temperature pad at 37 °C. The lens mount of the focal ERG provided the gold corneal electrode. Platinum cutaneous needle electrodes were used for the reference and ground and were inserted into the head cap and hind flank skin. Triggering of the light stimulus and acquisition of the corneal voltage trace were accomplished with LabScribe2 software equipped with the Phoenix Research Labs’ ERG module. Twenty stimulus traces were averaged to obtain the ERG trace. Amplitudes of the A-wave and B-wave were determined from averages of the left and right eyes for each mouse.

Mitton K.P., Guzman A.E., Deshpande M., Byrd D., DeLooff C., Mkoyan K., Zlojutro P., Wallace A., Metcalf B., Laux K., Sotzen J, & Tran T. (2014). Different effects of valproic acid on photoreceptor loss in Rd1 and Rd10 retinal degeneration mice. Molecular Vision, 20, 1527-1544.

+ Open protocol

+ Expand

Longitudinal Fundus Imaging in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were clinically examined on day (d) 0, 14, 21, 28 and 35 post-immunization (p.i.). Mice were anesthetized by an i.p. injection of a mixture of ketamine (80 mg/kg; Troy Laboratories, Glendenning, NSW, Australia) and xylazine (10 mg/kg; Troy Laboratories). The pupils were dilated with 0.5% tropicamide (Mydriacil, Alcon Laboratories, Vilvoorde, Belgium), and the cornea was kept moist with the application of sterile lubricant GenTeal gel (Novartis, North Ryde, NSW, Australia). The fundus was examined using the Micron III camera (Phoenix Research Laboratories, Pleasanton, CA, USA) with StreamPix 5 software. Examination consisted firstly of capturing short 5 seconds videos with 100 frames in brightfield, followed by a sequence of GFP⁺ or YFP⁺ cells using the green fluorescent barrier filters, and lastly by a short sequence following a 20 μl s.c. injection of 10% fluorescein isothiocyanate (Alcon Laboratories, Frenchs Forest, NSW, Australia) to visualize the retinal vasculature. The brightness and contrast of all fundus images were adjusted equally using ImageJ 1.48C software [27 ].

Chen X., Kezic J.M., Forrester J.V., Goldberg G.L., Wicks I.P., Bernard C.C, & McMenamin P.G. (2015). In vivo multi-modal imaging of experimental autoimmune uveoretinitis in transgenic reporter mice reveals the dynamic nature of inflammatory changes during disease progression. Journal of Neuroinflammation, 12, 17.

+ Open protocol

+ Expand

Generation and Characterization of Transgenic Mouse Models

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aire^GW/+ Lyn^−/− mice used in this study were previously described (20 (link)). B6.129P2-Rbp3 tm1Gil/J(IRBP^−/−)(stock no. 023080), B6(Cg)-Rag2^tm1.1Cgn/J(Rag2^−/−)(stock no 008449), C.Cg-Foxp3^tm2Tch/J(Foxp3^EGFP)(stock no 006769), and B6.SJL-Ptprc^a Pepc^b/BoyJ (stock no 002014) mice were obtained from the Jackson Laboratory. All animal experiments were approved by the UCSF Animal Care and Use Committee. P2.U2^+/− mice were generated by the transgenic core facility of the Gladstone Institutes, San Francisco, by microinjecting pCD2 TCR clonotype 2α chain and p428 TCR clonotype 2β chain DNA into C57BL/6 (Envigo, Strain, C57BL/6NHsd) embryos. Aire^GW/+ or WT CD45.1⁺/ CD45.2⁺ were from breeding of Aire^GW/+ with B6.SJL-Ptprc^a Pepc^b/BoyJ mice.
Intestinal microbes were depleted as previously described (33 (link)). The drinking water with MGVCK was given to pregnant dams and continued after weaning until mice were 9 wk of age.
Ocular funduscopy was performed as previous described (20 (link)) by using a Micron III camera (Phoenix Research Labs Inc.).

Yin M., Smith J.A., Chou M., Chan J., Jittayasothorn Y., Gould D.B., Caspi R.R., Anderson M.S, & DeFranco A.L. (2024). Tracking the role of Aire in immune tolerance to the eye with a TCR transgenic mouse model. Proceedings of the National Academy of Sciences of the United States of America, 121(5), e2311487121.

+ Open protocol

+ Expand

Measuring Intraocular Pressure in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

An Icare TonoLab rebound tonometer (Colonial Medical Supply, Windham, NH), a rodent-dedicated tonometer, was used for measuring intraocular pressure (IOP). IOP was determined in behaviorally trained conscious mice as described by Clark and coworkers (71 (link)). All IOP measurements were performed between 10 a.m. and 12 p.m. An average of 6 individual IOP measurements were taken to calculate the average final IOP value for each eye. All IOP measurements were recorded in a masked manner.
Fundus images were recorded using a Micron III camera per the manufacturer’s recommendations (Phoenix Research Labs, Pleasanton, CA).

Singh P.K., Kasetti R.B., Zode G.S., Goyal A., Juzych M.S, & Kumar A. (2019). Zika Virus Infects Trabecular Meshwork and Causes Trabeculitis and Glaucomatous Pathology in Mouse Eyes. mSphere, 4(3), e00173-19.

+ Open protocol

+ Expand

In vivo Retinal Morphology Examination

Check if the same lab product or an alternative is used in the 5 most similar protocols

Retinal morphology was examined in vivo by fundoscopy using a Micron III camera (Phoenix Research Laboratories, USA). Briefly, mice were anesthetized using chloral hydrate (400 mg/kg, i.p.). Pupils were dilated with 1% tropicamide and 2.5% phenylephrine. For maintenance of corneal moisture, Systane lubricant eye drops (Alcon, Inc., USA) were applied.

Wang B., Wang L., Gu S., Yu Y., Huang H., Mo K., Xu H., Zeng F., Xiao Y., Peng L., Liu C., Cao N., Liu Y., Yuan J, & Ouyang H. (2020). D609 protects retinal pigmented epithelium as a potential therapy for age-related macular degeneration. Signal Transduction and Targeted Therapy, 5, 20.

+ Open protocol

+ Expand

Alkali-Burn Corneal Neovascularization Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Alkali-burn treatments were conducted following previously published methods.²⁶ (link) Only the right eyes of mice were treated to conform to animal welfare standards required by IACUC and ARVO. Filter-paper discs (3-mm diameter) were pre-soaked in 1 M NaOH for 15 s and applied to eyes in experimental groups for 20 s. The ocular surface was then washed with 15 mL of normal saline for 1 min. A single investigator performed all of the described procedures to ensure reproducibility. Mouse corneas of anesthetized animals were imaged and acquired with a Micron III camera (Phoenix Research Labs, Pleasanton, CA, USA). The area of corneal NV was calculated by using the following formula modified from Liu et al.²⁷ (link) Area (mm²) = CN/12 × 3.1416 × [R² − (R − VL)²], where CN is the clock-hours of NV (1 clock-hour equals 30 degrees of arc); R is the radius of the cornea; and VL is the maximal vessel length, extending from the limbal vasculature. Measurements of corneas in live animals were performed five times each under a Micron III microscope, and the area of corneal NV was calculated accordingly.

Lu Y., Tai P.W., Ai J., Gessler D.J., Su Q., Yao X., Zheng Q., Zamore P.D., Xu X, & Gao G. (2018). Transcriptome Profiling of Neovascularized Corneas Reveals miR-204 as a Multi-target Biotherapy Deliverable by rAAVs. Molecular Therapy. Nucleic Acids, 10, 349-360.

+ Open protocol

+ Expand

Retinal Imaging in Mthfr Mutant Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Retinal morphology and vasculature were examined in vivo in Mthfr^+/+rd8/rd8 and Mthfr^+/−rd8/rd8 mice (8–52 weeks) by fundoscopy and fluorescein angiography (FA) using the Micron III camera (Phoenix Research Laboratories, Pleasanton, CA). Mice were anesthetized using ketamine/xylazine rodent anesthesia cocktail. Pupils were dilated with 1% tropicamide (Bausch & Lomb, Tampa, FL). For maintenance of corneal moisture, Systane lubricant eye drops (Alcon, Ft. Worth, TX) were applied. The mouse was positioned on the imaging stage for fundoscopy. For FA, the mouse was injected i/p with 10 to 20 μL fluorescein sodium (10% Lite; Apollo Ophthalmic, Newport Beach, CA). Fluorescent images were acquired rapidly at 30 sec intervals for ~ 5 minutes.

Markand S., Saul A., Tawfik A., Cui X., Rozen R, & Smith S.B. (2015). Mthfr as a modifier of the retinal phenotype of Crb1rd8/rd8 mice. Experimental eye research, 145, 164-172.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!