Total RNA (8–20 μg) from 21 hpf embryos and adults were isolated using RNAiso Reagent (Takara) and electrophoresed in a denaturing 12% polyacrylamide gel and transferred onto a Hybond-N+ membrane (GE Healthcare). miRNA probes (Additional file 5 : Table S3) were DIG-labeled LNA-modified probes synthesized by Exiqon (Denmark). Hybridization and washing were performed as described by Válóczi [36 (link)]. Signals were detected using CDP-Star chemiluminescent substrate (Roche), and the blot was exposed to the Lumi-Imager F1 Workstation. To control equal loading, blots were hybridized with a DIG-labeled probe against U6 snRNA.
Lumi imager f1 workstation
Manufactured by Roche
Sourced in Switzerland
The Lumi-Imager F1 Workstation is a compact, high-performance imaging system designed for sensitive luminescence detection. It features an integrated CCD camera, excitation light sources, and a temperature-controlled sample area. The Lumi-Imager F1 Workstation is capable of capturing images and quantifying luminescent signals with high sensitivity.
Automatically generated - may contain errors
Lab products found in correlation
Cdp star chemiluminescent substrate, by Roche (1 mentions)
Mirna probes, by Qiagen (1 mentions)
Hybond n membrane, by GE Healthcare (1 mentions)
Rnaiso reagent, by Takara Bio (1 mentions)
Amersham ecl prime kit, by GE Healthcare (1 mentions)
T 25 tissue culture flasks, by Thermo Fisher Scientific (1 mentions)
Anti v5, by Thermo Fisher Scientific (1 mentions)
Image gauge v4, by Fujifilm (1 mentions)
Anti β lactamase, by Abcam (1 mentions)
Ecl advance western blotting detection kit, by GE Healthcare (1 mentions)
Peroxidase conjugated anti mouse igg, by Merck Group (1 mentions)
3 protocols using lumi imager f1 workstation
1
Detecting miRNA Expression in Embryos
2
Flavonoid Pre-Treatment Effects on Protein Expression
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MDA-MB231 cells and LECs were grown in T-25 tissue culture flasks (Nunc, Roskilde, Denmark) to 80% confluence and then pre-treated with the flavonoids for 0.5, 1, 2, and 4 h. Then, cells were processed for SDS gel electrophoresis and Western blotting as described before (Nguyen et al., 2015 (link)). Chemo-luminescence was developed by Amersham ECL prime Kit (GE Healthcare, Freiburg, Germany) and detected using a Lumi-Imager F1 Workstation (Roche, Basel, Switzerland). Densitometry of the Western blots was analyzed with the Image-J software (National Institutes of Health, Bethesda, MD, United States).
3
Immunoblot Analysis of EnvZ-V5 Fusion Protein
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Pellets from cells
expressing EnvZ-V5 were analyzed on 12% SDS/acrylamide gels. Standard
buffers and conditions were used for electrophoresis and immunoblotting.39 Anti-V5 (Invitrogen) and anti-β-lactamase
(Abcam) primary antibodies were used. Peroxidase-conjugated anti-mouse
IgG (Sigma) was used as the secondary antibody. Bands were visualized
with the ECL advance western blotting detection kit (GE Healthcare).
Digitized images were acquired with a Lumi-Imager F1 workstation (Roche)
and analyzed with Image Gauge v4.22 software (Fujifilm). Intensities
of the β-lactamase bands served as an internal control for cell
density and sample loading.
expressing EnvZ-V5 were analyzed on 12% SDS/acrylamide gels. Standard
buffers and conditions were used for electrophoresis and immunoblotting.39 Anti-V5 (Invitrogen) and anti-β-lactamase
(Abcam) primary antibodies were used. Peroxidase-conjugated anti-mouse
IgG (Sigma) was used as the secondary antibody. Bands were visualized
with the ECL advance western blotting detection kit (GE Healthcare).
Digitized images were acquired with a Lumi-Imager F1 workstation (Roche)
and analyzed with Image Gauge v4.22 software (Fujifilm). Intensities
of the β-lactamase bands served as an internal control for cell
density and sample loading.
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