Plasma concentrations of alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were measured using optimized UV at 340 nm, and insulin concentrations were measured using a rat insulin radioimmunoassay kit at 4 °C (Linco Research Inc, St Charles, MO). Plasma glucose and triglyceride were measured colorimetrically, Gamma glutamyltransferase (GGT), and Alkaline phosphatase (ALP) assessed photometrically, total Cholestrol, HDL, and LDL cholesterol were examined enzymatically all by using a commercial kit (Parsazmoon, Tehran, Iran).
Rat insulin radioimmunoassay kit
Manufactured by Linco Research
Sourced in United States
The Rat Insulin Radioimmunoassay (RIA) Kit is a laboratory tool used to quantify the concentration of insulin in rat biological samples. It employs the radioimmunoassay technique to measure insulin levels accurately.
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Lab products found in correlation
4 protocols using rat insulin radioimmunoassay kit
1
Plasma Biomarker Measurement Protocols
2
Plasma Glucose and Insulin Measurement
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The plasma glucose level (mmol/L) was measured by a glucose kit (Sigma Chemical Co., St. Louis, MO, USA). Plasma insulin level (nmol/L) was measured by using a rat insulin radioimmunoassay kit (Linco Research Inc., St. Charles, MO, USA).
3
Metabolic Biomarker Measurement Protocol
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The urine and plasma samples were analyzed for sodium and potassium concentrations using a flame photometer 480 (Ciba Corning Diagnostics, Norwood, MA). Creatinine levels in the plasma and urine were measured using a creatinine analyzer (Beckman, Fullerton, CA). The glomerular filtration rate (GFR) (mL/min) was estimated by creatinine clearance.28 (link) Blood glucose was measured with a glucose analyzer (Roche, Indianapolis, IN), and plasma insulin was measured by rat insulin radioimmunoassay kit (Linco Research, St. Charles, MO). Triglycerides were measured by a triglyceride analyzer (Polymer Technology Systems, Cardiochek, IN). Hematocrit (Hct) was measured by a micro‐Hct centrifuge (Haemofuge Heraeus Instr, Germany).
4
Lipid and Inflammatory Biomarkers Measurement
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Plasma concentrations of ALT, AST, GGT, ALP, and serum lipids, including TC, HDL,
LDL, and TG were measured by applying Parsazmoon kits (Tehran, Iran) in Noor
pathobiology laboratory (Tehran, Iran). Also, insulin concentrations were
measured using a rat insulin radioimmunoassay kit at 4°C (Linco Research Inc).
Plasma glucose was measured through colorimetric methods by using a commercial
kit (Parsazmoon). Eliza kit specifically for the rat was used to determine
high-sensitivity C-reactive protein (hs-CRP) (hs-CRP; Cusabio Biothech), total
antioxidant capacity (TAC) was measured according to Koracevic et al, and
HOMA-IR scores were calculated using fasting serum insulin and glucose
concentrations at the end of the experimental period according to the following
formula: (20. Insulin/glucose-3.5) %.
LDL, and TG were measured by applying Parsazmoon kits (Tehran, Iran) in Noor
pathobiology laboratory (Tehran, Iran). Also, insulin concentrations were
measured using a rat insulin radioimmunoassay kit at 4°C (Linco Research Inc).
Plasma glucose was measured through colorimetric methods by using a commercial
kit (Parsazmoon). Eliza kit specifically for the rat was used to determine
high-sensitivity C-reactive protein (hs-CRP) (hs-CRP; Cusabio Biothech), total
antioxidant capacity (TAC) was measured according to Koracevic et al, and
HOMA-IR scores were calculated using fasting serum insulin and glucose
concentrations at the end of the experimental period according to the following
formula: (20. Insulin/glucose-3.5) %.
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