Batch experiments and column leachate experiments were performed on the soils that were most polluted with Sb (S9). Experiments were performed in a 50 mL centrifuge tube with four grams of untreated soil or soil amendment mix (0%, 2%, 5%, and 10% iron-based adsorbent; 0%, 2%, and 4% aluminum-based adsorbent) and the corresponding volume of deionized water was treated with a Milli-Q water purification device (Millipore Corp, Billerica, MA, USA) with a liquid-to-solid ratio of 10 (L/S). Centrifuge tubes were shaken at 100 rpm/min for 10 days at room temperature (25 ± 1 °C) in an incubator shaker. Based on the earlier sorption studies of Sb to Fe-based materials, 10 days reaction time was sufficient to reach equilibrium [15 (link),41 ]. All experiments were performed in triplicate. After reaction, the samples were centrifuged, and the supernatant was filtered with a 0.45 μm filter membrane (PES, ReLAB). The filtrate was used for Sb, Cu, and Zn analysis.
Milli q water purification device
Manufactured by Merck Group
Sourced in United States
The Milli-Q water purification device is a laboratory equipment designed to produce high-quality, ultra-pure water. The core function of the device is to remove impurities, such as organic compounds, heavy metals, and ions, from the input water source, resulting in a consistent supply of highly purified water for various laboratory applications.
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Lab products found in correlation
Ammonium formate, by Merck Group (2 mentions)
Malic acid, by Merck Group (2 mentions)
D3 m6a, by LGC (2 mentions)
D3 um, by LGC (2 mentions)
Masshunter workstation b 06, by Agilent Technologies (1 mentions)
Hplc grade formic acid, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Mueller hinton broth (mhb), by BD (1 mentions)
Mueller hinton agar (mha), by BD (1 mentions)
Agilent 1260 high performance liquid chromatography system, by Agilent Technologies (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Uridine, by LGC (1 mentions)
Adenosine, by LGC (1 mentions)
D3 m1a, by LGC (1 mentions)
Chromatographic grade acetonitrile, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Guangzhou Chemical (1 mentions)
Hplc grade methanol meoh, by Merck Group (1 mentions)
7 protocols using milli q water purification device
1
Soil Remediation of Antimony Pollution
2
Quantification of Tigecycline in Plasma
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Tigecycline reference standard (99% purity, Lot L910S56 and LN70S134) was purchased from Beijing J&K Scientific Ltd., and internal standard (IS) tigecycline-d9 (95% purity, Lot 2-NYL-17-1-PFZ) was purchased from Toronto Research Chemical, Inc., HPLC-grade formic acid and methanol were purchased from Thermo Fisher Scientific. Ultrapure water was prepared using a Milli-Q water purification device (Millipore, Bedford, MA, United States). Drug-free plasmas were obtained from the Department of Blood Transfusion, PLA General Hospital. A total of 134 AB isolates were used for susceptibility tests. Quality control strain ATCC25922 was purchased from National Institutes for Food and Drug Control, China. Mueller–Hinton Agar and Mueller–Hinton Broth were purchased from Becton, Dickinson and Company. Chromatographic analysis was performed using the Agilent 1260 high-performance liquid chromatography system (Agilent Technologies Inc.), and mass spectral analysis was performed using the Agilent 6460A mass spectrometer (Agilent Technologies Inc.) with an Agilent MassHunter Workstation B.06.00 software for data processing.
3
Quantitative Analysis of Nucleosides
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Acetonitrile of chromatographic grade was purchased from Merck KGaA (Darmstadt, Germany). A solution of formic acid (HCOOH) was purchased from Fluka (Muskegon, USA). Ammonium formate and malic acid were obtained from Sigma-Aldrich (St Louis, MO, United States). Adenosine, N6-methylAdenosine, N1-methylAdenosine, 2′-O-methylguanosine (Gm), N1-methylguanosine, N2-methylguanosine (m2G), 2′-O-methylcytidine, uridine, 3-methyluridine (m3U), 5-methyluridine (m5U), and 2′-O-methyluridine and isotopically labeled standards, including [13C5] A, [D3] m6A, [D3] m1A, [D3] Cm, [13CD3] m5C, [13C5N2] U, [D3] Um, [13C5] m5U, and [D6] m2,2G, were purchased from Toronto Research Chemicals (Toronto, Canada). In order to purify water, a Milli-Q water purification device was used (Millipore, Milford, MA, United States).
4
Quantification of Bioactive Compounds in G. rhizoma
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Totally, 13 batches of raw G. rhizoma samples were provided by Guizhou Jiulong Group (Guizhou, China). Reference standards of gastrodin (batch No. 110807–201608), p-hydroxybenzyl alcohol (batch No. 111970–201702), parishin A (batch No. 62499-28-9), and parishin B (batch No. 174972-79-3) were purchased from China Food and Drug Testing Institute (Beijing, China). Methanol and acetonitrile were purchased from Kemmo Chemical Reagent (Tianjin, China). Water was purified by a Milli-Q water purification device (Millipore, USA). Other chemical reagents were all analytical grade.
5
Analysis of Green Tea Compounds
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A total of 39 batches of green tea samples were supplied by Qiannan Meiyuan Trade Co., Ltd. (Guizhou, China). Reference substance of caffeine (batch No. wkq20041403) and EGCG (batch No. wkq17122008) was purchased from Sichuan Weiqi Biological Technology Co., Ltd. (Sichuan, China). Methanol and acetonitrile were purchased from Kemmo Chemical Reagent (Tianjin, China). Water was purified by using a Milli-Q water purification device (Millipore, USA). The other chemical reagents were all of analytical grade.
6
Quantification of RNA Modifications
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Chromatographic grade acetonitrile was bought from Merck KGaA (Darmstadt, Germany). Methanol of HPLC grade was purchased from J.T.Baker (Radnor, PA, United States). Formic acid (HCOOH) was bought from Fluka (Muskegon, United States). Ammonium formate, malic acid and 5-methylcytidine (m5C) were bought from Sigma-Aldrich (St Louis, MO, United States). 2′-O-methyladenosine (Am), N6-methyladenosine (m6A), 2′-O-methylguanosine (Gm), 1-methylguanosine (m1G), 2′-O-methylcytidine (Cm), 2′-O-methyluridine (Um), 5-methyluridine (m5U) and isotopically labeled standards [D3]m6A, [D3]Um and [13C5]m5U were obtained from Toronto Research Chemicals (Toronto, Canada). [13C15N2]Gm, [13C15N2]m1G and [13C5]Cm were synthesized according to literature (Fu et al., 2015 (link)). Water was purified by a Milli-Q water purification device (Millipore, Milford, MA, United States).
7
Quantitative Analysis of Magnolia Bioactive Compounds
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HPLC-grade methanol (MeOH) was purchased from Merck (Darmstadt, Germany). Diethylamine and hydrochloric acid (HCl) were obtained from Guangzhou Chemical Reagent Factory (Guangzhou, China). Three reference standards (tetrandrine, columbamine, berbamine) and reference extract of M. bealei were supplied by National Institutes for Food and Drug Control (Beijing, China). Purified water was from a Milli-Q water purification device (Millipore, Billerica, MA, USA). The detailed sample information is listed in Table 3 .
All cultivated M. bealei samples were authenticated by TCM testing department (Shenzhen Institute for Drug Control, Guangdong, China) and wild M. bealei were authenticated by Prof. Weixian Deng (Shaoguan University, Guangdong, China). All the samples were randomly divided into training set, validation set and predication set. As a result, the training set consisted of 37 samples including S1–S27, S31, S34, S36, S39–S42, S46–S47 and S49 to establish a classification model. 26 samples including S33, S35, S37–S38, S48, S51–S71 were used as testing set for model validation while another 26 samples including S28–S30, S32, S43–S45, S50, S72–S89 were applied as prediction set to verify the prediction ability.
All cultivated M. bealei samples were authenticated by TCM testing department (Shenzhen Institute for Drug Control, Guangdong, China) and wild M. bealei were authenticated by Prof. Weixian Deng (Shaoguan University, Guangdong, China). All the samples were randomly divided into training set, validation set and predication set. As a result, the training set consisted of 37 samples including S1–S27, S31, S34, S36, S39–S42, S46–S47 and S49 to establish a classification model. 26 samples including S33, S35, S37–S38, S48, S51–S71 were used as testing set for model validation while another 26 samples including S28–S30, S32, S43–S45, S50, S72–S89 were applied as prediction set to verify the prediction ability.
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