and acetonitrile (MeCN) of analytical grade were purchased from Panreac
(Castellar del Vallès, Spain). Formic acid (HCOOH), sodium
dihydrogenphosphate (NaH2PO4), and sodium hydroxide
(NaOH), all of analytical grade, were supplied by Merck (Darmstadt,
Germany). Water (18.2 MΩ·cm) was generated using a Milli-Q
purification system from Millipore (Billerica, MA, USA).
The
buffer solution of sodium dihydrogenphosphate (NaH2PO4; 0.1 mol·L–1) used in the treatment
of samples was adjusted to pH 10 with 5 mol·L–1 NaOH. Oasis HLB polymeric cartridges (3 cm3, 60 mg) from
Waters (Milford, MA, USA) were used for solid-phase extraction. Membrane
filters (Ultra free Durapore PVDF 0.22 μm) from Millipore were
used to filter the extract before analysis.