S monovette serum gel tubes

Manufactured by Sarstedt

Sourced in Germany

The S-Monovette Serum-Gel tubes are blood collection tubes manufactured by Sarstedt. The tubes contain a gel separator that allows for the separation of serum from the cellular components of the blood sample during centrifugation.

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Lab products found in correlation

S monovette plasma edta tubes, by Sarstedt (2 mentions) Forane, by Abbott (2 mentions) Tempus blood rna tube, by Thermo Fisher Scientific (1 mentions) Coulter ac t diff hematology analyzer, by Beckman Coulter (1 mentions) S monovette k3 edta tubes, by Sarstedt (1 mentions)

10 protocols using s monovette serum gel tubes

Collecting Blood and Placental Samples

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Blood samples were collected at the time of delivery. A total of 5 ml venous blood was drawn in S-Monovette serum-gel tubes (Sarstedt, Germany). The samples were centrifuged at 1000 g for 10 min at 4 °C. The supernatant serum was transferred in aliquots to 1.5 ml Eppendorf tubes and frozen at − 80 °C until analysed.
Placental tissues were obtained within 15 min after delivery. Samples (approximately 1 cm
³each) were dissected from three different sites of the maternal side under aseptic conditions. Biopsy cores were snap frozen in liquid nitrogen and further stored at − 80 °C until analysis.

Kasoha M., Takacs Z., Fackiner L., Gerlinger C., Sklavounos P., Radosa J., Solomayer E.F, & Hamza A. (2021). Comparison of Maternal Serum Levels and Placental mRNA Levels of Dickkopf-1 in Preeclamptic and Normal Pregnant Women at Delivery. Geburtshilfe und Frauenheilkunde, 81(11), 1247-1255.

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Blood Collection Protocol for Isoflurane Anesthesia

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At the end of the 10-month study period, blood was drawn by heart puncture under deep isoflurane anaesthesia (Forane, Abbott, Austria). Also for the baseline animals, the blood was collected only once at the time of sacrification. Blood and plasma were collected using S-Monovette Serum-Gel tubes and S-Monovette Plasma-EDTA tubes (Sarstedt, Nümbrecht, Germany), respectively. Samples were centrifuged at 2000 g for 12 min at room temperature, aliquoted and stored at − 80 °C until the analysis. Blood collections and consequently serum analyses were performed in a non-fasting state.

Semeraro M.D., Almer G., Kaiser M., Zelzer S., Meinitzer A., Scharnagl H., Sedej S., Gruber H.J, & Herrmann M. (2021). The effects of long-term moderate exercise and Western-type diet on oxidative/nitrosative stress, serum lipids and cytokines in female Sprague Dawley rats. European Journal of Nutrition, 61(1), 255-268.

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Antibody Response in BNT162b2 Vaccinated

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Blood samples of fully BNT162b2 vaccinated individuals (n=5, three females, two males, age range 27-61 years, average age 42.2 years) were obtained after the participants information and written consent. Samples were collected 13−30 days after the second vaccination using S-Monovette Serum Gel tubes (Sarstedt). Before use, the serum was heat-treated at 56 °C for 30 min. Ulm University Medical Center Employees who were vaccinated twice, had no indication of previous SARS-CoV-2 infection and expressed interest in participating were included the present study. There were no further inclusion/exclusion parameters. Ethics approval was provided by the Ethic Committee of Ulm University (vote 99/21– FSt/Sta).

Pastorio C., Zech F., Noettger S., Jung C., Jacob T., Sanderson T., Sparrer K.M, & Kirchhoff F. (2022). Determinants of Spike infectivity, processing, and neutralization in SARS-CoV-2 Omicron subvariants BA.1 and BA.2. Cell Host & Microbe, 30(9), 1255-1268.e5.

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Blood and CSF Collection Protocol

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From each patient, venous blood was drawn in the morning between 8 a.m. and 9 a.m. into S-Monovette^® Serum-Gel Tubes (Sarstedt, Nümbrecht, Germany) and Tempus™ Blood RNA Tubes (Thermo Fisher Scientific, Waltham, MA, USA). For the collection of sera, whole blood was left to clot for 30 min at room temperature and later centrifuged for 10 min at 1200 g. Supernatant was aliquoted and stored at -20°C until further analysis. Tempus™ Blood RNA Tubes were immediately shaken for 30 s to lyse the cells and stabilize the RNA and stored at -80°C until extraction.
CSF samples were obtained after a lumbar puncture at the L4/5 level. The amount of CSF taken varied between 10-15 ml. Samples of 2 ml were aliquoted and subsequently stored at -20 °C.

García-Fernández P., Höfflin K., Rausch A., Strommer K., Neumann A., Cebulla N., Reinhold A.K., Rittner H., Üçeyler N, & Sommer C. (2023). Systemic inflammatory markers in patients with polyneuropathies. Frontiers in Immunology, 14, 1067714.

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Standardized Blood Collection and Metabolic Profiling

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In KORA, blood was drawn into S-Monovette® serum gel tubes (SARSTEDT AG & Co., Nümbrecht, Germany) in the morning between 8:00 a.m. and 10:30 a.m. after a fasting period of at least eight hours [17 (link)]. Blood samples were processed according to standard procedures. The samples were stored at −195.79 °C in liquid nitrogen until the execution of metabolic analyses. Details were reported in previous publications [11 (link),17 (link)].
In CARLA, metabolic profiles in the blood serum of the study participants were measured. Blood samples were taken after a supine rest of 30 min. Followed by a 10 min centrifugation (20 °C, 1500 revolutions per min), the samples was collected and deep frozen to −80 °C on the same day until analysis of the metabolites [45 (link)].

Chak C.M., Lacruz M.E., Adam J., Brandmaier S., Covic M., Huang J., Meisinger C., Tiller D., Prehn C., Adamski J., Berger U., Gieger C., Peters A., Kluttig A, & Wang-Sattler R. (2019). Ageing Investigation Using Two-Time-Point Metabolomics Data from KORA and CARLA Studies. Metabolites, 9(3), 44.

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SARS-CoV-2 Antibody Response in Vaccinated

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Sera from individuals vaccinated with BioNTech/Pfizer vaccine BNT162b2 were obtained 13-15 days after the second dose. The study was approved by the Ethic committee of Ulm university (vote 31/21 – FSt/Sta). Collection of plasma samples from COVID-19 patients treated at the intensive care unit was approved by the Ethic committee of the University Medicine Göttingen (SeptImmun Study 25/4/19 Ü). For collection of plasma, Cell Preparation Tube (CPT) vacutainers with sodium citrate were used and plasma was collected as supernatant over the PBMC layer. For vaccinated patients, blood was collected in S-Monovette® Serum Gel tubes (Sarstedt). Subsequently, the plasma and serum samples were incubated at 56°C for 30 min to inactivate putative infectious virus. For convalescent plasma, pre-screening for detection of neutralizing activity was performed on Vero76 cells using SARS-2-S- and VSV-G bearing pseudotypes as control, normalized for equal infectivity.

Hoffmann M., Arora P., Groß R., Seidel A., Hörnich B.F., Hahn A.S., Krüger N., Graichen L., Hofmann-Winkler H., Kempf A., Winkler M.S., Schulz S., Jäck H.M., Jahrsdörfer B., Schrezenmeier H., Müller M., Kleger A., Münch J, & Pöhlmann S. (2021). SARS-CoV-2 variants B.1.351 and P.1 escape from neutralizing antibodies. Cell, 184(9), 2384-2393.e12.

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Blood Sample Collection and PBMC Isolation

Cited 1 time

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We obtained approximately 30 ml of blood in S-Monovette K3 EDTA tubes and 7 ml in S-Monovette Serum-Gel tubes (Sarstedt). All samples were collected in the morning (8:00 a.m.). PBMCs were then isolated using a Ficoll–Hypaque gradient as described (17 (link)), aliquoted in RPMI containing 10% DMSO and 25% FCS at 1 × 10⁷ cells/ml, gradually cooled down to −80°C in a Mr. Frosty for 18 h and stored in liquid nitrogen until assayed. A small amount of whole blood (50 µl) was used for total and differential leukocyte counts using a Coulter Ac·T Diff hematology analyzer (Beckman Coulter). All other assays were performed using cryopreserved PBMCs or serum with one subject from each group run in parallel to control systematic variation in reagents used.

Patas K., Willing A., Demiralay C., Engler J.B., Lupu A., Ramien C., Schäfer T., Gach C., Stumm L., Chan K., Vignali M., Arck P.C., Friese M.A., Pless O., Wiedemann K., Agorastos A, & Gold S.M. (2018). T Cell Phenotype and T Cell Receptor Repertoire in Patients with Major Depressive Disorder. Frontiers in Immunology, 9, 291.

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Comparison of Vaccine-Induced Immunity

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Blood samples of ChAdOx1-nCoV-19/ BNT162b2/ BNT162b2 and BNT162b2/ BNT162b2/ BNT162b2 vaccinated non-convalescent individuals were obtained after the participants information and written consent. Samples were collected 11–15 days after the third dose using S-Monovette Serum Gel tubes (Sarstedt). Before use, the serum was heat-treated at (56 °C, 30 min). Ethics approval was given by the Ethics Committee of Ulm University (vote 99/21– FSt/Sta). All ethical regulations relevant to human research participants were followed.

Wang Q., Noettger S., Xie Q., Pastorio C., Seidel A., Müller J.A., Jung C., Jacob T., Sparrer K.M., Zech F, & Kirchhoff F. (2023). Determinants of species-specific utilization of ACE2 by human and animal coronaviruses. Communications Biology, 6, 1051.

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Myocardial Tissue Sampling Protocol

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At the end of the 10-months study protocol, blood was drawn by heart puncture under deep isoflurane anesthesia (Forane, Abbott, Austria). Blood and plasma were collected using S-Monovette Serum-Gel tubes and S-Monovette Plasma-EDTA tubes (Sarstedt, Nümbrecht, Germany), respectively. Blood collection was performed in a non-fasting state. From 10 animals per group, the base and mid-ventricular portion of the myocardium were formalin-fixed for 24 h and stored in EtOH at 4°C until analysis. The samples have been rehydrated overnight in PBS at 4°C before sectioning. The apical portion of the ventricles was snap frozen from all animals and stored at −80°C for subsequent molecular analyses.

Semeraro M.D., Beltrami A.P., Kharrat F., Almer G., Sedej S., Renner W., Gruber H.J., Curcio F, & Herrmann M. (2023). The impact of moderate endurance exercise on cardiac telomeres and cardiovascular remodeling in obese rats. Frontiers in Cardiovascular Medicine, 9, 1080077.

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Serum Antibody Response to COVID-19 Vaccines

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Blood samples of ChAdOx1-nCoV-19/BNT162b2 and BNT162b2 vaccinated individuals were obtained after the participants information and written consent. Samples were collected 13−30 days after the second dose using S-Monovette Serum Gel tubes (Sarstedt). Before use, the serum was heat-treated at (56 °C, 30 min). Ethics approval was given by the Ethic Committee of Ulm University (vote 99/21– FSt/Sta).

Zech F., Schniertshauer D., Jung C., Herrmann A., Cordsmeier A., Xie Q., Nchioua R., Prelli Bozzo C., Volcic M., Koepke L., Müller J.A., Krüger J., Heller S., Stenger S., Hoffmann M., Pöhlmann S., Kleger A., Jacob T., Conzelmann K.K., Ensser A., Sparrer K.M, & Kirchhoff F. (2021). Spike residue 403 affects binding of coronavirus spikes to human ACE2. Nature Communications, 12, 6855.

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